不同生长时间对美国皂荚壳皂苷的影响比较研究

doi:10.3969/j.issn.0253-2417.2021.03.003

Abstract

Abstract:

Saponins were extracted from Gleditsia triacanthos L. shell by using anhydrous ethanol.The component analysis, saponin yield, sugar content and composition on saponins and surface activity of saponins in G. triacanthos shell at different growth time were systematically studied. The results showed that the saponin yield increased with the growth time increasing. The maximum mass fraction of saponin reached 14.21% at 25 weeks after flowering. The HPLC method indicated that the sugar chains of G. triacanthos saponins were mainly composed of glucose, xylose, arabinose and rhamnose. The content of hydrophilic sugar groups on saponin decreased first and then increased with the growth time increasing, and reached the lowest at 21 weeks.The surface activity of saponins was affected by the content of hydrophilic sugar groups. Meanwhile, the critical micelle concentration of saponins in G. triacanthos shell also decreased first and then increased with the growth time increasing. The critical micelle concentration was the lowest(0.07 g/L) at 21 weeks, and the surface tension was 44.57 mN/m. G. triacanthos shell saponins performed the best surface activity at 21 weeks, which were the most suitable for the production of surfactants. The results of FT-IR spectra confirmed that the extracted and purified saponins from G. triacanthos shell had the basic characteristic peaks of saponins.

Key words: Gleditsia triacanthos shell saponins, growth time, critical micelle concentration, sugar group, surface activity

CLC Number:

TQ35
TQ423

Wei XU, Zhiqiang XIAO, Chunyi LI, Jianxin JIANG, Liwei ZHU. Comparative Study on Properties of Gleditsia triacanthos L. Shell Saponins at Different Growth Time[J]. Chemistry and Industry of Forest Products, 2021, 41(3): 19-25.

Figures/Tables 7

Table 1

Fig.1

Fig.2

Table 2

Fig.3

Fig.4

Table 3

References 22

1	SCHNABEL A , LAUSHMAN R H , HAMRICK J L . Comparative genetic structure of two co-occurring tree species, Maclura pomifera (moraceae) and Gleditsia triacanthos(legu minosae)[J]. Heredity, 1991, 67, 357- 364. doi: 10.1038/hdy.1991.99
2	ZHANG Z Z , KAZUO K , JIA Z H , et al. Four new triterpenoidal saponins acylated with one monoterpenic acid from Gleditsia sinensis[J]. Journal of Natural Products, 1999, 62, 740- 745. doi: 10.1021/np980441k
3	杨丹霞. 茶皂苷油水界面组装行为及其稳定的风味乳液、油粉及油胶构建[D]. 广州: 华南理工大学, 2018.
	YANG D X. Fabrication of flavor emulsion, oil powder and olegel based on the self-assembly behavior of natural tea saponin at oil-water interface[D]. Guangzhou: South China University of Technology, 2018.
4	倪付花, 桑青, 陈敏, 等. 皂荚皂苷的提取及其抑菌作用的研究[J]. 时珍国医国药, 2012, 23 (2): 351- 352. doi: 10.3969/j.issn.1008-0805.2012.02.043
	NI F H , SANG Q , CHEN M , et al. Study on extraction of saponins from Gleditsia sinensis and its antibacterial activity[J]. Lishizhen Medicine and Materia Medica Research, 2012, 23 (2): 351- 352. doi: 10.3969/j.issn.1008-0805.2012.02.043
5	吕喆璇, 张威伟, 刘丹丹, 等. 肥皂荚皂苷提取工艺优化及其表面张力研究[J]. 林产化学与工业, 2019, 39 (6): 102- 108.
	LYU Z X , ZHANG W W , LIU D D , et al. Extraction technology and surface tension characterization of Gymnocladus chinensis saponin[J]. Chemistry and Industry of Forest Products, 2019, 39 (6): 102- 108.
6	廖晓霞, 朱莉伟, 彭茜, 等. 皂荚皂素和油茶皂素表面活性研究[J]. 林产化学与工业, 2009, 29 (2): 69- 74. doi: 10.3321/j.issn:0253-2417.2009.02.014
	LIAO X X , ZHU L W , PENG X , et al. Study on surface activity of Gleditsia saponin and Camellia saponin[J]. Chemistry and Industry of Forest Products, 2009, 29 (2): 69- 74. doi: 10.3321/j.issn:0253-2417.2009.02.014
7	KASSEM L A A , RAGAB M F , MARIAN N , et al. Two new acylated triterpenoidal saponins from Gleditsia caspica Desf.and the effect of its saponin content on LPS-induced cognitive impairment in mice[J]. Phytochemistry Letters, 2020, 40, 53- 62. doi: 10.1016/j.phytol.2020.09.007
8	SLUITER A, HAMES B, et al. Deter mination of structural carbohydrates and lignin in biomass: Laboratory analytical procedure(LAP): NREL/TP-510-42618[G]. Golden: National Renewable Energy Laboratory, 2012-08-03.
9	康家胜, 方春, 张静, 等. AB-8型树脂对无患子皂苷的动态吸附与解吸性能[J]. 天然产物研究与开发, 2011, 23 (4): 709- 712.
	KANG J S , FANG C , ZHANG J , et al. Dynamic adsorption and desorption of sapindus-saponins pnto AB-8 resin[J]. Natural Product Research and Development, 2011, 23 (4): 709- 712.
10	CHEN Y F , YANG C H , CHANG M S , et al. Foam properties and detergent abilities of the saponins from Camellia oleifera[J]. International Journal of Molecular Siences, 2020, 11 (11): 4417- 4425.
11	李莹桥. 山皂荚皂苷的提取工艺及其表面活性剂的性能分析[D]. 长春: 吉林农业大学, 2011.
	LI Y Q. Analysis the properties of surfactant and extraction technique of saponin from Gleditisia japonica Miq[D]. Changchun: Jilin Agricultural University, 2011.
12	SZAKIEL A , PACZKOWSKI C , HENRY M . Influence of environmental abiotic factors on the content of saponins in plants[J]. Phytochemistry Reviews, 2011, 10 (4): 471- 491. doi: 10.1007/s11101-010-9177-x
13	邵金良, 袁唯, 董文明, 等. 皂荚的功能成分及综合利用[J]. 中国食物与营养, 2005, 4, 23- 25.
	SHAO J L , YUAN W , DONG W M , et al. Functional components and comprehensive utilization of Gleditsia sinensis[J]. Food and Nutrition in China, 2005, 4, 23- 25.
14	VINCKEN J P , HENG L , GROOT A , et al. Saponins, classification and occurence in the plant kingdom[J]. Phytochemistry, 2007, 68, 275- 297. doi: 10.1016/j.phytochem.2006.10.008
15	ZHANG Z , KOIKE K , JIA Z , et al. Triterpenoidal saponins acylated with two monoterpenic acids from Gleditsia sinensis[J]. Chemical and Pharmaceutical Bulletin, 1999, 47 (3): 388- 393. doi: 10.1248/cpb.47.388
16	YU J , LI G , MU Y , et al. Anti-breast cancer triterpenoid saponins from the thorns of Gleditsia sinensis[J]. Natural Product Research, 2018, 33 (16): 1- 6.
17	BUCKERIDGE M S . Seed cell wall storage polysaccharides: Models to understand cell wall biosynthesis and degradation[J]. Plant Physiology, 2010, 154, 1017- 1023. doi: 10.1104/pp.110.158642
18	CAMPBELL J M , REID J S G . Galactomannan formation and guanosine 5'-diphosphate-mannose: Galactomannan mannosyltransferase in developing seeds of fenugreek(Trigonella foenum-graecum L., leguminosae)[J]. Planta, 1982, 155, 105- 111. doi: 10.1007/BF00392539
19	SHUKLA D , TYAGI V K . Cationic gemini surfactants: A review[J]. Journal of Oleo Science, 2006, 55, 381- 390. doi: 10.5650/jos.55.381
20	夏春华, 朱全芬, 田洁华, 等. 茶皂素的表面活性及其相关的功能性质[J]. 茶叶科学, 1990, 1, 1- 10.
	XIA C H , ZHU Q F , TIAN J H , et al. Surface activity and related functional properties of tea saponin[J]. Journal of Tea Science, 1990, 1, 1- 10.
21	XING Y , BU L , SUN D , et al. Enhancement of high-solids enzymatic hydrolysis and fermentation of furfural residues by addition of Gleditsia saponin[J]. Fuel, 2016, 177, 142- 147. doi: 10.1016/j.fuel.2016.03.024
22	YANG S J , YU H , YOU Y , et al. Effective lactic acid production from waste paper using Streptococcus thermophilus at low enzyme loading assisted by Gleditsia saponin[J]. Carbohydrate Polymers, 2018, 200, 122- 127. doi: 10.1016/j.carbpol.2018.07.063

采摘时间/周 sampling time/week	皂苷/% saponins	脂肪/% fat	灰分/% ash	木质素/% lignin	纤维素/% cellulose	半纤维素/% hemicellulose
15	11.17	1.04	2.90	12.94	24.57	11.93
17	11.85	1.30	3.55	15.02	24.99	11.56
19	11.92	1.39	3.19	14.34	24.69	12.06
21	12.05	1.13	3.83	16.05	23.81	9.80
23	13.82	1.02	3.15	14.32	24.02	11.22
25	14.21	1.05	2.95	12.22	23.35	11.19

采摘时间/周 sampling time/week	甘露聚糖/% mannan	半乳聚糖/% galactan	半乳甘露聚糖/% galactomannan
15	56.93±0.11	22.52±0.09	79.45±0.02
17	60.64±0.27	21.64±0.03	82.28±0.35
19	62.59±0.18	20.65±0.05	83.25±0.67
21	63.39±0.15	19.96±0.06	83.54±0.21
23	63.85±0.01	19.70±0.02	83.55±0.01
25	61.66±0.04	19.53±0.04	81.19±0.04

采摘时间/周 sampling time/week	临界胶束浓度/(g·L^-1) critical micelle concentration	表面张力/(mN·m^-1) surface tension
15	0.50	43.43
17	0.30	44.43
19	0.25	43.20
21	0.07	44.57
23	0.10	44.10
25	0.25	43.80

[1]	LI Man, PEI Lijun, CAI Zhaosheng, FANG Guigan. Synthesis,Characterization and Properties of Amphoteric Polymeric Surfactant CHPDMDHA-g-N,O-CMC [J]. Chemistry and Industry of Forest Products, 2018, 38(1): 115-120.
[2]	PEI Li-jun, CAI Zhao-sheng, SHANG Shi-bin, SONG Zhan-qian. Synthesis and Performance of Dehydroabietylamine-chitosan Cationic Surfactants [J]. Chemistry and Industry of Forest Products, 2014, 34(5): 47-52.
[3]	ZHU Xue-mei, JIANG Shi-meng, CAI Zhao-sheng, CHEN Zhi-dong. Preparation of 3-Chloro-2-hydroxypropyl Dimethyl Dehydroabietyl Ammonium Chloride and Its Surface Activities [J]. Chemistry and Industry of Forest Products, 2014, 34(3): 116-120.
[4]	DING Yu-qiu, LIU Shao-gang, LI Long, DING Yu-bo, DIAO Kai-sheng. The Synthesis of N,N-Dimethyl Dehydroabietylamine Oxide and Its Properties [J]. Chemistry and Industry of Forest Products, 2014, 34(1): 43-48.
[5]	HAN Shi-yan;LI Shu-jun;ZHAO Yan-yan;SONG Zhan-qian;FANG Gui-zhen. Effect of Spacer Chain Character on Surface Activities of Rosin-based Quaternary Ammonium Gemini Surfactants [J]. , 2012, 32(4): 39-42.
[6]	HAN Shi-yan;SONG Zhan-qian;FANG Gui-zhen;LI Shu-jun. Synthesis and Analysis of Rosin-based Quaternary Ammonium Gemini Surfactant [J]. , 2009, 29(S1): 110-112,.
[7]	ZHOU Yong-hong;SONG Zhan-qian;XU Yu-zhi;XIE Hui. STUDY ON SURFACE ACTIVITY OF ROSIN PENTAERYTHRITOL ESTER POLYOXYLETHYLENE ETHER [J]. , 2004, 24(S1): 61-64.
[8]	TU Bin;LU Zhuo-min;CHEN Fan-geng. ELECTROCHEMICAL DEGRADATION OF SODIUM LIGNOSULFONATE [J]. , 2002, 22(2): 21-25.
[9]	CHEN Fan-geng;LU Zhuo-min;TU Bin . ALKYLATION OF WHEAT STRAW ALKALINE LIGNIN [J]. , 2001, 21(2): 39-43.
[10]	REN Cheng-xia;LI Zhong-zheng. OXIDATIVE DEGRADATION CHARACTERISTICS OF THE PRODUCTS OF WHEAT STRAW ALKALINE LIGNIN [J]. , 2001, 21(04): 49-54.

Comparative Study on Properties of Gleditsia triacanthos L. Shell Saponins at Different Growth Time

RichHTML

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

Figures/Tables 7

References 22

Related Articles 10

Recommended Articles

Metrics

Comments