预处理对蔗渣吸附纤维素酶特性的影响

doi:10.3969/j.issn.0253-2417.2018.02.010

林产化学与工业 ›› 2018, Vol. 38 ›› Issue (2): 75-79.doi: 10.3969/j.issn.0253-2417.2018.02.010

预处理对蔗渣吸附纤维素酶特性的影响

董益帆, 刘志佳, 兰天晴

昆明理工大学食品安全研究院, 云南昆明 650500

收稿日期:2017-09-01 出版日期:2018-04-25 发布日期:2018-04-28
通讯作者: 兰天晴,讲师,博士,硕士生导师,研究领域为食品副产物高值化利用;E-mail:lantianqing_8019@163.com。 E-mail:lantianqing_8019@163.com
作者简介:董益帆(1993-),女,云南昆明人,硕士生,主要从事食品副产物高值化利用研究工作
基金资助:
国家自然科学基金资助项目（21766014）；云南省科技计划面上项目（2014FB119）；云南省人才培养计划项目（KKSY201405054）

Effect of Pretreatment Methods on Cellulase Adsorption Profiles of Sugarcane Bagasse

DONG Yifan, LIU Zhijia, LAN Tianqing

Food Safety Institute, Kunming University of Science and Technology, Kunming 650500, China

Received:2017-09-01 Online:2018-04-25 Published:2018-04-28

摘要/Abstract

摘要： 以亚硫酸盐（SPORL）和稀硫酸（DA）预处理蔗渣为底物，探讨两种预处理方法对蔗渣酶解的影响，并对酶解过程中蔗渣对纤维素酶的吸附特性进行探究，比较了两种底物的酶水解效率、成分、表面形貌、吸附等温线和吸附动力学等特征，结果显示：SPORL法预处理蔗渣的还原糖转化率（81.92%）明显高于DA法预处理蔗渣（60.89%）。与未处理蔗渣相比，DA与SPORL法预处理蔗渣表面变得粗糙，表面纤维被部分剥离，暴露出内部的纤维骨架。两种方法预处理蔗渣的纤维素和半纤维素含量也没有明显差异，但SPORL法预处理蔗渣中Klason木质素含量更低。X射线光电子能谱（XPS）图分析表明两种预处理蔗渣都对纤维素酶有明显吸附。吸附实验结果表明，SPORL法预处理蔗渣对纤维素酶最大吸附量为61.91 mg/g，低于DA法的82.69 mg/g，但SPORL法预处理蔗渣对纤维素酶的吸附亲和力及吸附强度均大于DA法。

关键词: 蔗渣, 亚硫酸盐预处理, 稀酸预处理, 纤维素酶水解, 纤维素酶吸附

Abstract: The effects of two pretreatment methods, sulfite (SPORL) pretreatment and dilute sulfuric acid (DA) pretreatment, on the enzymatic hydrolysis of sugarcane bagasse (SCB) substrates were investigated and the adsorption of cellulose on SCB during the hydrolysis was studied. The enzymatic hydrolysis efficiency, composition, surface morphology, adsorption isotherms and kinetics of two kinds of pretreated SCB were compared. The results showed that the enzymatic hydrolysis efficiency of SPORL-SCB(81.92%) was significantly higher than that of DA treated SCB(60.89%). Compared with the untreated SCB, the surface of SCB treated by SPORL and DA became rough, the surface fibres were partially stripped and the internal fibre skeleton was exposed. The holocellulose contents of these two treated SCB substrates were not significantly different, but the Klason lignin content of SPORL treated SCB was lower. The XPS images suggested that the treated SCB substrates both adsorbed cellulase. The adsorption isotherm and kinetics curves showed that the maximum adsorption of cellulase on SPORL treated SCB was 61.91 mg/g and lower than that on DA treated SCB(82.69 mg/g), but the adsorption affinity and adsorption strength of SPORL treated SCB were higher than those of the DA treated SCB.

Key words: sugarcane bagasse, sulfite pretreatment, dilute acid pretreatment, enzymatic hydrolysis, cellulase adsorption

中图分类号:

TQ353

董益帆, 刘志佳, 兰天晴. 预处理对蔗渣吸附纤维素酶特性的影响[J]. 林产化学与工业, 2018, 38(2): 75-79.

DONG Yifan, LIU Zhijia, LAN Tianqing. Effect of Pretreatment Methods on Cellulase Adsorption Profiles of Sugarcane Bagasse[J]. Chemistry and Industry of Forest Products, 2018, 38(2): 75-79.

参考文献

[1] 王钱钱,詹怀宇,孙建中,等.纤维素酶在木质纤维素底物上的吸附、脱附以及循环利用[J].造纸科学与技术,2014,33(3):9-14. WANG Q Q,ZHAN H Y,SUN J Z,et al.Cellulase adsorption-desorption behavior onto/from lignocellulosic substrates and potential recycling[J].Paper Science & Technology,2014,33(3):9-14.
[2] COSGROVE D J. Growth of the plant cell wall[J]. Nature Reviews Molecular Cell Biology,2005,6(11):850-861.
[3] 朱文远. SPORL预处理技术在木质生物质转化中应用研究[D].广州:华南理工大学博士学位论文,2010. ZHU W Y.On application of SPORL pretreatment process in woody biomass conversion[D].Guangzhou:Doctoral Dissertation of South China University of Technology,2010.
[4] 田顺风, 程力, 顾正彪, 等. 玉米秸秆中不同木质素脱除方法对纤维素酶吸附及酶解效果的比较[J].化工学报,2016,67(5):2085-2092. TIAN S F,CHENG L,GU Z B,et al.Comparison of different lignin removal processes for corn stover on cellulase adsorption and enzymatic hydrolysis[J].CIESC Journal,2016,67(5):2085-2092.
[5] SMITH P K, KROHN R I, HERMANSON G T, et al. Measurement of protein using bicinchoninic acid[J]. Analytical Biochemistry, 1985,150(1):76-85.
[6] DU R U, SU R X, LI X, et al.Controlled adsorption of cellulase onto pretreated corncob by pH adjustment[J].Cellulose, 2012, 19(2):371-380.
[7] AMIE D S, BONNIE H, ROGELIO R, et al. Determination of structural carbohydrates and lignin in biomass[S]. United States:National Renewable Laboratory Golden, 2005.
[8] 刘志佳, 董益帆, 兰天晴. 亚硫酸盐预处理对蔗渣酶解效率的影响[J].林产化学与工业, 2016, 36(5):89-94. LIU Z J, DONG Y F, LAN T Q.Effect of sulfite pretreatment on hydrolysis efficiency of sugarcane bagasse[J].Chemistry and Industry of Forest Products, 2016, 36(5):89-94.
[9] 刘志佳. 稀酸与亚硫酸盐预处理对蔗渣酶水解效率的影响研究[D].昆明:昆明理工大学硕士学位论文, 2016. LIU Z J.Study of the effect of dilute acid and sulfite pretreatment on hydrolysis efficiency of bagasse[D].Kunming:Master Degree Thesis of Kunming University of Science and Technology, 2016.
[10] LIU Z J, LAN T Q, LI H, et al. Effect of bisulfite treatment on composition,structure,enzymatic hydrolysis and cellulase adsorption profiles of sugarcane bagasse[J].Bioresource Technology, 2017, 223:27-33.
[11] YU Z Y, JAMEEL H, CHANG H M, et al.The effect of delignification of forest biomass on enzymatic hydroxide and oxidants[J].Bioresource Technology, 2011, 102(19):9083-9089.
[12] 张景强,李清春,林鹿.解结晶对纤维素吸附纤维素酶的影响[J].林产化学与工业,2016,36(1):105-111. ZHANG J Q, LI Q C, LIN L.Effect of decrystallization of cellulose on adsorbing cellulase[J].Chemistry and Industry of Forest Products,2016,36(1):105-111.
[13] GAN Q, ALLEN S J, TAYLOR G.Kinetic dynamics in heterogeneous enzymatic hydrolysis of cellulose:An overview, an experimental study and mathematical modelling[J].Process Biochemistry,2003,38(7):1003-1018.
[14] XU F, DING H S, OSBORN D, et al.Partition of enzymes between the solvent and insoluble substrate during the hydrolysis of lignocellulose by cellulases[J].Journal of Molecular Catalysis B:Enzymatic,2008,5l(1):42-48.

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预处理对蔗渣吸附纤维素酶特性的影响

Effect of Pretreatment Methods on Cellulase Adsorption Profiles of Sugarcane Bagasse

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