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林产化学与工业

林产化学与工业 ›› 2011, Vol. 31 ›› Issue (6): 73-77.

• 研究报告 • 上一篇    下一篇

松树皮多酚的亚临界水提取及抗氧化活性初探

薄采颖1, 郑光耀1, 陈琰2, 周维纯1, 李若达3, 谢学容3   

  1. 1. 中国林业科学研究院 林产化学工业研究所;生物质化学利用国家工程实验室; 国家林业局 林产化学工程重点开放性实验室, 江苏 南京 210042;2. 中国药科大学 药理学教研室, 江苏 南京 210009;3. 中健行集团有限公司, 广东 乐昌 512229
  • 收稿日期:2011-07-09 修回日期:1900-01-01 出版日期:2011-12-30 发布日期:2011-12-30
  • 通讯作者: 郑光耀,副研究员,从事植物资源提取分离与开发利用研究;E-mail:zhguya@sina.com。

Preliminary Study on Subcritical Water Extraction and Antioxidant Activity of Polyphenols from Pine Bark

BO Cai-ying1, ZHENG Guang-yao1, CHEN Yan2, ZHOU Wei-chun1, LI Ruo-da3, XIE Xue-rong3   

  1. 1. Institute of Chemical Industry of Forest Products,CAF;National Engineering Lab.for Biomass Chemical Utilization; Key and Open Lab.on Forest Chemical Engineering,SFA, Nanjing 210042, China;2. Dep of Pharmacology,China Pharmaceutical University, Nanjing 210009, China;3. Zhongjianxing Group Corporation Limited, Lechang 512229, China
  • Received:2011-07-09 Revised:1900-01-01 Online:2011-12-30 Published:2011-12-30

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摘要： 采用亚临界水从松树皮中提取多酚,考察了反应条件对多酚得率的影响,并与超声波辅助提取法和热回流提取法进行了比较,同时测定了亚临界水提取所得松树皮提取物的DPPH自由基清除能力。结果表明:亚临界水提取松树皮多酚的最佳工艺条件为:提取温度 150 ℃、提取时间 5 min、液料比20∶1(mL∶g)、提取压力 4 MPa,多酚的提取得率为 4.86 %,1次提取且相同液料比条件下,比超声波辅助提取法和热回流提取法分别提高了 33.52 % 和 37.68 %。亚临界水提取所得松树皮提取物对DPPH自由基具有良好的清除效果,其半数抑制质量浓度(IC50)为 7.10 mg/L,抗氧化性高于常用抗氧化剂BHA(7.82 mg/L)。

关键词: 松树皮, 亚临界水, 多酚, DPPH

Abstract: Subcritical water was used to extract polyphenol from pine bark. Its effects on the polyphenol yield were investigated.The DPPH radical scavenging activity of polyphenol by subcritical water extraction was determined. The results showed that the optimum process conditions were extraction temperature 150 ℃, extraction time 5 min, liquid-feed ratio 20∶1, and extraction pressure 4 MPa. Under this condition the yield of polyphenol was 4.86 %. Compared with the ultrasound-assisted extraction and heat reflux extraction, the subcritical water extraction improved the yields of polyphenol by 33.52 % and 37.68 %, respectively. The polyphenol extracted by subcritical water had a better DPPH radicals scavenging activity than that of the antioxidant BHA(7.82 mg/L). The half inhibitory concentration (IC50) of the polyphenol was 7.10 mg/L.

Key words: pine bark, subcritical water, polyphenol, DPPH

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