碱性离子液体TBAH预处理对桉木结构和酶解性能的影响

doi:10.3969/j.issn.0253-2417.2022.01.008

Abstract

Abstract:

In order to explore the mechanism of the alkaline ionic liquids tetrabutylammonium hydroxide (TBAH) in the pretreatment of eucalyptus, the response surface analysis method was used to design the model, and the optimal conditions[JP]for the pretreatment of eucalyptus with ionic liquid TBAH were pretreatment time 57.19 min, pretreatment[JP]temperature 71.98℃, and TBAH mass fraction 11.78%.The model was proved to be scientific accurate and practical.By comparing the composition[JP]of feedstock, NaOH, tetrabutylammonium fluoride (TBAF) and TBAH pretreated eucalyptus samples, Fourier infrared spectroscopy (FT-IR), X-ray diffraction (XRD) and scanning electron microscopy (SEM) were carried out for comparing the composition of feedstock with that of NaOH, tetrabutylammonium fluaride (TBAF) and TBAH pretreating eucalyptus samples, which aimed to analyze pretreatment reaction mechanism.The results showed that in the pretreatment, [TBA]⁺ could effectively degrade lignin and reduce the crystallinity; OH^- could degrade hemicellulose.Under the combined action of[TBA]⁺ and OH^-, the hemicellulose of eucalyptus samples was degraded from 26.8% to 18.0%, and the lignin was degraded from 26.3% to 15.0%;the crystallization index increased[JP]from 42.39% to 54.11%;and TBAH pretreatment would destroy the complete, dense and uniform structure of the surface of eucalyptus samples; TBAH pretreated eucalyptus wood sample was enzymatically hydrolyzed for 72 h, the yield of reducing sugar was 365.62 mg/g, and the conversion rate of cellulose was 69.24%.

Key words: alkaline ionic liquid, pretreatment, enzymatic hydrolysis efficiency, response surface analysis

CLC Number:

TQ35

Shujie WANG, Xianfeng HOU, Yingshi HUANG, Xiaofeng CHEN, Jin SUN, Zhenzhong GAO. Effect of Alkaline Ionic Liquid TBAH Pretreatment on Structure and Enzymatic Properties of Eucalyptus[J]. Chemistry and Industry of Forest Products, 2022, 42(1): 57-63.

Figures/Tables 7

Table 1

Table 2

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Table 3

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References 23

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方差来源 variance source	平方和 sum of squares	自由度 d_f	均方值 mean square	F值 F value	P值 P value	显著性 significant
模型model	30647.29	9	3405.254	14.68	0.00023	**
X₁	236.6263	1	236.6263	1.02	0.339075
X₂	839.5086	1	839.5086	3.62	0.089661
X₃	8166.023	1	8166.023	35.17	0.000221	**
X₁X₂	313.1253	1	313.1253	1.35	0.275376
X₁X₃	215.6998	1	215.6998	0.93	0.360284
X₂X₃	0.445598	1	0.445598	0.002	0.966012
X₁²	5121.787	1	5121.787	22.06	0.001125	**
X₂²	16643.41	1	16643.41	71.69	0.000014	**
X₃²	8157.244	1	8157.244	35.14	0.000221	**
残差residual	2089.481	9	232.1645
失拟项lack of fit	1607.604	5	321.5209	2.67	0.181422
纯误差pure error	481.8766	4	120.4691
总和cor total	32736.77	18

样品 sample	纤维素/% cellulose	半纤维素/% hemicellulose	木质素/% lignin	灰分/% ash	还原糖产率/(mg·g^-1) sugar yield
未处理feedstock	42.4	26.8	26.3	0.1	125.73
TBAF预处理TBAF pretreatment	50.5	22.9	15.9	0.1	301.43
NaOH预处理NaOH pretreatment	50.2	16.3	22.3	0.5	356.91
TBAH预处理TBAH pretreatment	52.8	18.0	15.0	0.6	365.62

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Effect of Alkaline Ionic Liquid TBAH Pretreatment on Structure and Enzymatic Properties of Eucalyptus

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序号 No.	X₁ TBAH质量分数/% TBAH mass fraction	X₂ 时间/min time	X₃ 温度/℃ temperature	还原糖产率/ (mg·g^-1) sugar yield
1	12	60	60	359.318
2	20	60	60	294.866
3	16	40	80	314.027
4	12	60	100	340.56
5	12	100	60	254.41
6	12	60	20	236.18
7	12	20	60	258.721
8	16	80	40	251.16
9	16	40	40	281.95
10	8	80	80	283.761
11	4	60	60	312.7
12	8	80	40	271.51
13	8	40	40	295.302
14	8	40	80	338.632
15	16	80	80	316.205
16	12	60	60	368.329
17	12	60	60	350.43
18	12	60	60	348.29
19	12	60	60	373.54