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林产化学与工业

林产化学与工业 ›› 2002, Vol. 22 ›› Issue (04): 83-86.

• 研究简报 • 上一篇    

耐热型木聚糖酶制备工艺的研究

鄢永亮, 夏黎明   

  1. 浙江大学, 化学工程与生物工程系, 浙江, 杭州, 310027
  • 收稿日期:2002-04-01 修回日期:1900-01-01 出版日期:2002-12-30 发布日期:2002-12-30
  • 通讯作者: 夏黎明

STUDY ON PREPARATION TECHNOLOGY OF THERMOSTABLE XYLANASE

YAN Yong-liang, XIA Li-ming   

  1. Department of Chemical Engineering and Bioengineering, Zhejiang University, Hangzhou 310027, China
  • Received:2002-04-01 Revised:1900-01-01 Online:2002-12-30 Published:2002-12-30

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摘要： 以木糖渣为碳源,利用耐热子囊菌(Thermoascus aurantiacus)液体发酵生产木聚糖酶,确立了最佳工艺参数为:木糖渣质量浓度4%、培养温度45℃、转速180r/min、培养基初始pH值4.8、初始C/N为7.1,木聚糖酶活力可达900U/mL。酶学性质研究表明,该酶反应的最适温度为75℃,最适pH值为4.8。该酶的耐热性强,在75℃下保温lh,酶活力剩余85%以上。

关键词: 耐热型木聚糖酶, 木糖渣, 液体发酵

Abstract: Preparation of xylanase by Thermoascus aurantiacus on corn cob residue was studied.Optimal fermentation parameters were:corn cob residue 4%;rotating speed 180 r/min;initial pH value of medium 4.8%;culture temperature 45℃;C/N of medium 7.1.Xylanase activity in the broth reached 900 U/mL.Optimal temperature and pH value for the xylanase reaction were 75℃ and 4.8,respectively.The enzyme preparation was thermo stable and had a good application potential.

Key words: Thermoascus aurantiacus, xylanase, corn cob residue, liquid fermentation

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