皂荚多糖胶α-半乳糖苷酶修饰及产物性能研究

林产化学与工业 ›› 2007, Vol. 27 ›› Issue (5): 44-48.

皂荚多糖胶α-半乳糖苷酶修饰及产物性能研究

蒋建新¹, 朱莉伟¹, 王磊¹, 张卫明²

1. 北京林业大学材料科学与技术学院, 北京 100083;2. 南京野生植物研究院, 江苏南京 210042

收稿日期:2006-12-29 修回日期:1900-01-01 出版日期:2007-10-30 发布日期:2007-10-30

Studies on Modification of Polysaccharide Gum from Gleditsia sinensis Lam. Seeds with α-Galactosidase

JIANG Jian-xin¹, ZHU Li-wei¹, WANG Lei¹, ZHANG Wei-ming²

1. College of Materail Science and Technology, Beijing Forestry University, Beijing 100083, China;2. Nanjing Wild Plant Research Institute, Nanjing 210042, China

Received:2006-12-29 Revised:1900-01-01 Online:2007-10-30 Published:2007-10-30

摘要/Abstract

摘要： 皂荚多糖胶(GPG)和刺槐豆胶都属于半乳甘露聚糖型多糖胶.当多糖水溶液质量分数1%,皂荚多糖胶(半乳糖质量分数28.6%)与黄原胶的共混质量比为7:3,其凝胶强度为103g/cm2.刺槐豆胶(半乳糖质量分数20%)与黄原胶的共混凝胶强度最大,也是价格最高的半乳甘露聚糖胶.重点研究了α-半乳糖苷酶修饰皂荚多糖胶(EMGPG)得到与刺槐豆胶相似结构的半乳甘露聚糖,酶转化反应的最适宜底物质量分数从37℃的3%提高到50℃时的35%.20.2%半乳糖含量的酶法改性皂荚多糖胶与黄原胶共混后的凝胶强度为199 g/cm²,大于刺槐豆胶与黄原胶的共混凝胶强度(193g/cm²).X射线衍射分析表明,酶法改性皂荚多糖胶结晶度下降.

关键词: 皂荚, 多糖胶, α-半乳糖苷酶, 凝胶化

Abstract: Gleditsia sinensis Lam. polysaccharides gum (GPG) and locust bean gum were galactomannan gum. Mixed gel at 1% of total polysaccharide concentration showed the gel strength maximum of 103 g/cm² when the ratio of GPG (28.6% galactose) to xanthan was 7:3. Locust bean gum (20% galactose) is more functional and more expensive than other galactomannan gums. Investigation on removal of galactose residues from GPG by α-galactosidase to yield galactomannan similar to locust bean gum was carried out. The optimum mass fraction of substrate concentrations was increased from 3% at 37℃ to 35% at 50℃ due to increased enzymic temperature tolerance. Enzymically modified G. sinensis polysaccharides gum (EMGPG) with 20.2% galactose content showed maximum mixed gel strength of 199 g/cm², which was higher than that of locust bean gum (193 g/cm²). The crystallinity index for EMGPG is 0.235, which is somewhat lower than that of GPG (0.297).

Key words: Gleditsia sinensis Lam., polysaccharide gum, α-galactosidase, gelation

中图分类号:

TQ432.9
S713

蒋建新;朱莉伟;王磊;张卫明. 皂荚多糖胶α-半乳糖苷酶修饰及产物性能研究[J]. 林产化学与工业, 2007, 27(5): 44-48.

JIANG Jian-xin;ZHU Li-wei;WANG Lei;ZHANG Wei-ming. Studies on Modification of Polysaccharide Gum from Gleditsia sinensis Lam. Seeds with α-Galactosidase[J]. , 2007, 27(5): 44-48.

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