匀浆法提取喜树果和喜树叶中喜树碱的研究

林产化学与工业 ›› 2009, Vol. 29 ›› Issue (1): 79-82.

匀浆法提取喜树果和喜树叶中喜树碱的研究

史伟国, 祖元刚, 赵春建, 杨磊, 李佳慧

东北林业大学森林植物生态学教育部重点实验室, 黑龙江哈尔滨 150040

收稿日期:2008-05-21 修回日期:1900-01-01 出版日期:2009-02-28 发布日期:2009-02-28
通讯作者: 祖元刚,教授,博士生导师,主要从事植物药基础研究与新药开发;E-mail:zygorl@vip.hl.cn。

Homogenated Extraction of Camptothecine from Camptotheca acuminata Decne. Seeds and Leaves

SHI Wei-guo, ZU Yuan-gang, ZHAO Chun-jian, YANG Lei, LI Jia-hui

Key Laboratory of Forest Plant Ecology, Ministry of Education, Northeast Forestry University, Harbin 150040, China

Received:2008-05-21 Revised:1900-01-01 Online:2009-02-28 Published:2009-02-28

摘要/Abstract

摘要： 以喜树果和喜树叶为原料,对匀浆法提取喜树碱的工艺进行了研究,并采用HPLC 法进行定量分析。确定了最佳的工艺条件:提取溶剂为体积分数55%的乙醇,匀浆时间为8min,料液比为1:15(g:mL)。在此工艺条件下,喜树果和喜树叶中喜树碱的得率分别为0.0801%和0.0679%。将该法与超声波提取、回流提取、常温冷浸提取、水浴振荡提取等方法进行了比较。结果表明,匀浆提取具有得率高、时间省等优势,是一种高效提取喜树碱的方法。

关键词: 匀浆法, 喜树果, 喜树叶, 喜树碱

Abstract: The method of homogenated extraction and HPLC determination of camptothecine in seed and leaf of common campto-theca(Camptotheca acuminata) were studied,and comparisons of this extraction method with other methods such as ultra sonic extraction,refluxing, soaking and shaking in water bath were conducted.For the homogenated extraction,the optimum technology parameters were found as following: homogenation for 8 min with 55% ethanol at a ratio of material to liquid of 1:15 (g:mL).By using the optimized parameters,the extraction yields of camptothecine from C. acuminata seeds and leaves were 0.0801% and 0.0679%,respectively.Moreover,homogenated extraction required less extraction time and had high yield,which was an efficient method for extracting camptothecine.

Key words: homogenation, Camptotheca acuminata seed, C. acuminata leaf, camptothecine

中图分类号:

史伟国;祖元刚;赵春建;杨磊;李佳慧. 匀浆法提取喜树果和喜树叶中喜树碱的研究[J]. 林产化学与工业, 2009, 29(1): 79-82.

SHI Wei-guo;ZU Yuan-gang;ZHAO Chun-jian;YANG Lei;LI Jia-hui. Homogenated Extraction of Camptothecine from Camptotheca acuminata Decne. Seeds and Leaves[J]. , 2009, 29(1): 79-82.