没食子单宁降解菌株的筛选及发酵条件优化

doi:10.3969/j.issn.0253-2417.2014.05.014

林产化学与工业 ›› 2014, Vol. 34 ›› Issue (5): 84-90.doi: 10.3969/j.issn.0253-2417.2014.05.014

没食子单宁降解菌株的筛选及发酵条件优化

闵凡芹¹, 王成章^1,2, 叶建中¹, 李文君¹, 鲁黎³

1. 中国林业科学研究院林产化学工业研究所;生物质化学利用国家工程实验室;国家林业局林产化学工程重点开放性实验室;江苏省生物质能源与材料重点实验室, 江苏南京 210042;
2. 中国林业科学研究院林业新技术研究所, 北京 1000913;
3. 湖北省林业科学研究院, 湖北武汉 430079

收稿日期:2014-01-18 出版日期:2014-10-25 发布日期:2015-08-18
作者简介:闵凡芹（1987-），女，山东临沂人，硕士生，研究方向：天然产物研究与开发
基金资助:
国家863计划资助（2014AA021802）；国际科技合作专项（2014DFR31300）

Screening of Tannic Acid Degrading Strain and Optimization of Its Fermentation Conditions

MIN Fan-qin¹, WANG Cheng-zhang^1,2, YE Jian-zhong¹, LI Wen-jun¹, LU Li³

1. Institute of Chemical Industry of Forest Products, CAF;National Engineering Lab.for Biomass Chemical Utilization;Key and Open Lab.of Forest Chemical Engineering, SFA;Key Lab. of Biomass Energy and Material, Jiangsu Province, Nanjing 210042, China;
2. Research Institute of Forestry New Technology, CAF, Beijing 100091, China;
3. Hubei Academy of Forestry, Wuhan 430079, China

Received:2014-01-18 Online:2014-10-25 Published:2015-08-18
Contact: 王成章（1966-），男，湖北汉川人，研究员，博士生导师，主要从事天然产物研究与利用；E-mail：wangc_z@163.com E-mail:wangc_z@163.com

摘要/Abstract

摘要： 通过平板涂布、划线分离、菌落观察等方法，从土耳其倍子提取液自然生长的菌落中分离纯化出11株菌，从中初筛出1^#、2^#、4^#这3株降解没食子单宁的菌株，复筛出1株没食子单宁优良降解菌株1^#菌，其没食子单宁降解率达65 %以上，发酵液中没食子酸质量浓度为0.052 3 g/L，单宁酶活为0.920 U/mL；对1^#菌株的发酵条件进行了响应面法优化，结果表明： 1^#菌最佳产酶条件为培养温度31 ℃，初始培养基pH值5.0，最适培养时间50 h，在该条件下1^#菌单宁酶酶活可达1.170 U/mL，与优化前的最大酶活0.920 U/mL相比，提高了27.2 %。

关键词: 没食子单宁, 优良降解菌株, 筛选, 发酵条件优化

Abstract: 3 strains of tannic acid degrading were screened out from the 11 strains which were separated from naturally fermented Turkish galls extract by the methods of spread-plate, streaking and colony observing,etc. One superior tannic acid degrading strain was found, named strain 1^#. The degradation rate of tannic acid reached beyond 65 %. In the fermentation broth, the concentration of gallic acid was 0.052 3 g/mL and the tannase activity was 0.920 U/mL. Fermentation conditions of strain 1^# were optimized by using Response Surface Method. The effect of temperature, initial pH, incubation time was investigated. The results showed that the optimum temperature, initial pH values and incubation time of strain 1^# was 31 ℃, 5 and 50 h. Under this condition, the tannase activity was increased from 0.920 to 1.170 U/mL, and improved by 27.2%.

Key words: tannic acid of Turkish galls, superior degrading strains, screening, optimization of fermentation conditions

中图分类号:

TQ35

闵凡芹, 王成章, 叶建中, 李文君, 鲁黎. 没食子单宁降解菌株的筛选及发酵条件优化[J]. 林产化学与工业, 2014, 34(5): 84-90.

MIN Fan-qin, WANG Cheng-zhang, YE Jian-zhong, LI Wen-jun, LU Li. Screening of Tannic Acid Degrading Strain and Optimization of Its Fermentation Conditions[J]. Chemistry and Industry of Forest Products, 2014, 34(5): 84-90.

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没食子单宁降解菌株的筛选及发酵条件优化

Screening of Tannic Acid Degrading Strain and Optimization of Its Fermentation Conditions

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