刺玫果提取物中总三萜酸的定性定量分析研究

doi:10.3969/j.issn.0253-2417.2015.05.022

Abstract

Abstract: The qualitative and quantitative analysis methods of total triterpenoid acids (TTA) in fruit extracts of Rosa davurica Pall. were established. Both UV and HPLC methods were applied to determine the content of TTA, oleanolic acid and ursolic acid. TLC method was used to accomplish the qualitative identification of ursolic acid in fruit extracts. The concentration of oleanolic acid showed good linear relationship with absorbance at the range of 1.75-15.75 mg/L (r=0.999 1) and the average recovery was 101.60% (RSD 1.23%) in quantitative analysis of TTA with UV method. On the other hand, oleanolic acid and ursolic acid showed good linear relationship at the range of 0.1-1.1 μg (r=0.999 7) and 0.024-0.768 μg (r=0.999 9) respectively and the average recoveries of them were 100.24%(RSD 1.44%) and 100.54%(RSD 0.45%) by HPLC method. The average contents of TTA, oleanolic acid, and ursolic acid of three batches of fruit extracts were 8.62%, 0.273%, and 0.534%, respectively. The experimental results showed that the content determination and TLC qualitative identification methods established could be applied to control of the qualitative and quantitative analysis of fruit extracts of R.davurica.

Key words: fruit of Rosa davurica Pall., total triterpenoid acids, oleanolic acid, ursolic acid

CLC Number:

TQ35

ZHONG Fang-li, WANG Xiao-lin, YANG Yang. Qualitative and Quantitative Analysis of Total Triterpenoid Acids in Fruit Extracts of Rosa davurica Pall.[J]. Chemistry and Industry of Forest Products, 2015, 35(5): 135-142.

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Qualitative and Quantitative Analysis of Total Triterpenoid Acids in Fruit Extracts of Rosa davurica Pall.

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