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林产化学与工业

林产化学与工业 ›› 2012, Vol. 32 ›› Issue (6): 89-93.

• 1研究报告 • 上一篇    下一篇

无患子叶皂苷提取及结构表征

高飞飞1,2, 赵扬1,2, 罗金岳1,2   

  1. 1. 南京林业大学化学工程学院, 江苏 南京 210037;2. 江苏省生物质绿色燃料与 化学品重点实验室, 江苏 南京 210037
  • 收稿日期:2011-11-18 修回日期:1900-01-01 出版日期:2013-12-30 发布日期:2013-12-30
  • 通讯作者: 罗金岳,男,教授,硕士生导师,主要研究方向为林产化学加工;E-mail:luojinyue@njfu.com.cn。

Extraction and Characterization of Saponins from Sapindus

GAO Fei-fei1,2, ZHAO Yang1,2, LUO Jin-yue1,2   

  1. 1. College of Chemical Engineering,Nanjing Forestry University, Nanjing 210037, China;2. Jiangsu Key Lab of Biomass-based Green Fuels and Chemicals, Nanjing 210037, China
  • Received:2011-11-18 Revised:1900-01-01 Online:2013-12-30 Published:2013-12-30

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摘要： 以无患子叶子为研究对象,分别考察了索氏抽提法与微波萃取法对皂苷提取得率的影响,确定了较优的提取工艺。结果表明,索氏抽提选择80%乙醇为提取剂,在液料比30∶1(g∶g,下同),提取温度95℃,抽提时间为3h,提取2次时皂苷元提取得率达到21.7%;微波萃取选择3g原料,微波功率300W,温度70℃,90%的乙醇,液料比20∶1,提取时间45min,皂苷的提取得率达到22.3%。目标提取物经FT-IR、HPLC和1HNMR表征,确定其结构为常春藤皂苷元。

关键词: 无患子, 皂苷, 提取, 表征

Abstract: Saponins have the functions of detoxification,eliminating phlegm and anticancer etc. It is widely used in the field of medical and daily chemical products. The saponins yields were investigated by soxhlet extraction and microwave extraction based on the leaves of Sapindus. The resonable extraction technique was obtained. It is concluded that the suitable conditions of soxhlet extraction were 80% ethanol as extractant, ratio of liquid to material 30∶1 (g∶g), extraction temperature 95℃, extraction time 3h×2. The extraction yield was 21.7%. The suitable conditions of microwave extraction were microwave power 300W, extraction temperature 70℃, 90% ethanol as extractant, ratio of liquid to material 20∶1, extraction time 45min. The extraction yield reached 22.3%. The chemical structure of target extract was identified by FT-IR, HPLC and 1HNMR.

Key words: sapindus, saponin, extraction, characterization

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