水稻秸秆降解优良放线菌的筛选和鉴定

林产化学与工业 ›› 2008, Vol. 28 ›› Issue (5): 55-59.

水稻秸秆降解优良放线菌的筛选和鉴定

徐杰, 杨谦

哈尔滨工业大学生命科学与工程系, 黑龙江, 哈尔滨, 15000

收稿日期:2007-11-21 修回日期:1900-01-01 出版日期:2008-10-30 发布日期:2008-10-30

Isolation and Identification of An Effective Actinomycete Strain for Degradating Rice Straw

XU Jie, YANG Qian

Department of Life Science and Engineering, Harbin Institute of Technology, Harbin 150001, China

Received:2007-11-21 Revised:1900-01-01 Online:2008-10-30 Published:2008-10-30

摘要/Abstract

摘要： 利用纤维素琼脂选择培养基和刚果红染色方法,从常年堆放的水稻秸秆垛下面的新鲜土壤及腐烂秸秆中筛选分离获得一株具有较高纤维素酶活菌株,经培养特征观察和16S rRNA 序列分析鉴定为链霉菌C-5.该菌株同时具有一定的漆酶、过氧化物酶、木聚糖酶和果胶酶活性,并且能在30d内使水稻秸秆的干物质失重率达72%以上,具有很好的水稻秸秆降解能力,对提高水稻秸秆的资源利用率有良好的应用前景.

关键词: 水稻秸秆分解菌, 链霉菌, 16S rRNA序列, 测序

Abstract: An actinomycete strain C-5, capable of degradating rice straw effectively, was isolated from soil which had been under the stock pile for several years, using methods of cellulose culture-medium selection and Congo red dyeing. The isolated strain was identified as Streptomyces sp. according to its phenotypic features and 16S rRNA phylogenetic analysis. This strain has enzymatic activities of cellulase, laccase, peroxidase, xylanase and pectinase simultaneously. After 30d of inoculating with C-5, the dry weigh of rice straw was decreased by 72%. C-5 could decompose rice straw effectively and had good application prospects in improving utilization of the rice straw resource.

Key words: actinomycete strain for rice-straw degradation, streptomycete, 16S rRNA sequence, sequencing

中图分类号:

TQ351.0

徐杰;杨谦. 水稻秸秆降解优良放线菌的筛选和鉴定[J]. 林产化学与工业, 2008, 28(5): 55-59.

XU Jie;YANG Qian. Isolation and Identification of An Effective Actinomycete Strain for Degradating Rice Straw[J]. , 2008, 28(5): 55-59.

[1]	闫巧娟;翟倩;江正强. 链霉菌Z18中一种木聚糖酶(XynA)的纯化和性质[J]. 林产化学与工业, 2009, 29(4): 17-22.
[2]	蔡燕飞;李华兴;彭桂香;刘远金;蔡爱华;赵肃清. 纤维素分解菌的筛选及鉴定[J]. 林产化学与工业, 2005, 25(2): 67-70.