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林产化学与工业 ›› 2010, Vol. 30 ›› Issue (3): 53-56.

• 研究报告 • 上一篇    下一篇

水酶法提取辣木油的工艺研究

马李一1, 余建兴2, 张重权1, 王有琼1, 甘瑾1, 郑华1, 郭元亨1, 赵虹1, 段琼芬1   

  1. 1. 中国林业科学研究院 资源昆虫研究所, 云南 昆明 650224;2. 昆明医学院 公共卫生学院, 云南 昆明 650031
  • 收稿日期:2009-06-26 修回日期:1900-01-01 出版日期:2010-06-30 发布日期:2010-06-30
  • 通讯作者: 段琼芬,副研究员,博士,主要从事天然产物的开发利用研究;E-mail:qiongfend@hotmail.com。

Aqueous Enzymatic Extraction of Moringa oleifera Lam.Seed Oil

MA Li-yi1, YU Jian-xing2, ZHANG Zhong-quan1, WANG You-qiong1, GAN Jin1, ZHENG Hua1, GUO Yuan-heng1, ZHAO Hong1, DUAN Qiong-fen1   

  1. 1. Research Institute of Resource Insects, Chinese Academy of Forestry, Kunming 650224, China;2. School of Public Health, Kunming Medical University, Kunming 650031, China
  • Received:2009-06-26 Revised:1900-01-01 Online:2010-06-30 Published:2010-06-30

摘要: 通过中性蛋白酶水相溶剂中提取辣木油的单因素和正交试验,研究了影响辣木油水酶法提取的主要因素,得出了水酶法提取辣木油的最佳工艺条件。研究结果表明,最佳条件为:酶用量为辣木籽质量的3.0%,液料比9:1(mL:g),温度50℃,孵育时间18h,pH值5.0的条件下,辣木油平均得率超过28.6%。

关键词: 辣木油, 提取方法, 中性蛋白酶, 水酶法

Abstract: Through single factors and orthogonal experiments, the optimal condition for aqueous enzymatic extraction of Moringa oleifera Lam.seed oil with neutral protease was studied. The optimal condition is as follows: amount of neutral protease 3% of M.oleifera seed, ratio of enzyme solution to M.oleifera seed 9:1, incubated at 50℃ at pH value 5 for 18h, average oil yield exceeded 28.25%.

Key words: Moringa oleifera seed oil, extraction methods, neutral protease, aqueous enzymatic extraction

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