高速逆流色谱分离青叶胆中的生物碱

›› 2002, Vol. 22 ›› Issue (1): 74-76.

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SEPARATION OF ALKALOIDS FROM SWERTIA YUNNANENSIS BY HIGH-SPEED COUNTERCURRENT CHROMATOGRAPHY

ZI Min, YUAN Li-ming, LIU Pin, AI Ping, CHEN Ye-gao

School of Chemistry and Chemical Engineering, Yunnan Normal University, Kunming 650092, China

Received:2001-06-14 Revised:1900-01-01 Online:2002-03-30 Published:2002-03-30

Abstract

Abstract: Alkaloids from Swertia yunnanensis were isolated successfully by high speed countercurrent chromatography with the two phase solvent system CHCl 3:CH 3 OH:NaH 2 PO 4 (pH 4.8)3:2.5:1 to obtain 5 components,in which 3 components exhibited orange red color with bismuth potassium iodide reagent.Results were verified by TLC and five components were isolated pure.One was characterized as gentianine(30%)by spectroscopic analysis.

Key words: alkaloid, Swertia yunnanensis, high-speed countercurrent chromatography

CLC Number:

O657.7

ZI Min;YUAN Li-ming;LIU Pin;AI Ping;CHEN Ye-gao . SEPARATION OF ALKALOIDS FROM SWERTIA YUNNANENSIS BY HIGH-SPEED COUNTERCURRENT CHROMATOGRAPHY[J]. , 2002, 22(1): 74-76.

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SEPARATION OF ALKALOIDS FROM SWERTIA YUNNANENSIS BY HIGH-SPEED COUNTERCURRENT CHROMATOGRAPHY

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