杨木心材白腐菌预处理及生物化机浆制浆性能研究

doi:10.3969/j.issn.0253-2417.2022.06.013

摘要/Abstract

摘要：

利用白腐菌Trametes sp.48424预处理杨木心材, 然后进行生物化学机械法制浆，测定了白腐菌预处理杨木心材木片时的漆酶酶活, 并分析了预处理前后杨木心材的化学组分的变化。利用扫描电镜(SEM)、红外光谱(FT-IR)、核磁共振碳谱(¹³C NMR)等深入研究了白腐菌对杨木心材的降解机理，同时利用化学机械法制浆, 探讨浆料纤维形态和成纸性能变化。研究结果表明：白腐菌能很好地在杨木心材木片上进行固态发酵, 并在预处理第8天时漆酶酶活达到最大值1.3 IU/mL。在预处理阶段, 白腐菌会使木片表面出现孔洞和凹槽, 并且对Klason木质素的降解速率高于对综纤维素的降解速率。白腐菌预处理对磨木木质素(MWL)中的β-O-4结构的侧链(δ 60.6和72.7)的降解较为明显, 而对芳香环的影响较小, 反映了白腐菌对木质素的降解主要发生在木质素侧链。与对照组相比, 杨木心材木片经过白腐菌预处理后, 纸浆的纤维长度、扭结指数增加, 细小纤维含量减少, 成纸强度显著提高。

关键词: 白腐菌, 杨木心材, 磨木木质素, 纸张强度

Abstract:

The white-rot fungus Trametes sp.48424 was used to pretreat poplar heartwood for the biochemical-mechanical pulp(Bio-CMP). The laccase activity of white-rot fungus during pretreatment was measured, and the changes in the chemical composition of poplar heartwood before and after pretreatment were also analyzed. Moreover, scanning electron microscopy(SEM), Fourier transform infrared spectroscopy(FT-IR), carbon-13 nuclear magnetic resonance(¹³C NMR) were applied to intensively study the degradation mechanism of peplar heartwood by white-rot-fungus. Using Bio-CMP process, the changes of pulp fiber morphology and paper properties were discussed. The results showed that the white-rot fungus could perform solid-state fermentation well on the surface of the heartwood chips, and the laccase activity reached the maximum value of 1.3 IU/mL after 8 day of pretreatment. In the pretreatment stage, the white-rot fungus could lead to a porous and wrinkled surface structrue of the wood chip, and the degradation rate of Klason lignin was higher than that of holocellulose. White-rot fungus pretreatment could cleave β-O-4 side chain(δ 60.6, 72.7) substructure in milled wood lignin(MWL) effectively, while it had little effect on aromatic rings, reflecting that the degradation of lignin by white-rot fungus mainly happened on the lignin side chain. Compared with reference experiment, after poplar heartwood chips being pretreated by white-rot fungus, the fiber length and kinking index of the pulp increased, the content of fines reduced, and the paper strength enhanced obviously.

Key words: white-rot fungus, poplar heartwood, milled wood lignin, paper strength

中图分类号:

TQ35
TS743

罗文琪, 谢益民, 张功侠. 杨木心材白腐菌预处理及生物化机浆制浆性能研究[J]. 林产化学与工业, 2022, 42(6): 91-98.

Wenqi LUO, Yimin XIE, Gongxia ZHANG. Pretreatment of Poplar Heartwood by White-rot Fungus and Performance of Biological Chemi-mechanical Pulping[J]. Chemistry and Industry of Forest Products, 2022, 42(6): 91-98.

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样品 samples	Klason木质素 Klason lignin	综纤维素 holocellulose	1% NaOH抽出物 1% NaOH extractive	苯-醇抽出物 benzene-alcohol extractive	丙酮抽出物 acetone extractive	热水抽出物 hot water extractive
心材heartwood	24.24	76.72	18.30	1.35	0.80	3.22
边材sapwood	21.46	79.21	15.66	1.21	0.65	1.68

浆料¹⁾ pulp	重均长度/mm weight average length	纤维宽度/μm fiber width	细小纤维/% fine	扭结指数 kink index
6%Bio-CMP	0.589	29.3	18.3	2.825
6%-CMP	0.571	29.7	22.9	2.664
8%Bio-CMP	0.665	28.1	14.0	3.047
8%-CMP	0.652	28.7	15.4	2.903
10%Bio-CMP	0.582	29.0	14.8	2.900
10%-CMP	0.576	29.6	19.2	2.733

浆料 pulp	紧度/(g·cm^-3) density	抗张指数/(N·m·g^-1) tensile index	环压指数/(N·m·g^-1) ring crush index	耐破指数/(kPa·m²·g^-1) bursting index	耐折次数/(MIT) folding endurance
6%Bio-CMP	0.515	35.2	8.6	1.67	1
6%-CMP	0.472	31.0	7.0	1.47	1
8%Bio-CMP	0.541	38.2	9.2	2.04	5
8%-CMP	0.519	32.9	7.9	1.82	3
10%Bio-CMP	0.505	35.3	8.2	1.90	4
10%-CMP	0.493	31.5	7.7	1.67	2

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