Abstract: Five types of macroporous resin were studied in order to compare their performances in absorbing and desorbing azadirachtin A. XAD-1180 was considered as the optimal macroporous resin for azadirachtin A. The dynamic adsorption and desorption conditions for purification of azadirachtin A were studied. The appropriate adsorption conditions were: concentration of azadirachtin A 2 mg/mL in 30% methanol solution, flow rate 1 BV/h, amount of saturated adsorption toward azadirachtin A 4-5 BV. The desorption conditions were: gradient elution with 50%,60% and 70% methanol solution at flow rates 1 and 0.5 BV/h. The purity of azadirachtin A purified by XAD-1180 for one time was 85.15%.Its purity was 93.1% when purified twice. The purified product was further confirmed to be azadirachtin A by HPLC-MS.

Key words: azadirachtin A, macroporous resin, dynamic absorbing