核桃楸皮提取物对人肝癌细胞SMMC-7721的增殖抑制作用及对细胞周期的影响

doi:10.3969/j.issn.0253-2417.2017.03.019

林产化学与工业 ›› 2017, Vol. 37 ›› Issue (3): 136-140.doi: 10.3969/j.issn.0253-2417.2017.03.019

核桃楸皮提取物对人肝癌细胞SMMC-7721的增殖抑制作用及对细胞周期的影响

梁启超¹, 刘爽², 张朝立¹

1. 牡丹江医学院, 黑龙江牡丹江 157011;
2. 牡丹江心血管病医院, 黑龙江牡丹江 157011

收稿日期:2016-09-25 出版日期:2017-06-25 发布日期:2017-07-03
通讯作者: 张朝立(1979-),男,讲师,博士,研究领域为天然产物化学研究;E-mail:chaolizhang79@163.com E-mail:chaolizhang79@163.com
作者简介:梁启超(1985-),男,黑龙江佳木斯人,硕士,实验师,主要从事天然产物化学研究;E-mail:xiaochao9199@163.com
基金资助:
黑龙江省自然科学基金项目（H201692）；黑龙江省教育厅科学技术研究项目（12531742）；牡丹江医学院科学技术研究项目（ZS201515）

Effect of Extracts from Bark of Juglans mandshurica on Proliferation and Cell Cycle of SMMC-7721 Cells

LIANG Qichao¹, LIU Shuang², ZHANG Zhaoli¹

1. Mudanjiang Medical College, Mudanjiang 157011, China;
2. Mudanjiang Cardiovascular Hospital, Mudanjiang 157011, China

Received:2016-09-25 Online:2017-06-25 Published:2017-07-03

摘要/Abstract

摘要： 研究核桃楸皮提取物（EMBJM）对人肝癌细胞SMMC-7721的增殖抑制作用及对细胞周期的影响。体外培养SMMC-7721细胞，并用不同浓度核桃楸皮甲醇提取物处理。通过MTT法研究EMBJM对SMMC-7721细胞增殖活性的影响；采用流式细胞仪分析EMBJM对SMMC-7721细胞周期的影响。EMBJM可以显著抑制SMMC-7721细胞生长，具有时间和计量依赖性。细胞周期实验表明EMBJM将SMMC-7721细胞阻滞在G2/M和S期，并具有剂量依赖性。EMBJM质量浓度为8和16mg/L时， G2/M期细胞所占百分比分别为13.89%±2.46%和14.35%±2.68%，与空白对照组（8.34%±1.42%）比较，差异均有统计学意义（P<0.05）；同等浓度范围内S期细胞所占百分比分别为39.87%±2.83%和44.81%±2.93%，与空白对照组（26.80%±1.76%）比较，差异均有统计学意义（P<0.05）。桃楸皮甲醇提取物可以影响SMMC-7721细胞周期分布和有效抑制SMMC-7721细胞生长。

关键词: 核桃楸皮, 细胞毒性, 细胞周期, SMMC-7721细胞

Abstract: To study effect of extracts from bark of Juglans mandshurica (EMBJM) on proliferation and cell cycle of SMMC-7721 cells, the SMMC-7721 cells in culture medium in vitro were given different concentrations of EMBJM. The inhibition rate of EMBJM on the SMMC-7721 cell was detected by MTT assay. The cell cycle analyzed was detected by flow cytometry (FCM). The EMBJM exhibited a significant inhibition on SMMC-7721 cells time-and dose-dependently. The cell cycle detection results suggested that EMBJM could arrest SMMC-7721 cells specifically in growth 2/mitosis(G2/M) and synthesis(S) phase cell cycle in a dose-dependent manner. Compared with control group (8.34%±1.42%; 26.80%±1.76%), the G2/M and S phase of SMMC-7721 cells exposed to EMBJM (13.89%±2.46%, 39.87%±2.83% (8 mg/L);(14.35%±2.68%, 44.81%±2.93% (16 mg/L)) were significantly increased (P<0.05, n=3). EMBJM can effectively inhibited proliferation in SMMC-7721 cells and effected cell cycle distribution.

Key words: bark of Juglans mandshurica, cytotoxicity, cell cycle, SMMC-7721 cells

中图分类号:

TQ35

梁启超, 刘爽, 张朝立. 核桃楸皮提取物对人肝癌细胞SMMC-7721的增殖抑制作用及对细胞周期的影响[J]. 林产化学与工业, 2017, 37(3): 136-140.

LIANG Qichao, LIU Shuang, ZHANG Zhaoli. Effect of Extracts from Bark of Juglans mandshurica on Proliferation and Cell Cycle of SMMC-7721 Cells[J]. Chemistry and Industry of Forest Products, 2017, 37(3): 136-140.

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核桃楸皮提取物对人肝癌细胞SMMC-7721的增殖抑制作用及对细胞周期的影响

Effect of Extracts from Bark of Juglans mandshurica on Proliferation and Cell Cycle of SMMC-7721 Cells

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