核桃楸皮提取物对人肝癌细胞SMMC-7721的增殖抑制作用及对细胞周期的影响

doi:10.3969/j.issn.0253-2417.2017.03.019

Abstract

Abstract: To study effect of extracts from bark of Juglans mandshurica (EMBJM) on proliferation and cell cycle of SMMC-7721 cells, the SMMC-7721 cells in culture medium in vitro were given different concentrations of EMBJM. The inhibition rate of EMBJM on the SMMC-7721 cell was detected by MTT assay. The cell cycle analyzed was detected by flow cytometry (FCM). The EMBJM exhibited a significant inhibition on SMMC-7721 cells time-and dose-dependently. The cell cycle detection results suggested that EMBJM could arrest SMMC-7721 cells specifically in growth 2/mitosis(G2/M) and synthesis(S) phase cell cycle in a dose-dependent manner. Compared with control group (8.34%±1.42%; 26.80%±1.76%), the G2/M and S phase of SMMC-7721 cells exposed to EMBJM (13.89%±2.46%, 39.87%±2.83% (8 mg/L);(14.35%±2.68%, 44.81%±2.93% (16 mg/L)) were significantly increased (P<0.05, n=3). EMBJM can effectively inhibited proliferation in SMMC-7721 cells and effected cell cycle distribution.

Key words: bark of Juglans mandshurica, cytotoxicity, cell cycle, SMMC-7721 cells

CLC Number:

TQ35

LIANG Qichao, LIU Shuang, ZHANG Zhaoli. Effect of Extracts from Bark of Juglans mandshurica on Proliferation and Cell Cycle of SMMC-7721 Cells[J]. Chemistry and Industry of Forest Products, 2017, 37(3): 136-140.

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Effect of Extracts from Bark of Juglans mandshurica on Proliferation and Cell Cycle of SMMC-7721 Cells

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