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林产化学与工业

林产化学与工业 ›› 2002, Vol. 22 ›› Issue (3): 45-48.

• 研究报告 • 上一篇    下一篇

中国红豆杉叶浸膏中紫杉烷的分离纯化及成分鉴定

余龙江, 兰文智   

  1. 华中科技大学, 生命科学与技术学院, 湖北, 武汉, 430074
  • 收稿日期:2001-09-10 修回日期:1900-01-01 出版日期:2002-09-30 发布日期:2002-09-30

SEPARATION,PURIFICATION AND IDENTIFICATION OF TAXANES IN EXTRACT OF TAXUS CHINENSIS LEAVES

YU Long-jiang, LAN Wen-zhi   

  1. School of Life Science and Technology, Huazhong University of Science and Technology, Wuhan 430074, China
  • Received:2001-09-10 Revised:1900-01-01 Online:2002-09-30 Published:2002-09-30

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摘要： 以中国红豆杉[Taxus chinensis(Pilger)Rehd.]叶为原料,利用甲醇或乙醇为溶剂制备浸膏。甲醇浸膏中的浆果赤霉素Ⅲ和紫杉醇的含量高于乙醇浸膏。采用萃取和层析方法相结合的分离纯化工艺,从甲醇浸膏中获得10个化合物纯品。利用NMR,IR,MS和HPLC等方法对其成分进行结构鉴定,确定5个化合物分别为紫杉醇、1-乙酰基5,7,10-去乙酰基浆果赤霉素I、浆果赤霉素Ⅲ、浆果赤霉素VI和7,9,10,13-去乙酰基浆果赤霉素VI,HPLC检测它们的纯度相应为98.4%,98.7%,99.1%,99.65%和92.94%。分离的紫杉醇和浆果赤霉素Ⅲ得率分别为62.6%和57.6%。这些结果表明所建立的制备及分离纯化的工艺可用于红豆杉叶生产紫杉醇及其前体物。

关键词: 红豆杉叶, 浸膏, 紫杉烷, 分离纯化

Abstract: Extract of Taxus chinensis (Pilger)Rehd.leaves was prepared by methanol or ethanol solvent extraction.Contents of baccatin Ⅲ and taxol in methanol extract were higher than those in ethanol extract.From methanol extract,10 pure compounds were obtained through isolation and purification process combined with extraction and chromatographic analysis.Ingredients were structurally identified through methods of NMR,IR,MS and HPLC,and five compounds were ascertained as taxol,1-accetyl-5,7,10-deacetyl baccatin Ⅰ,baccatin Ⅲ,baccatin Ⅵ and 7,9,10,13-deacetyl baccatin Ⅵ of purity 98.4%,98.7%, 99.1%, 99.65%,92.94%, respectively.Moreover,extraction yield of taxol and baccatin Ⅲ reached 62.6% and 57.6%,respectively.Results showed that the established preparation and purification technique could be applied to produce taxol and its precursors from T. chinensis leaves.

Key words: Taxus chinensis (Pilger) Rehd. leaves, extracts, taxane, separation and purification

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