离子液体[Hmim][CF3SO3]在木质素及其模型化合物生物降解中的应用

doi:10.3969/j.issn.0253-2417.2016.04.001

林产化学与工业 ›› 2016, Vol. 36 ›› Issue (4): 1-6.doi: 10.3969/j.issn.0253-2417.2016.04.001

• 研究报告 • 下一篇

离子液体[Hmim][CF₃SO₃]在木质素及其模型化合物生物降解中的应用

崔晶晶¹, 李云华², 陈秉辉^1,2

1. 厦门大学能源学院, 福建厦门 361005;
2. 厦门大学化学化工学院, 福建厦门 361005

收稿日期:2015-04-17 出版日期:2016-08-25 发布日期:2016-09-01
通讯作者: 陈秉辉,教授,博士生导师,研究领域为催化与反应工程;E-mail:chenbh@xmu.edu.cn。 E-mail:chenbh@xmu.edu.cn
作者简介:崔晶晶(1983-),女,湖南湘潭人,博士生,主要从事生物质高附加值转化的研究工作
基金资助:
国家973计划资助（2010CB732200）

Application of Ionic Liquid [Hmim] [CF₃SO₃] in Biodegradation of Lignin & Lignin Model Compounds

CUI Jing-jing¹, LI Yun-hua², CHEN Bing-hui^1,2

1. School of Energy, Xiamen University, Xiamen 361005, China;
2. School of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005, China

Received:2015-04-17 Online:2016-08-25 Published:2016-09-01

摘要/Abstract

摘要： 研究了在室温下，离子液体[Hmim] [CF₃SO₃] 溶解木质素及其模型化合物后，与酶液在小分子有机酸作用下形成的三元液相平衡体系，及在此体系中进行酶促反应的过程。结果表明：小分子有机酸使水-离子液体形成的两相转化为均相的主要贡献是由羧基提供的，[Hmim] [CF₃SO₃] 在溶解木质素后依旧保留与有机酸和水形成三元相平衡体系的能力；以酶液作为水相，以溶解了木质素的离子液体作为疏水相，甲酸的掺入在起到平衡水相和疏水相的同时也作为木质素降解酶反应过程中的自由基稳定剂；在三元液相体系的反应中，离子液体引起酶的部分失活；随着反应的进行，碱木质素和木质素脱氢聚合物（DHPs）的降解率分别为56.5%和66.5%，甲氧基分别降低了46.7%和45.3%，生成的酚类化合物分别为204.7和207.0 mg/L，甲酸分别消耗了0.57和0.76 g。DHPs在降解过程中，由于甲酸的过度消耗导致了平衡体系的解体。

关键词: 三元液相体系, 离子液体, 木质素降解, 酚

Abstract: The ternary liquid-liquid equilibrium system composed of ionic liquid [Hmim] [CF₃SO₃] which dissolved lignin with enzyme solution and the organic acid was investigated in present study. The enzymatic reactions in the system were also studied. The transformation from water-ionic two phase system to homogeneous system should be attributed to the function of carboxylic acid groups in small molecule organic acids. [Hmim] [CF₃SO₃] with dissolved lignin could also compose the ternary system with water and organic acid. In the degradation reaction, formic acid played two roles in balancing the hydrophobic phase ([Hmim] [CF₃SO₃] with dissolved lignin), and aqueous phase (enzyme solution), and acting as radical stabilizer for lignin degrading enzymatic reaction. The degradation rates of alkali lignin and dehydrogenation polymers of lignin (DHPs) were 56.5% and 66.5%, and the content of methoxyl group decreased by 46.7% and 45.3%, respectively. The generated phenolic compounds were 204.7 and 207.0 mg/L, and the formic acid consumption were 0.57 and 0.76 g, respectively. In the DHPs degradation process, the ternary equilibrium was disintegrated due to the excessive consumption of formic acid.

Key words: ternary liquid-liquid system, ionic liquid, lignin degradation, phenols

中图分类号:

TQ351

崔晶晶, 李云华, 陈秉辉. 离子液体[Hmim][CF₃SO₃]在木质素及其模型化合物生物降解中的应用[J]. 林产化学与工业, 2016, 36(4): 1-6.

CUI Jing-jing, LI Yun-hua, CHEN Bing-hui. Application of Ionic Liquid [Hmim] [CF₃SO₃] in Biodegradation of Lignin & Lignin Model Compounds[J]. Chemistry and Industry of Forest Products, 2016, 36(4): 1-6.

参考文献

[1] HOLLDAY J E,WHITE J F,BOZELL J J,et al.Top Value-added Chemicals from Biomass.Volume II:Results of Screening for Potential Candidates from Biorefinery Lignin[M].Richland,WA:Pacific Northwest National Laboratory,2007:39.
[2] KAMM B,KAMM M.Biorefineries:Multi product processes [J].Advances in Biochemical Engineering/Biotechnology,2007,105:175-204.
[3] SAISU M,SATO T,WATANABE M,et al.Conversion of lignin with supercritical water-phenol mixtures [J].Energy & Fuels,2003,17(4):922-928.
[4] PU Yun-qiao,JIANG Nan,RAGAUSKAS A J.Ionic liquid as a green solvent for lignin[J].Journal of Wood Chemistry and Technology,2007,27(1):23-33.
[5] HA S H,KOO Y M.Enzyme performance in ionic liquids [J].Korean Journal of Chemical Engineering,2011,28(11):2095-2101.
[6] CARVALHEIRO F,DUARTE L C,GIRIO F M.Hemicellulose biorefineries:A review on biomass pretreatments [J].Journal of Scientific & Industrial Research,2008,67(11):849-864.
[7] MOURÃO T,CLÁUDIO A F M,BOAL-PALHEIROS I,et al.Evaluation of the impact of phosphate salts on the formation of ionic-liquid-based aqueous biphasic systems[J].The Journal of Chemical Thermodynamics,2012,54:389-405.
[8] HUDDLESTON J G,WILLAUER H D,SWATLOSKI R P,et al.Room temperature ionic liquids as novel media for ‘clean’ liquid-liquid extraction [J].Chemical Communications,1998,16:1765-1766.
[9] SARKANEN K V,LUDMIG C H.Lignin Occurrence,Formation,Structure and Reactions [M].New York Wiley-Interscience,1971:149-151.
[10] BROWN M E,CHANG M C.Exploring bacterial lignin degradation[J].Current Opinion in Chemical Biology,2014,19:1-7.
[11] 李慧蓉.白腐真菌生物学和生物技术[M].北京:化学工业出版社,2005:57. LI Hui-rong.White-rot Fungi Biology and Biotechnology [M].Beijing:Chemical Industry Press,2005:57.
[12] GOLD M H,GLENN J K.Manganese peroxidase from Phanerochaete chrysosporium[J].Methods in Enzymology,1988,161:258-264.
[13] CHAKRABORTY T K,DAS N,SENGUPTA S,et al.Accumulation of a natural substrate of laccase in gills of Pleurotus florida during sporulation[J].Current Microbiology,2000,41(3):167-171.
[14] 罗亚楠,于丽颖,刘立群.绿茶中茶多酚的提取及分析检测[J]. 安徽农业科学,2011,39(26):16429-16430. LUO Ya-nan ,YU Li-ying,LIU Li-qun.Extraction of tea polyphenol in green tea and its analysis detecting [J].Journal of Anhui Agricultural Sciences, 2011,39(26):16429-16430.
[15] 陈云平,陈婷,陈为健.高沸醇木质素甲氧基含量测定[J].闽江学院学报,2005,25(2):66-70. CHEN Yun-ping,CHEN Ting,CHEN Wei-jian.Determination of methoxyl group in high boiling solvent lignin [J].Journal of Minjiang University,2005,25(2):66-70.

离子液体[Hmim][CF₃SO₃]在木质素及其模型化合物生物降解中的应用

Application of Ionic Liquid [Hmim] [CF₃SO₃] in Biodegradation of Lignin & Lignin Model Compounds

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