氧浓度对固定化黄孢原毛平革菌合成过氧化物酶的影响

林产化学与工业 ›› 2000, Vol. 20 ›› Issue (3): 40-46.

氧浓度对固定化黄孢原毛平革菌合成过氧化物酶的影响

柯世省¹, 夏黎明², 张朝晖³, 岑沛霖²

1. 浙江省台州师范专科学校生物系, 浙江临海 317000;2. 浙江大学化工学院生物化工系, 浙江杭州 310027;3. 浙江工业大学生物和环境工程学院, 浙江杭州 310032

收稿日期:1999-07-09 修回日期:1900-01-01 出版日期:2000-09-30 发布日期:2000-09-30

INFLUENCE OF OXYGEN CONCENTRATION ON THE PRODUCTION OF PEROXIDASES BY PHANEROCHAETE CHRYSOSPORIUM

KE Shi-sheng¹, XIA Li-ming², ZHANG Zhao-hui³, CEN Pei-lin²

1. Department of Biology, Taizhou Teacher's College, Linhai 317000, China;2. Department of Biochemical Engineering, Zhejiang University, Hangzhou 310027, China;3. Institute of Biology and Environment and Engineering, Zhejiang Industrial Univeristy, Hangzhou 310032, China

Received:1999-07-09 Revised:1900-01-01 Online:2000-09-30 Published:2000-09-30

摘要/Abstract

摘要： 氧浓度对固定化黄孢原毛平革菌 (Phanerochaete chrysosporium)合成过氧化物酶有较大的影响。限碳条件下培养黄孢原毛平革菌,生长阶段氧浓度对合成木质素过氧化物酶的影响不明显,但在产酶阶段氧浓度的影响却很大。 21%氧浓度即空气环境是合成木质素过氧化物酶和锰过氧化物酶适宜的。增高氧浓度,胞外蛋白酶活力就会提高,不利于过氧化物酶的胞外累积。研究结果表明,空气环境下限碳培养黄孢原毛平革菌,合成的木质素过氧化物酶最高活力可达 360U/L,比通纯氧条件下限碳培养时的木质素过氧化物酶要高 160%。

关键词: 固定化黄孢原毛平革菌, 木质素过氧化物酶, 锰过氧化物酶, 氧浓度限碳培养

Abstract: Oxygen concentration had obvious influences on the production of peroxidases by Phanerochaete chrysosporium. Under limited carbon source culture condition, oxygen concentration in the growth period had little influence on the production of lignin peroxidase, but it had obvious influences in the second metabolism period in which peroxidases were produced.It was found that 21% O₂(in air)was the optimal oxygen condition on production of peroxidases (including both lignin peroxidase and Mn-dependent peroxidase).Further increase of oxygen concentration depressed the production of peroxidases, because more extracellular protease which was harmful to peroxidases would be produced.At the optimal oxygen concentration (in air 21% O₂), the maximal lignin peroxidase activity was 360U/L under limited carbon source culture condition.

Key words: immobilized Phanerochaete chrysosporium, lignin peroxidase, Mn-dependent peroxidase

中图分类号:

Q554⁺.6

柯世省;夏黎明;张朝晖;岑沛霖. 氧浓度对固定化黄孢原毛平革菌合成过氧化物酶的影响[J]. 林产化学与工业, 2000, 20(3): 40-46.

KE Shi-sheng;XIA Li-ming;ZHANG Zhao-hui;CEN Pei-lin. INFLUENCE OF OXYGEN CONCENTRATION ON THE PRODUCTION OF PEROXIDASES BY PHANEROCHAETE CHRYSOSPORIUM[J]. , 2000, 20(3): 40-46.

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[2]	谢慧芳;近藤隆一郎;李忠正. 白腐菌Phanerochaete sordida YK-624产锰过氧化物酶的生产及初步纯化[J]. 林产化学与工业, 2003, 23(04): 22-26.