超声波强化溶剂提取姜味草中熊果酸和齐墩果酸

林产化学与工业 ›› 2009, Vol. 29 ›› Issue (3): 111-114.

超声波强化溶剂提取姜味草中熊果酸和齐墩果酸

李坤平¹, 潘天玲¹, 贲永光¹, 潘天鸣²

1. 广东药学院, 药科学院, 广东广州 510006;2. 贵州省黔西南布依族苗族自治州人民医院, 贵州兴义 562400

收稿日期:2008-09-01 修回日期:1900-01-01 出版日期:2009-06-30 发布日期:2009-06-30

Ultrasonic-assisted Solvent Extraction of Ursolic Acid and Oleanolic Acid from Micromeria biflora Benth.

LI Kun-ping¹, PAN Tian-ling¹, BI Yong-guang¹, PAN Tian-ming²

1. Department of Pharmacy, Guangdong Pharmaceutical University, Guangzhou 510006, China;2. People's Hospital of Buyi and Miao Autonomous Prefecture in Southwest Guizhou, Xingyi 562400, China

Received:2008-09-01 Revised:1900-01-01 Online:2009-06-30 Published:2009-06-30

摘要/Abstract

摘要： 以齐墩果酸和熊果酸得率为指标,对超声波强化溶剂提取姜味草中齐墩果酸和熊果酸工艺进行了研究,利用单因素试验确定提取溶剂,通过正交设计对提取工艺进行优化,并与溶剂回流提取法进行了比较。结果表明,以乙醇为溶剂,超声波强化提取的最佳条件是:体积分数 90% 乙醇,固液比1:15,功率为 360W,频率 40kHz 超声波提取2次,每次 20min,齐墩果酸和熊果酸总得率为 0.732%;回流提取工艺的最优参数是:固液比1:15, 90℃ 回流提取2次,每次 30min,齐墩果酸和熊果酸总得率为 0.706%。超声波强化溶剂提取姜味草中熊果酸和齐墩果酸较溶剂回流提取法工艺简单,效率高。

关键词: 姜味草, 齐墩果酸, 熊果酸, 超声波强化提取

Abstract: Ultrasonic-assisted solvent extraction of oleanolic acid and ursolic acid from Micromeria biflora Benth., was studied and compared with solvent reflux extraction. The solvent was selected based on single factor experiments and the extracting process was optimized by orthogonal test. Using ethanol as solvent, the best results can be achieved under the following conditions:90% (volume fraction)ethanol, solid-liquid ratio 1:15, ultrasonic power 360W at 40kHz, extracting for 2 times, each time 20min, total yield of oleanolic acid and ursolic acid was 0.732%; for solvent reflux extraction the optimal parameters were:solid-liquid ratio 1:15, extracting 2 times at 90℃, each time 30min, total yiled of oleanolic acid and ursolic acid was 0.706%. Ultrasonic-assisted solvent extraction can effectively extract oleanolic acid and ursolic acid from M. biflora with advantages of simple operation andhigh efficency.

Key words: Micromeria biflora Benth., oleanolic acid, ursolic acid, ultrasonic-assisted extraction

中图分类号:

李坤平;潘天玲;贲永光;潘天鸣. 超声波强化溶剂提取姜味草中熊果酸和齐墩果酸[J]. 林产化学与工业, 2009, 29(3): 111-114.

LI Kun-ping;PAN Tian-ling;BI Yong-guang;PAN Tian-ming. Ultrasonic-assisted Solvent Extraction of Ursolic Acid and Oleanolic Acid from Micromeria biflora Benth.[J]. , 2009, 29(3): 111-114.

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