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林产化学与工业 ›› 2004, Vol. 24 ›› Issue (3): 87-89.

• 研究报告 • 上一篇    下一篇

二次纤维酶水解生成葡萄糖的研究

杭志喜   

  1. 安徽工程科技学院 生物化学工程系, 安徽 芜湖 241000
  • 收稿日期:2003-11-24 修回日期:1900-01-01 出版日期:2004-09-30 发布日期:2004-09-30

STUDY ON ENZYMATIC HYDROLYSIS OF CELLULOSE FROM SECONDARY FIBERS TO GLUCOSE

HANG Zhi-xi   

  1. Dept.of Bioch. Engn., Anhui University of Technology and Science, Wuhu 241000, China
  • Received:2003-11-24 Revised:1900-01-01 Online:2004-09-30 Published:2004-09-30

摘要: 将回收的废纸脱墨、洗净即为二次纤维。脱去其中的木质素和半纤维素,得纤维素,用纤维素酶水解生成葡萄糖。对温度、pH值、酶用量、时间分别进行单因素试验,通过测定水解液葡萄糖含量,找出水解的适宜条件:温度45℃,pH值4.8,酶用量8IU/g(对绝干原料),产率可达60%以上。

关键词: 纤维素酶, 纤维素, 葡萄糖, 二次纤维, 糖化

Abstract: After de-inking and washing-off,the waste paper is called secondary fiber.Lignin and hemicellulose are removed from secondary fiber to obtain cellulose,which is hydrolyzed by cellulase to obtain glucose.The temperature,pH value,dosage of enzyme and time are experimented by measuring the content of glucose to find out the optimal condition of enzymatic hydrolysis:temperature 45℃,pH value 4.8,dosage 8 IU/g(based on dry material).The product yield may exceed 60%.

Key words: cellulase, cellulose, glucose, secondary fibers, saccharification

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