关键词: 黑曲霉, 里氏木霉, 混合培养, β-葡萄糖苷酶

Abstract: Aspergillus NL02 and Trichoderma reesei RUT-C30 were co-cultured to produce β-glycosidase by fermentation. The effects of factors, including carbon source, nitrogen source, carbon- nitrogen ratio, pH value, loading volume, temperature, inoculum size, inoculum time and vaccination proportion of these two enzymes on the activity of β-glycosidase were investigated and optimized. The results showed that the optimum culture medium was described as follows: bran 15 g/L, corncob 35 g/L, ammonium sulfate 6.35 g/L, initial pH 5.5, the active spores access quantity 10⁷ spores/mL, loading volume 50 mL in a 250 mL triangle bottle, temperature 40℃, rotate speed 170 r/min in shaker and Aspergillus and T.reesei ratio at 5:1. Under this culture condition, the β-glycosidase with 28.15 IU/mL enzyme activity was obtained. In contrast to the sample cultured by Aspergillus alone (24.80 IU/mL), a 13.5% increase of enzyme activity was observed.

Key words: Aspergillus, Trichoderma reesei, co-cultured, β-glycosidase