产焦性没食子酸菌株筛选及其培养条件优化

doi:10.3969/j.issn.0253-2417.2018.03.013

林产化学与工业 ›› 2018, Vol. 38 ›› Issue (3): 97-102.doi: 10.3969/j.issn.0253-2417.2018.03.013

产焦性没食子酸菌株筛选及其培养条件优化

徐曼, 汪咏梅, 张亮亮

中国林业科学研究院林产化学工业研究所;生物质化学利用国家工程实验室;国家林业局林产化学工程重点开放性实验室;江苏省生物质能源与材料重点实验室, 江苏南京 210042

收稿日期:2017-12-14 出版日期:2018-06-25 发布日期:2018-06-22
通讯作者: 汪咏梅,副研究员,主要从事植物资源化学利用研究;E-mail:njlhswym@163.com。 E-mail:njlhswym@163.com
作者简介:徐曼(1981-),女,江苏沭阳人,助理研究员,硕士,主要从事植物资源化学利用研究;E-mail:lhsxuman@163.com
基金资助:
国家863计划资助项目（SS2014AA021802）

Screening and Optimization of Culture Condition of a Pyrogallol-producing Strain

XU Man, WANG Yongmei, ZHANG Liangliang

Institute of Chemical Industry of Forest Products, CAF;National Engineering Lab. for Biomass Chemical Utilization;Key and Open Lab. of Forest Chemical Engineering, SFA;Key Lab. of Biomass Energy and Material, Jiangsu Province, Nanjing 210042, China

Received:2017-12-14 Online:2018-06-25 Published:2018-06-22

摘要/Abstract

摘要： 研究了肠杆菌、短乳杆菌和植物乳杆菌3种菌种催化底物没食子酸降解产焦性没食子酸的能力，其中产焦性没食子酸效果较好的是肠杆菌和植物乳杆菌。对不同温度、底物浓度、pH值、离子浓度等反应条件对肠杆菌和植物乳杆菌没食子酸脱羧酶酶活的影响进行分析，并确定了这两株菌降解没食子酸的培养条件。结果表明：在培养温度为28℃、没食子酸浓度为30 mmol/L，（NH₄）₂SO₄浓度8 mmol/L，MgSO₄浓度为80 mmol/L，初始培养基pH值6.0，培养48 h时，肠杆菌催化没食子酸产焦性没食子酸58.11%；在培养温度为37℃、底物浓度为10 mmol/L，（NH₄）₂SO₄浓度为8 mmol/L，MgSO₄浓度为20 mmol/L，初始培养基pH值6.5，培养48 h时，植物乳杆菌催化没食子酸产焦性没食子酸83.31%。

关键词: 没食子酸, 焦性没食子酸, 没食子酸脱羧酶, 肠杆菌, 植物乳杆菌

Abstract: Three bacteria strains including Enterobacter spp., Lactobacillus brevis and Lactobacillus plantarum were tested for degrading gallic acid into pyrogallol. The factors affecting the activity of gallate decarboxylase from E. spp. and L. plantarum, including substrate concentration, temperature, pH, magnesium sulfate (MgSO₄) concentration were also studied. The experiment results showed that the yield of pyrogallol by E. spp. could reach 58.11% with the temperature 28℃, 30 mmol/L gallic acid, 8 mmol/L (NH₄)₂SO₄, 80 mmol/L MgSO₄ and pH 6.0 after cultured for 48 h. Meanwhile, the yield of pyrogallol by L. plantarum could reach 83.31% with the temperature 37℃, substrate concentration 10 mmol/L, concentration of (NH₄)₂SO₄ 8 mmol/L, concentration of MgSO₄ 20 mmol/L and pH 6.5 after cultured for 48 h.

Key words: gallic acid, pyrogallol, gallic acid decarboxylase, Enterobacter spp., Lactobacillus plantarum

中图分类号:

TQ35

徐曼, 汪咏梅, 张亮亮. 产焦性没食子酸菌株筛选及其培养条件优化[J]. 林产化学与工业, 2018, 38(3): 97-102.

XU Man, WANG Yongmei, ZHANG Liangliang. Screening and Optimization of Culture Condition of a Pyrogallol-producing Strain[J]. Chemistry and Industry of Forest Products, 2018, 38(3): 97-102.

参考文献

[1] 谢兵,蔡小华,黄鹏飞. 多羟基二苯甲酮的研究进展[J]. 应用化工,2008,37(9):1086-1088. XIE B,CAI X H,HUANG P F. Research advances of polyhydroxyben zophenone[J]. Applied Chemical Industry,2008,37(9):1086-1088.
[2] 孙玉敏,宫婷,梁玉珍. 焦性没食子酸树脂相光度法测定痕量钒(V)[J]. 理化检测:化学分册,2006,42(8):649-653. SUN Y M,GONG T,LIANG Y Z. Resin-phase photometric determination of Vanadium (V) with pypogallic acid as color reagent[J]. PTCA (Part B:Chemical Analysis),2006,42(8):649-650,653.
[3] 梁海燕,李金培,马望京. 一种新型热成像材料[J]. 影像技术,2007(2):11-12. LIANG H Y,LI J P,MA W J. A new theromographic material[J]. Image Technology,2007(2):11-12.
[4] 杜运平,张宗和. 焦性没食子酸的制备方法及应用[J]. 生物质化学工程,2011,45(1):47-52. DU Y P,ZHANG Z H. Preparation and application of pyrogallol[J]. Biomass Chemical Engineering,2011,45(1):47-52.
[5] 张亮亮,汪咏梅,徐曼,等. 没食子酸脱羧酶及酶法制备焦性没食子酸研究进展[J]. 生物质化学工程,2014,48(4):35-39. ZHANG L L,WANG Y M,XU M,et al. Research progress on gallic acid decarboxylase and microbial production of pyrogallol through decarboxylation of gallic acid[J]. Biomass Chemical Engineering,2014,48(4):35-39.
[6] ZEIDA M,WIESER M,YOSHIDA T,et al. Purification and characterization of gallic acid decarboxylase from Pantoea agglomerans T71[J]. Applied and Environmental Microbiology,1998,64(12):4743-4747.
[7] BRUNE A,SCHINK B. Phloroglucinol pathway in the strictly anaerobic Pelobacter acidigallici:Fermentation of trihydroxybenzenes to acetate via triacetic acid[J]. Archives of Microbiology,1992,157(5):417-424.
[8] SONI M,SHARMA K P,JOHN P. Characterization of pyrogallol production from gallic acid by Enterobacter spp.[J]. Journal of Microbiology and Biotechnology Research,2012,2(2):327-336.
[9] YOSHIDA H,YAMADA H. Microbial production of pyrogallol through decarboxylation of gallic acid[J]. Agricultural and Biological Chemistry,1985,49(3):659-663.
[10] ODENYO A A,BIOHOP R,ASEFA G,et al. Characterization of tannin-tolerant bacterial isolates from East African ruminants[J]. Anaerobe,2001,7(1):5-15.
[11] O'DONNVAN L,BROOHER J D. Effect of hydrolysable and condensed tannins on growth,morphology and metabolism of Streptococcus gallolyticus (S. caprinus) and Streptococcus bovis[J]. Microbiology,2001,147(4):1025-1033.
[12] BRADFORD M M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of proteindye binding[J]. Analytical Biochemistry,1976,72(1/2):248-254.
[13] 李文君,王成章,闵凡芹,等. 降解没食子酸制备焦性没食子酸的菌种筛选及工艺条件[J]. 精细化工,2015,32(1):52-58. LI W J,WANG C Z,MIN F Q,et al. Screening strains and conditions for the degradation of gallic acid to prepare pyrogallol[J]. Fine Chemicals,2015,32(1):52-58.
[14] JIMEMEZ N,CUIEL J A,REVERON I,et al. Uncovering the Lactobacillus plantarum WCFS1 gallate decarboxylase involved in tannin degradation[J]. Applied and Environmental Microbiology,2013,79(14):4253-4263.

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产焦性没食子酸菌株筛选及其培养条件优化

Screening and Optimization of Culture Condition of a Pyrogallol-producing Strain

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