Welcome to Chemistry and Industry of Forest Products,

›› 2008, Vol. 28 ›› Issue (6): 34-38.

• 研究报告 • Previous Articles     Next Articles

国家自然科学基金(30770231); 林业科技支撑计划子课题(2006BAD18B0405); 教育部科学技术研究重点项目(108049); 哈尔滨市科技创新人才研究专项资金(2006RFXXS001)

TONG Mei-hong, LIU Wei, FU Yu-jie, KONG Yu, GU Cheng-bo, ZHANG Lin   

  1. Key Laboratory of Forest Plant Ecology, Ministry of Education, Northeast Forestry University;Engineering Research Center of Forest Bio-preparation, Ministry of Education, Northeast Forestry University, Harbin 150040, China
  • Received:2007-12-30 Revised:1900-01-01 Online:2008-12-30 Published:2008-12-30

Abstract: A high-performance liquid chromatography(HPLC) method was established for determining contents of vitexin and isovitexin in the leaves, stems and roots of pigeonpea (Cajanus cajan(L.)Millsp). The analytes were separated on a HIQ Sil C18V(250 mm×4.6 mm,5 μm) column, using methanol-water-formic acid(35:64.74:0.26, volume ratio), as mobile phase, detection wavelength 330 nm, flow rate 1 mL/min, injection volume 10 μL, and column temperature 30℃. The calibration curves of vitexin and isovitexin showed good linearity within the ranges of 2-200 mg/L. For vitexin, relative standard deviation(RSD) of precision was not higher than 2.93%, RSD of repeatability was 2.91%, and recovery was 97.38%; for isovitexin, RSD of precision was not higher than 2.69%, RSD of repeatability was 3.37%, and recovery was 98.63%. Mass factions of vitexin and isovitexin in pigeonpea leaves, stems and roots were 0.768, 0.066, 0.183 mg/g and 0.799, 0.139, 0.013 mg/g, respectively. Pigeonpea leaves contain much higher amounts of vitexin and isovitexin than stems and roots, which could be developed as a good resource for obtaining vitexin and isovitexin on large scale.

Key words: pigeonpea, vitexin, isovitexin

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