Abstract: Twenty four kinds of activated biocharcoal from different trimmed trees were prepared under hetero-thermal program. The yield, pH, ethylene blue adsorptive value (ab. EBAV), water absorption and water retention capacity were measured. Testments of antimicrobial effect of pyrolysated frozen liquid were conducted by controlling streptomycin. It was found that the hightest yield rate of biochar was from Cedar (29.8%), and the other starting materials gave almost the same yield from 10% to 15%. The activated biochar made from Eucommia ulmoides performed the highest ethylene blue discoloring ability (192.0 mg/g). The lowest and the highest pH biochar was represented from Prunus pit (7.94) and Elder (10.00), respectively. The pH of all biochars was higher than 7.0. The biochar from Chinese pine had the best water retention capacity after heating for 3 hours at 45-50℃. It can reserve 0.78 g water by each gram of biochar. It was also found that with the EBAV increased, the yield biochar decreased at the same time, whereas, the capacities of water absorption and water retention increased. However, several kinds of biochars from the same families or genuses tree show a little difference in adsorption value and pH. The antimicrobial experiments using streptomycin indicated all pyrolysates have antimicrobial activity, which gives better performance from the pyrolytic product at 400-600℃. However, the antimicrobial activity from the samples showed less durability compared with the effect of streptomycin (10 mg/L).