红外光谱技术在槐米总黄酮提取过程中的应用

林产化学与工业 ›› 2007, Vol. 27 ›› Issue (S1): 60-64.

红外光谱技术在槐米总黄酮提取过程中的应用

张黎明¹, 赵希¹, 高文远²

1. 天津科技大学生物工程学院工业微生物重点实验室, 天津 300457;2. 天津大学药物科学与生命技术学院, 天津 300072

收稿日期:2007-05-18 修回日期:1900-01-01 出版日期:2007-10-20 发布日期:2007-10-20

Extraction and FT-IR Spectral Identification of Flavonoids Extracted from the Buds of Sophora japonica Linn.

ZHANG Li-ming¹, ZHAO Xi¹, GAO Wen-yuan²

1. Tianjin Key Laboratory of Industrial Microbiology, College of Biotechnology, Tianjin University of Science & Technology, Tianjin 300457, China;2. School of Pharmaceutical Science and Technology, Tianjin University, Tianjin 300072, China

Received:2007-05-18 Revised:1900-01-01 Online:2007-10-20 Published:2007-10-20

摘要/Abstract

摘要： 以芦丁标准品作对照,应用分光光度法和红外光谱法(FT-IR)对碱提酸沉法、乙醇回流法和超声波辅助法3种不同提取方法制备的槐米总黄酮提取物进行分析.结果表明,利用碱提酸沉法提取,碱提取液pH值9及硼砂作稳定剂时,所得提取物的得率和总黄酮含量均较高,分别为19.44%和82.53%;由于提取方法和条件的不同,对应提取物红外光谱的特征峰型、峰高和峰位置存在一定的差异,通过分析提取物红外图谱特征,并与芦丁标准品比较,将为采用定量分析方法结合红外光谱技术控制槐米总黄酮提取物的质量提供参考依据.

关键词: 槐米, 黄酮类化合物, 提取物, 芦丁

Abstract: Flavonoids from the buds of Sophora japonica Linn. were extracted by three different methods, namely alkali extraction followed by acid sedimentation, ethanol refluxing and ultrasound-assisted extraction. The extraction conditions, including pH value of solvent and the kind of stabilizer were researched as well. Furthermore, with rutin as comparison, the flavonoids content of different extracts from buds of Sophora japonica L. and their FT-IR spectra had been determined. The results showed that both the yield and purity of the products by alkali extracting followed by acid sedimentation with the extract adjusted to pH value 9 containing borax as stabilizer were higher than others. Meanwhile, the typical FT-IR spectra of every sample had specific absorption peak pattern, intensity and location, related to different extracting technology. Among them, the FT-IR spectrum of flavonoids obtained under the above optimum extraction technology was similar to that of standard rutin. Therefore, quantitative analysis along with their FT-IR spectra provide a favorable foundation for quality control of flavonoids from the buds of Sophora japonica Linn..

Key words: buds of Sophora japonica Linn., flavonoids, extracts, rutin

中图分类号:

TQ91

张黎明;赵希;高文远. 红外光谱技术在槐米总黄酮提取过程中的应用[J]. 林产化学与工业, 2007, 27(S1): 60-64.

ZHANG Li-ming;ZHAO Xi;GAO Wen-yuan. Extraction and FT-IR Spectral Identification of Flavonoids Extracted from the Buds of Sophora japonica Linn.[J]. , 2007, 27(S1): 60-64.

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