桉木APMP制浆废水抽出物提取及其抑藻效应研究

doi:10.3969/j.issn.0253-2417.2017.02.007

摘要/Abstract

摘要： 采用乙酸乙酯和甲基叔丁基醚2种有机溶剂对自制桉木碱性过氧化氢机械浆（APMP）制浆废水进行有机物提取，采用GC-MS分析对比了2种溶剂抽出物的组成。结果表明：乙酸乙酯提取的抽提物得率为204 mg/L，甲基叔丁基醚提取得率为132 mg/L，经GC-MS分析表明2种溶剂提取的物质种类分别为44种和49种，主要包含有机酸类、醇类、酯类和酚类物质，其中酸类物质的含量最高，分别为76%和80%左右。将乙酸乙酯抽提物应用于铜绿微囊藻（Microcystis aeruginosa）的抑藻效应研究，发现不同浓度的化感物质对铜绿微囊藻均具有一定程度的抑制作用，且随浓度增加抑藻效应增强。较低浓度的化感物质对铜绿微囊藻有抑制作用但不显著；高浓度的化感物质对铜绿微囊藻具有显著抑制效应，50、100 mg/L时培养7天抑制率分别达到24.8%和77.2%；藻液在446 nm的吸光值测定结果进一步证明了APMP制浆废水中提取的有机物质具有抑藻效果。

关键词: 化感物质, 抑制作用, APMP制浆废水, 铜绿微囊藻

Abstract: Eucalyptus APMP effluents were extractedby ethyl acetate (EA) and methyl tertiary butyl ether (MTBE) and the extractives were anaylyzes by GC-MS. The results showed that the yield of EA extractive was 204 mg/L with 44 kinds of organic compounds, and the yield of MTBE extractive was 132 mg/L with 49 kinds of organic compounds. The extractives were composed by acids, alcohols and esters, in which acids were the main compounds accounting for about 76% (extracted by EA) and 80% (extracted by MTBE), respectively. Inhibition effect of EA extractives on the growth of Microcystis aeruginosa was investigated. The results showed that the extractives from APMP effluents inhibited the growth of M. aeruginosa with different effect in different concentrations. The inhibition effect was not remarkable at low extractive concentrations, and the inhibition effect was significant when the concentrations were over 50 and 100 mg/L, with the inhibition rates of 24.8% and 77.2%, respectively. The inhibition effect would be further investigated through the absorbent under 446 nm.

Key words: allelochemicals, inhibition, APMP effluents, Microcystis aeruginosa

中图分类号:

程富江, 刘廷志, 李祥祥, 亓晖, 钟丽鸣. 桉木APMP制浆废水抽出物提取及其抑藻效应研究[J]. 林产化学与工业, 2017, 37(2): 57-64.

CHENG Fujiang, LIU Tingzhi, LI Xiangxiang, QI Hui, ZHONG Liming. Extractives of Organics from Eucalyptus APMP Effluents and Its Inhibition Effect on Growth of Microcystis aeruginosa[J]. Chemistry and Industry of Forest Products, 2017, 37(2): 57-64.

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