青梅黄酮类化合物的微乳薄层色谱分离鉴定

›› 2006, Vol. 26 ›› Issue (2): 53-56,6.

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A Study on Separation and Identification of Flavonoids in Plum by Microemulsion Thin-layer Chromatography

HAN Ming¹, ZENG Qing-xiao¹, XIAO Geng-sheng², CHEN Wei-dong², XU Yu-juan²

1. Department of Food and Technology, South China University of Technology, Guangzhou 510640, China;2. Key Laboratory of Fruit and Vegetable Processing of Guangdong, Guangzhou 510640, China

Received:2004-11-02 Revised:1900-01-01 Online:2006-06-30 Published:2006-06-30

Abstract

Abstract: A new method on separation and identification of flavonoids in plum was studied. Influence of microemulsion type on resolving power toward flavonoids was investigated with sodium dodecyl sulfate/n-C₄H₉OH/n-C₇H₁₆/H₂O microemulsion as eluant on polyamide film. The flavonoids were separated entirely. Six flavonoid spots were obtained from fruit peel and three from pulp, but they were not identical. Compared with the general mobile phase composed of C₄H₉OH/CH₃COOH/H₂O(4:1:5), the sensitivity of detection was improved markedly and the results were satisfactory. This new chromatographic method is simple, accurate for separation and identification of flavonoids in plum.

Key words: plum, flavonoids, microemulsion, thin-layer chromatography

CLC Number:

HAN Ming;ZENG Qing-xiao;XIAO Geng-sheng;CHEN Wei-dong;XU Yu-juan. A Study on Separation and Identification of Flavonoids in Plum by Microemulsion Thin-layer Chromatography[J]. , 2006, 26(2): 53-56,6.

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A Study on Separation and Identification of Flavonoids in Plum by Microemulsion Thin-layer Chromatography

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