欢迎访问《林产化学与工业》，
林产化学与工业

林产化学与工业 ›› 2007, Vol. 27 ›› Issue (3): 33-36.

• 研究报告 • 上一篇    下一篇

基因重组酵母发酵木糖产酒精的研究

王周芳, 陈明, 王冰冰, 夏黎明   

  1. 浙江大学 化学工程与生物工程系, 浙江 杭州 310027
  • 收稿日期:2006-05-22 修回日期:1900-01-01 出版日期:2007-06-30 发布日期:2007-06-30
  • 通讯作者: 夏黎明,博士生导师,主要从事可再生资源利用方面的研究

Ethanol Fermentation on Xylose by a Recombinant Yeast

WANG Zhou-fang, CHEN Ming, WANG Bing-bing, XIA Li-ming   

  1. Department of Chemical Engineering and Bioengineering, Zhejiang University, Hangzhou 310027, China
  • Received:2006-05-22 Revised:1900-01-01 Online:2007-06-30 Published:2007-06-30

PDF (PC)

836

摘要： 以一株基因重组酵母为研究对象,探讨了厌氧条件下pH值、底物浓度、接种量、乙酸质量分数和葡萄糖添加量等关键因子对木糖发酵的影响.结果表明,重组酵母Saccharomyces cerevisiae ZU-10有较强的发酵木糖产乙醇的能力,在80g/L(相对木糖)、初始pH值5.5、接种量1.2g/L(细胞干质量,相对培养液)、30℃下发酵72h,发酵液中乙醇质量浓度达到28.9g/L.发酵液中的乙酸质量分数低于0.05%时对发酵木糖影响不大.添加适量的葡萄糖可促进木糖发酵,在30g/L木糖培养液中添加0g/L葡萄糖,36h内木糖利用率从79.0%增加到85.7%.

关键词: 木糖, 乙醇发酵, 基因重组酵母

Abstract: The key affecting factors of enthanol fermentation on xylose by a recombinant yeast of Saccharomyces cerevisiae ZU-10 were investigated. Fermentation was performed with 80 g/L xylose and inoculum of 1.2 g /L(cell dry weight) at initial pH value 5.5, 30℃ under anaerobic condition, and 28.9 g/L ethanol was obtained within 72 h. Acetic acid of mass fraction lower than 0.05% in culture medium caused minor influence on xylose fermentation. Addition of glucose to culture medium favored fermentation of xylose, and 85.7% xylose was utilized within 36 h at 40 g/L glucose in culture medium containing 30 g/L xylose.

Key words: xylose, fermentation, recombinant yeast

中图分类号: 

版权所有 © 中国林业科学研究院林产化学工业研究所　苏ICP备08107184号-3