檀香叶中的γ-氨基丁酸的HPLC测定研究

doi:10.3969/j.issn.0253-2417.2013.02.024

›› 2013, Vol. 33 ›› Issue (2): 134-138.doi: 10.3969/j.issn.0253-2417.2013.02.024

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Determination of γ-Aminobutyric Acid in Leaves of Santalum album L. by HPLC

YANG Yan¹, HE Li-ping^1,2, GAO Xiang-yang¹

1. College of Food Science, South China Agricultural University, Guangzhou 510642, China;2. Instrumental Analysis & Research Center, South China Agricultural University, Guangzhou 510642, China

Received:2012-03-19 Revised:1900-01-01 Online:2013-04-30 Published:2013-04-30

Abstract

Abstract: A method was established by reversed-phase high performance liquid chromatography for determining the content of γ-aminobutyric acid (GABA) in leaves of Santalum album L. The sample was pretreated by soxhlet extraction to eliminate the pigments with ether, and then by water extraction and alcohol precipitation to eliminate sugar and protein. GABA was derivatized with dansyl chloride (Dns-Cl) and isolated by reversed-phase HPLC. GABA was detected quantitatively by HPLC with fluorescence detection. Nice linear relationship between peak area and concentration was obtained with the linear regression equation as y=475 211x+69 037,R²=0.999 8. The recovery of standard addition was in the range of 112% to 120%. The detection limit of GABA was 7 μg/g. The established HPLC method is simple, economic, stable and accurate with good reproducibility. It can be applied on determination of GABA in leaves of Santalum album L.

Key words: leaves of Santalum album L., γ-aminobutyric acid(GABA), dansyl chloride, HPLC

CLC Number:

TQ351.0

YANG Yan;HE Li-ping;GAO Xiang-yang. Determination of γ-Aminobutyric Acid in Leaves of Santalum album L. by HPLC[J]. , 2013, 33(2): 134-138.

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Determination of γ-Aminobutyric Acid in Leaves of Santalum album L. by HPLC

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