响应面法优化透析制备低分子质量人参糖肽的工艺

doi:10.3969/j.issn.0253-2417.2013.02.006

›› 2013, Vol. 33 ›› Issue (2): 37-40.doi: 10.3969/j.issn.0253-2417.2013.02.006

• 研究报告 • Previous Articles Next Articles

Optimized Dialysis Preparation of Low Molecular Mass Glycopeptide from Panax ginseng Using the Response Surface Methodology

WANG Ying¹, LUO Hao-ming², CHEN Ying-hong¹, JIANG Rui-zhi¹

1. Jilin Academy of Chinese Medicine and Material Medica Science, Changchun 130012, China;2. College of Pharmacy, Jilin University, Changchun 130021, China

Received:2012-03-19 Revised:1900-01-01 Online:2013-04-30 Published:2013-04-30

Abstract

Abstract: Response surface methodology (RSM) was used to study the technology of preparation of low molecular mass glycopeptide from panax ginseng by dialysis. The effects of three factors, i.e., the ultrafiltrate concentration before dialysis (A), dialysis time (B) and water consumption (C) on the content of glycopeptide were investigated. The optimal conditions for dialysis were ultrafiltrate concentration 0.6 g/mL, dialysis time 5 h 1 min 48 s and water consumption 3.92 L. The experimental values (79.26 %) agree with the predicted values(78.99 %). The panax ginseng peptide with relatively high purity can be prepared by dialysis. A model equation obtained can be used to predict the experiment through the RSM.

Key words: Panax ginseng, glycopeptide, dialysis, response surface metnod (RSM)

CLC Number:

TQ35
O657.3

WANG Ying;LUO Hao-ming;CHEN Ying-hong;JIANG Rui-zhi. Optimized Dialysis Preparation of Low Molecular Mass Glycopeptide from Panax ginseng Using the Response Surface Methodology[J]. , 2013, 33(2): 37-40.

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Optimized Dialysis Preparation of Low Molecular Mass Glycopeptide from Panax ginseng Using the Response Surface Methodology

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