Abstract: A method for content determination of imperatorin in Angelica dahurica(Fisch.) Bench.et Hook.by HPLC was established. The HPLC separation was performed on a hypersil ODS2 column(250mm×4.6mm, 5μm), at flow rate 1 mL/min, with mobile phase of methanol-water (65:35, volume ratio) and detected at wavelength 254nm. Using uniform design, the optimal conditions for imperatorin extraction were explored to be ultrasonically extracted by 78% ethanol at 20℃ for 22min. Imperatorin contents in A.dahurica seeds from different areas were determined. The highest imperatorin content was 1.722%, from Suiyang, then from Hulin and Hailin which were1.519%and 1.031%respectively, and the lowest was 0.698%, from Daxing'anling.

Key words: Angelica dahurica(Fisch.)Bench.et Hook., imperatorin, HPLC, uniform design