地榆根提取低聚原花青素的研究

林产化学与工业 ›› 2007, Vol. 27 ›› Issue (S1): 129-132.

地榆根提取低聚原花青素的研究

姜贵全¹, 方桂珍², 庞久寅¹

1. 北华大学林学院, 吉林吉林 132013;2. 东北林业大学材料科学与工程学院, 黑龙江哈尔滨 150040

收稿日期:2007-06-20 修回日期:1900-01-01 出版日期:2007-10-20 发布日期:2007-10-20
通讯作者: 方桂珍,教授,博士生导师,主要从事木材化学和木材功能性改良方面的研究。

Study on Extraction of Oligoprocyanidins from the Root of Sanguisorba officinalis Linn.

JIANG Gui-quan¹, FANG Gui-zhen², PANG Jiu-yin¹

1. Forestry College, Beihua University, Jilin 132013, China;2. College of Material Science and Engineering, Northeast Forestry University, Harbin 150040, China

Received:2007-06-20 Revised:1900-01-01 Online:2007-10-20 Published:2007-10-20

摘要/Abstract

摘要： 对吉林地区野生地榆根低聚原花青素(OPC)进行了提取分离工艺研究.考察了溶剂体积分数、原料粒度、料液比、浸提温度、浸提时间、浸提次数等6个因素对地榆根提取低聚原花青素得率的影响,确立了较佳提取条件为:以体积分数60%乙醇水溶液为提取剂、原料粒度为O.25～0.42 mm、料液比1:12(g:mL)、浸提温度50℃、浸提4次、每次1 h.浸提浓缩液加入一定量饱和NaCl溶液,低温离心过滤后,用2倍体积乙酸乙酯萃取3次,得到纯化后提取物中OPC质量分数达到56.86%,OPC得率为绝干原料的3.42%.

关键词: 地榆, 低聚原花青素, 提取工艺

Abstract: The extraction and separation techniques of oligoprocyanidins (OPC) from the root of wild Sanguisorba officinalis Linn. in Jilin were studied. The effects of solvent concentration, particle size, ratio of sample to solvent and extraction temperature, extraction time and extraction times on the yield of oligoprocyanidins were studied. The optimal extraction conditions are as follows: extraction solvent of 60% (volume fraction) alcohol solution, particle size 0.25-0.42 mm, ratio of sample to solvent 1:12(g:mL), extraction temperature 50℃, extraction times 4, 1 h once. The concentrated extraction liquid was added with definite amount of concentrated NaCl solution, then centrifuged under low temperature. The solution was extracted with double amounts of ethyl acetate for three times, to obtain the purified extract, in which the amount of oligoprocyanidins was 56.86%. The yield of oligoprocyanidins based on the bone-dried raw material is 3.42%.

Key words: Sanguisorba officinalis Linn., oligoprocyanidins, extraction teachnique

中图分类号:

TQ94

姜贵全;方桂珍;庞久寅. 地榆根提取低聚原花青素的研究[J]. 林产化学与工业, 2007, 27(S1): 129-132.

JIANG Gui-quan;FANG Gui-zhen;PANG Jiu-yin. Study on Extraction of Oligoprocyanidins from the Root of Sanguisorba officinalis Linn.[J]. , 2007, 27(S1): 129-132.

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