漆树多酚的负压提取工艺及其抑制酪氨酸酶活性的研究

doi:10.3969/j.issn.0253-2417.2015.02.007

林产化学与工业 ›› 2015, Vol. 35 ›› Issue (2): 47-54.doi: 10.3969/j.issn.0253-2417.2015.02.007

漆树多酚的负压提取工艺及其抑制酪氨酸酶活性的研究

陈虹霞^1,2, 王成章^1,2, 叶建中², 周昊^1,2, 张宇思², 陶冉²

1. 中国林业科学研究院林业新技术研究所, 北京 100091;
2. 中国林业科学研究院林产化学工业研究所;生物质化学利用国家工程实验室;国家林业局林产化学工程重点开放性实验室; 江苏省生物质能源与材料重点实验室, 江苏南京 210042

收稿日期:2014-09-03 出版日期:2015-04-25 发布日期:2015-04-24
通讯作者: 王成章(1966-),男,湖北汉川人,研究员,博士,博士生导师,主要从事天然产物研究与利用;E-mail:wangczlhs@sina.com。 E-mail:wangczlhs@sina.com
作者简介:陈虹霞(1983-),女,浙江舟山人,助理研究员,博士生,从事天然产物化学研究;E-mail:shirenyahui@126.com
基金资助:
中国林科院林业新技术所基本科研业务费专项资金(CAFINT2013C03);国家国际科技合作专项(2014DFR31300)

Phenolic Extracts from Rhus verniciflua Stokes Woods by Vacuum Extraction and Their Anti-tyrosinase Activities

CHEN Hong-xia^1,2, WANG Cheng-zhang^1,2, YE Jian-zhong², ZHOU Hao^1,2, ZHANG Yu-si², TAO Ran²

1. Research Institute of Forestry New Technology, CAF, Beijing 100091, China;
2. Institute of Chemical Industry of Forest Products, CAF;National Engineering Lab.for Biomass Chemical Utilization;Key and Open Lab. of Forest Chemical Engineering, SFA;Key Lab.of Biomass Energy and Material, Jiangsu Province, Nanjing 210042, China

Received:2014-09-03 Online:2015-04-25 Published:2015-04-24

摘要/Abstract

摘要： 采用负压提取工艺从漆树中提取漆树多酚,并研究其对酪氨酸酶活性的抑制作用。通过单因素和响应曲面试验优化负压提取漆树多酚的最佳工艺条件为真空度0.082 MPa,乙醇体积分数83%,时间1.5 h,液料比20:1(mL:g),提取次数2次,漆树多酚得率为2.193%。通过溶剂逐级萃取漆树多酚,得到含漆树多酚53.43%的乙酸乙酯萃取物,其对酪氨酸酶具有较强作用,抑制率为63.4%,相对抑制率50%的乙酸乙酯萃取物质量浓度为308 mg/L。对酪氨酸酶单酚酶的抑制效应表现为迟滞效应,500 mg/L乙酸乙酯萃取物能将反应初速度由29 μmol/(L·min)降至8 μmol·(L·min)。通过Lineweaver-Burk图显示对二酚酶的抑制作用表现为混合型抑制。

关键词: 漆树, 多酚, 负压提取, 酪氨酸酶, 抑制率

Abstract: The polyphenols were extracted from Rhus verniciflua Stokes by vacuum extraction, and the anti-tyrosinase activities were studied. The optimal conditions were investigated through single factor and response surface method. The optimal parameters were vacuum degree 0.082 MPa, ethanol volume fraction 83%, extraction time 1.5 h, liquid-solid ratio 20:1(mL:g), and extracted twice. At these conditions, the yield of polyphenol was 2.193%. Though the extraction, the ethyl acetate extract with 53.43% of phenolic was obtained and the inhibitory rate of tyrosinase was 63.4%. The ethyl acetate extract concentration leading to 50% inhibitory rate was 308 mg/L. The ethyl acetate extract could extend the lag time of tyrosine monophenolase, and 500 mg/L of extract could decrease the velocity from 29 μmol/(L·min) to 8 μmol/(L·min). The ethyl acetate extract functioned as a hybrid inhibition of tyrosine diphenolase by Lineweaver-Burk plots.

Key words: Rhus verniciflua Stokes, polyphenol, vacuum extraction, tyrosinase, inhibition rate

中图分类号:

TQ35

陈虹霞, 王成章, 叶建中, 周昊, 张宇思, 陶冉. 漆树多酚的负压提取工艺及其抑制酪氨酸酶活性的研究[J]. 林产化学与工业, 2015, 35(2): 47-54.

CHEN Hong-xia, WANG Cheng-zhang, YE Jian-zhong, ZHOU Hao, ZHANG Yu-si, TAO Ran. Phenolic Extracts from Rhus verniciflua Stokes Woods by Vacuum Extraction and Their Anti-tyrosinase Activities[J]. Chemistry and Industry of Forest Products, 2015, 35(2): 47-54.

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漆树多酚的负压提取工艺及其抑制酪氨酸酶活性的研究

Phenolic Extracts from Rhus verniciflua Stokes Woods by Vacuum Extraction and Their Anti-tyrosinase Activities

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