Abstract: The dreg-cakes of oil camellia(Camellia deifera Abel.)were extracted with 65% ethanol. The extract was separated by middle-pressure chromatography. The flavoniod glycosides mixture in high purity was obtained two compounds were isolated by HPLC, using XB-C18 column (Φ10mm×250mm,5μm) with a mobile phase which consists of a mixture of methanol and water (volume ratio 2:3). It had a flow rate of 3.0mL/min. UV detection was made at 230nm. According of IR, MS and NMR, the two compounds were identified to be kaempferol 3-O-[2-O-β-D--galactopyranosy-6-O-α-L-rhamnopyranosyl]-β-D-glucopyranoside(Ⅰ)and kaempferol 3-O-[2-O-β-D-xylocopyranosy-6-O-α-L-rhamnopyranosyl]-β-D-glucopyranoside(Ⅱ), respectively.

Key words: Camellia oleifera Abel. cake, flavonoid glycosides, HPLC, isolation