Abstract: Xylobiose and xylotriose were prepared from xylooligosaccharides by gel permeation chromatography,and their in vitro cultivation properties by Bifidobacterium adolescentis were investigated.Xylobiose and xylotriose were separated on polyacrylamide gel Bio-GelP-2 by FPLC system with degased high-purified water as eluant,and their (biological) characteristics toward bifidobacteria were studied.The results showed that the cell-dry-weights of B.adolescentis were 0.16,0.33 and 0.47 g/L,respectively,when xylose,xylobiose and xylotriose were used as carbon souces after 48 h cultivation in vitro.Xylotriose had superior effect than xylobiose and xylose on proliferation of B.adolescentis,and xylobiose had better effects than xylose.Xylobiose and xylotriose can reduce pH values of medium from 7.0 to 4.40 and 4.42,respectively.

Key words: xylooligosaccharides, xylobiose, xylotriose, gel permeation chromatography, bifidobacteria