Abstract: The effects of conditions of enzymolysis of cellulose from waste corrugated paper on glucose production, as well as the variation of crystallinity and microstructure of fibers during enzymolysis are investigated. The optimum conditions of temperature 50℃, pH value 4.8, enzyme loading 80FPU/g and substrate concentration 100g/L, after 36h of hydrolysis, 53.8g/L of glucose concentration and 76.9% of cellulose hydrolysis efficiency are obtained. The modification of crystallinity of fibers during hydrolysis is carried out in two stages. Hydrolysis mainly occurs at amorphous region firstly, causing the increase of crystallinity. Then the hydrolysis of both amorphous and crystallinic regions occurs, resulting in the crystallinity keeping stable. The photos of scanning electronic microscopy show that during hydrolysis the fragments are decorticated from the fibers, in succession the fibers are broken off and become hollow, and the structure of the fibers is completely destroyed.

Key words: waste corrugated paper, enzymolysis, glucose, crystallinity, microstructure