离子液体在天然活性成分分离中的应用研究进展

doi:10.3969/j.issn.0253-2417.2019.06.001

摘要/Abstract

摘要：

离子液体是由阴、阳离子组成的低温熔融盐，几乎没有蒸汽压，稳定性好，溶解能力强，结构可设计，优点突出，被认为是可替代传统溶剂的新型"绿色溶剂"，在天然产物的提取分离研究中应用广泛。本文从离子液体联用超声波、微波、双水相萃取、酶法辅助与固相萃取等方面，综述了近年来离子液体在黄酮、萜类、生物碱、色素等天然成分提取分离中的应用情况，介绍了离子液体与其它技术联用的性质和分离机制，探讨了离子液体选择性差、黏度高、回收难及流失严重等问题，并提出了相应的解决思路，以期为离子液体在天然活性成分分离方面的应用提供借鉴和参考。

关键词: 离子液体, 天然产物, 超声波辅助萃取, 微波辅助萃取, 离子液体双水相萃取

Abstract:

Ionic liquid is a low-temperature molten salt composed of anion and cation. It has almost no vapor pressure, good chemical stability, strong solubility, designable structure and outstanding advantages. It is considered as a new type of "green solvent" that can replace traditional solvent. Ionic liquid has been extensively applied in the separation and purification of natural products. In this paper, the application progress of ionic liquids in the separation of flavonoids, terpenes, alkaloids, pigments and other natural products were reviewed from the aspects of ionic liquids combined with ultrasonic and microwave technology, aqueous two-phase extraction, enzymatic assisted and solid-phase extraction in recent years. The properties and separation mechanism of ionic liquids combined with other technologies were introduced. The problems such as high viscosity, difficult recovery and serious loss, would be discussed, and the corresponding solutions would be put forward to provide reference for the application of ionic liquids in the separation of natural components.

Key words: ionic liquids, natural products, ultrasonic-assisted extraction, microwave-assisted extraction, ionic liquids aqueous two-phase extraction

中图分类号:

TQ35
TQ91

刘磊磊,王艺聪. 离子液体在天然活性成分分离中的应用研究进展[J]. 林产化学与工业, 2019, 39(6): 1-12.

Leilei LIU,Yicong WANG. Research Progress in Application of Ionic Liquid in Extraction and Separation of Natural Products[J]. Chemistry and Industry of Forest Products, 2019, 39(6): 1-12.

图/表 5

图1

图2

图3

图4

图5

参考文献 61

1	魏金凤, 张志娟, 李冬冬, 等. 离子液体辅助萃取山高粱中4种黄酮类成分的研究[J]. 中国中药杂志, 2015, 40 (7): 1305- 1310.
	WEI J F , ZHANG Z J , LI D D , et al. Analysis of four flavonoids in Lysimachia clethroids using ionic liquid-assisted extraction[J]. China Journal of Chinese Materia Medica, 2015, 40 (7): 1305- 1310.
2	WEI J F , CAO P R , WANG J M , et al. Analysis of tilianin and acacetin in Agastache rugosa by high-performance liquid chromatography with ionic liquids-ultrasound based extraction[J]. Chemical Engineering Journal, 2016, 10, 76- 85. doi: 10.1186/s13065-016-0223-7
3	LI S L , LI S N , HUANG Y , et al. Ionic-liquid-based ultrasound-assisted extraction of isoflavones from Belamcanda chinensis and subsequent screening and isolation of potential α-glucosidase inhibitors by ultrafiltration and semipreparative high-performance liquid chromatography[J]. Journal of Separation Science, 2017, 40 (12): 2565- 2574. doi: 10.1002/jssc.201700258
4	LI W J , SHI M J , WANG P Y , et al. Efficient deter mination of three flavonoids in Malus pumila flowers by ionic liquid-HPLC[J]. Journal of Molecular Liquids, 2018, 263, 139- 146. doi: 10.1016/j.molliq.2018.04.135
5	HARDE S M , LONKAR S L , DEGANI M S , et al. Ionic liquid based ultrasonic-assisted extraction of forskolin from Coleus forskohlii roots[J]. Industrial Crops and Products, 2014, 61, 258- 264. doi: 10.1016/j.indcrop.2014.07.016
6	刘微, 李冬冬, 杨洪帅, 等. 超声辅助离子液体-反相液相色谱法测定赤芍中齐墩果酸和芍药苷的含量[J]. 中国中药杂志, 2015, 40 (3): 443- 449.
	LIU W , LI D D , YANG H S , et al. Deter mination of oleanic acid and paeoniflorin in Paeonia lactiflora by ultrasound-assisted ionic liquid-reversed phase liquid chromatography[J]. China Journal of Chinese Materia Medica, 2015, 40 (3): 443- 449.
7	WANG Z B , CAO B C , YU A M , et al. Ultrasound-assisted ionic liquid-based homogeneous liquid-liquid microextraction high-performance liquid chromatography for deter mination of tanshinones in Salvia miltiorrhizaBge.Root[J]. Journal of Pharmaceutical and Biomedical Analysis, 2015, 104, 97- 104. doi: 10.1016/j.jpba.2014.11.034
8	张小梅, 陈一龙, 姚媛媛, 等. 基于离子液体-超声辅助-HPLC同时测定昆明山海棠中5种成分的含量[J]. 中国中药杂志, 2016, 41 (5): 879- 886.
	ZHANG X M , CHEN Y L , YAO Y Y , et al. Simultaneous deter mination of 5 compounds in Tripterygium hypoglaucum based on ultrasound-assisted ionic liquid coupled with HPLC[J]. China Journal of Chinese Materia Medica, 2016, 41 (5): 879- 886.
9	WANG W C , LI Q Y , LIU Y H , et al. Ionic liquid-aqueous solution ultrasonic-assisted extraction of three kinds of alkaloids from Phellodendron amurense Rupr and optimize conditions use response surface[J]. Ultrasonics Sonochemistry, 2015, 24, 13- 18. doi: 10.1016/j.ultsonch.2014.10.009
10	CAO X J , ZHU C L , WANG L P , et al. Investigating acidic ionic liquid-based ultrasonic-assisted extraction of leonurine from Herba Leonuri[J]. Separation Science and Technology, 2018, 53 (3): 481- 486. doi: 10.1080/01496395.2017.1385628
11	LI L Q , HUANG M Y , SHAO J L , et al. Rapid deter mination of alkaloids in Macleaya cordata using ionic liquid extraction followed by multiple reaction monitoring UPLC-MS/MS analysis[J]. Journal of Pharmaceutical and Biomedical Analysis, 2017, 135, 61- 66. doi: 10.1016/j.jpba.2016.12.016
12	QIN H Y , ZHOU G M , PENG G L , et al. Application of ionic liquid-based ultrasound-assisted extraction of five phenolic compounds from Fig (Ficus carica L.) for HPLC-UV[J]. Food Analytical Methods, 2015, 8 (7): 1673- 1681. doi: 10.1007/s12161-014-0047-9
13	XU J L , WANG W C , LIANG H , et al. Optimization of ionic liquid based ultrasonic assisted extraction of antioxidant compounds from Curcuma longa L.using response surface methodology[J]. Industrial Crops and Products, 2015, 76, 487- 493. doi: 10.1016/j.indcrop.2015.07.025
14	杨红帅, 王菊, 郭翠, 等. 超声辅助离子液体-反相液相色谱法同时测定茜草中茜草素和大叶茜草素的含量[J]. 中国中药杂志, 2015, 40 (13): 2617- 2627.
	YANG H S , WANG J , GUO C , et al. Simultaneous deter mination of alizarin and rubimaillin in Rubia cordifolia by ultrasound-assistedionic liquid-reversed phase liquid chromatography[J]. China Journal of Chinese Materia Medica, 2015, 40 (13): 2617- 2627.
15	SUN Y S , HOU Z G , LIU Z B , et al. Ionic liquid-based ultrasonic-assisted extraction of Forsythosides from the leaf of Forsythia suspensa (Thunb.) Vahl and subsequent separation and purification by high-speed counter-current chromatography[J]. Journal of Chromatographic Science, 2016, 54 (8): 1445- 1452. doi: 10.1093/chromsci/bmw069
16	ULLAH H , CECILIA D W , MAIZATUL S S . Ionic liquid based ultrasonication-assisted extraction of essential oil from the leaves of Persicaria minor and conductor-like screening model for realistic solvents study[J]. Chinese Journal of Chromatography, 2017, 35 (6): 656- 664. doi: 10.3724/SP.J.1123.2017.02002
17	ZHANG Y F , LIU Z , LI Y L , et al. Optimization of ionic liquid-based microwave-assisted extraction of isoflavones from Radix puerariae by response surface methodology[J]. Separation and Purification Technology, 2014, 129, 71- 79. doi: 10.1016/j.seppur.2014.03.022
18	ZHOU Y , WU D T , CAI P F , et al. Special effect of ionic liquids on the extraction of flavonoid glycosides from Chrysanthemum morifolium Ramat by microwave assistance[J]. Molecules, 2015, 20 (5): 7683- 7699. doi: 10.3390/molecules20057683
19	ZHANG Q , ZHAO S H , CHEN J , et al. Application of ionic liquid-based microwave-assisted extraction of flavonoids from Scutellaria baicalensis Georgi[J]. Journal of Chromatography B, 2015, 1002, 411- 417. doi: 10.1016/j.jchromb.2015.08.021
20	GU H Y , CHEN F L , ZHANG Q , et al. Application of ionic liquids in vacuum microwave-assisted extraction followed by macroporous resin isolation of three flavonoids rutin, hyperoside and hesperidin from Sorbus tianschanica leaves[J]. Journal of Chromatography B, 2016, 1014, 45- 55. doi: 10.1016/j.jchromb.2016.01.045
21	LI D , QIAN Y , TIAN Y J , et al. Optimization of ionic liquid-assisted extraction of biflavonoids from Selaginella doederleinii and evaluation of its antioxidant and antitumor activity[J]. Molecules, 2017, 22 (4): 586- 602. doi: 10.3390/molecules22040586
22	ZHAO C J , LU Z C , LI C Y , et al. Optimization of ionic liquid based simultaneous ultrasonic- and microwave-assisted extraction of rutin and quercetin from leaves of velvetleaf (Abutilon theophrasti) by response surface methodology[J]. The Scientific World Journal, 2014, 2014, 1- 11.
23	WANG Z B , HU J X , DU H X , et al. Microwave-assisted ionic liquid homogeneous liquid-liquid microextraction coupled with high performance liquid chromatography for the deter mination of anthraquinones in Rheum palmatum L[J]. Journal of Pharmaceutical and Biomedical Analysis, 2016, 125, 178- 185. doi: 10.1016/j.jpba.2016.03.046
24	LI S Y , CHEN F L , JIA J , et al. Ionic liquid-mediated microwave-assisted simultaneous extraction and distillation of gallic acid, ellagic acid and essential oil from the leaves of Eucalyptus camaldulensis[J]. Separation and Purification Technology, 2016, 168, 8- 18. doi: 10.1016/j.seppur.2016.05.013
25	BHAN M , SATIJA S , GARG C , et al. Optimization of ionic liquid-based microwave assisted extraction of a diterpenoid lactone-andrographolide from Andrographis paniculata by response surface methodology[J]. Journal of Molecular Liquids, 2017, 229, 161- 166. doi: 10.1016/j.molliq.2016.12.011
26	GUO J B , FAN Y , ZHANG W J , et al. Extraction of gingerols and shogaols from ginger (Zingiber officinale Roscoe) through microwave technique using ionic liquids[J]. Journal of Food Composition and Analysis, 2017, 62, 35- 42. doi: 10.1016/j.jfca.2017.04.014
27	FAN Y C , XUE C , LI J , et al. Ionic liquid-based microwave-assisted extraction of verbascoside from Rehmannia root[J]. Industrial Crops and Products, 2018, 124, 59- 65. doi: 10.1016/j.indcrop.2018.07.063
28	LIU Z Z , GU H Y , YANG L . An approach of ionic liquids/lithium salts based microwave irradiation pretreatment followed by ultrasound-microwave synergistic extraction for two coumarins preparation from Cortex fraxini[J]. Journal of Chromatography A, 2015, 1417, 8- 20. doi: 10.1016/j.chroma.2015.09.037
29	TAN Z J , WANG C Y , YANG Z Z , et al. Ionic liquid-based ultrasonic-assisted extraction of secoisolariciresinol diglucoside from Flaxseed (Linum usitatissimum L.) with further purification by an aqueous two-phase system[J]. Molecules, 2015, 20 (10): 71929- 17943.
30	TAN T , LAI C J S , OUYANG H , et al. Ionic liquid-based ultrasound-assisted extraction and aqueous two-phase system for analysis of caffeoylquinic acids from FlosLonicerae Japonicae[J]. Journal of Pharmaceutical and Biomedical Analysis, 2016, 120, 134- 141. doi: 10.1016/j.jpba.2015.12.019
31	YANG Z Z , TAN Z J , LI F F , et al. An effective method for the extraction and purification of chlorogenic acid from ramie (Boehmeria nivea L.) leaves using acidic ionic liquids[J]. Industrial Crops and Products, 2016, 89, 78- 86. doi: 10.1016/j.indcrop.2016.05.006
32	TAN Z J , YI Y J , WANG H Y , et al. Extraction, preconcentration and isolation of flavonoids from Apocynum venetum L.leaves using ionic liquid-based ultrasonic-assisted extraction coupled with an aqueous biphasic system[J]. Molecules, 2016, 21 (3): 262- 273. doi: 10.3390/molecules21030262
33	WANG R P , CHANG Y N , TAN Z Z , et al. Applications of choline a mino acid ionic liquid in extraction and separation of flavonoids and pectin from ponkan peels[J]. Separation Science and Technology, 2016, 51 (7): 1093- 1102. doi: 10.1080/01496395.2016.1143006
34	LIMA Á S , SOARES C M F , PALTRAM R , et al. Extraction and consecutive purification of anthocyanins from grape pomace using ionic liquid solutions[J]. Fluid Phase Equilibria, 2017, 451, 68- 78. doi: 10.1016/j.fluid.2017.08.006
35	SHU Y , GAO M C , WANG X Y , et al. Separation of curcu minoids using ionic liquid based aqueous two-phase system coupled with in situ dispersive liquid-liquid microextraction[J]. Talanta, 2016, 149, 6- 12. doi: 10.1016/j.talanta.2015.11.009
36	WANG X Z , LI X W , LI L J , et al. Green deter mination of aconitum alkaloids in Aconitum carmichaeli (Fuzi) by an ionic liquid aqueous two-phase system and recovery of the ionic liquid coupled with in situ liquid-liquid microextraction[J]. Analytical Methods, 2016, 8, 6566- 6572. doi: 10.1039/C6AY01139J
37	GE L , XIA F , SONG Y , et al. Solubility of luteolin in several imidazole-based ionic liquids and extraction from peanut shells using selected ionic liquid as solvent[J]. Separation and Purification Technology, 2014, 135, 223- 228. doi: 10.1016/j.seppur.2014.08.022
38	ĆURKO N , TOMAŠEVIĆ M , CVJETKO BUBALO M , et al. Extraction of proanthocyanidins and anthocyanins from grape skin by using ionic liquids[J]. Food Science and Biotechnology, 2017, 55 (3): 429- 437.
39	STREHMEL N , STRUNK D , STREHME V . Extraction of natural products from bark of Betula pendula using ionic liquids[J]. Metabolomics, 2017, 13, 135- 143. doi: 10.1007/s11306-017-1271-2
40	LAU B B Y , PANCHOMPOO J , ALDOUS L . Extraction and electrochemical detection of capsaicin and ascorbic acid from fresh chilli using ionic liquids[J]. New Journal of Chemistry, 2015, 39 (2): 860- 867. doi: 10.1039/C4NJ01416B
41	DONG B , TANG J , YONANNES A , et al. Hexafluorophosphate salts with tropine-type cations in the extraction of alkaloids with the same nucleus from Radix physochlainae[J]. RSC Advances, 2018, 8, 262- 277. doi: 10.1039/C7RA12687E
42	AMINKAR S , SHOJAEIYAN A , AYYARI M . Enhancement of diosgenin extraction from Fenugreek (Trigonella foenum-graecum) with a new ionic liquid-based assisted method[J]. Planta Medica, 2016, 82 (S01): S1- S381. doi: 10.1055/s-0036-1596797
43	CLÁUDIO A F M , COGNIGNI A , FARIA E , et al. Valorization of olive tree leaves:Extraction of oleanolic acid using aqueous solutions of surface-active ionic liquids[J]. Separation and Purification Technology, 2018, 204, 30- 37. doi: 10.1016/j.seppur.2018.04.042
44	LIU T T , SUI X Y , LI L , et al. Application of ionic liquids based enzyme-assisted extraction of chlorogenic acid from Eucommia ulmoides leaves[J]. Analytica Chimica Acta, 2016, 903, 91- 99. doi: 10.1016/j.aca.2015.11.029
45	SUN Y , DING S , HUANG H , et al. Ionic liquid-based enzyme-assisted extraction of chlorogenic acid from Flos Lonicera japonicae[J]. Bioresources and Bioprocessing, 2017, 4, 45- 53. doi: 10.1186/s40643-017-0175-3
46	SAHNE F , MOHAMMADI M , NAJAFPOUR G D , et al. Enzyme-assisted ionic liquid extraction of bioactive compound from turmeric (Curcuma longa L.):Isolation, purification and analysis of curcu min[J]. Industrial Crops and Products, 2017, 95, 686- 694. doi: 10.1016/j.indcrop.2016.11.037
47	CUI G Q , YANG X B , LIU T T , et al. An efficient approach for the enzyme-enhanced extraction of camptothecin and 10-hydroxycamptothecin from the samara of Camptotheca acu minata using an ionic liquid solution[J]. Separation and Purification Technology, 2018, 200, 102- 111. doi: 10.1016/j.seppur.2018.02.030
48	CHEN G , SUI X Y , LIU T T , et al. Application of cellulase treatment in ionic liquid based enzyme-assisted extraction in combine with in-situ hydrolysis process for obtaining genipin from Eucommia ulmoides Olive barks[J]. Journal of Chromatography A, 2018, 1569, 26- 35. doi: 10.1016/j.chroma.2018.07.063
49	XU J J , YANG R , YE L H , et al. Aplication of ionic liquids for elution of bioactive flavonoid glycosides from lime fruit by miniaturized matrix solid-phase dispersion[J]. Food Chemistry, 2016, 204, 167- 175. doi: 10.1016/j.foodchem.2016.02.012
50	LI G Z , AHN W S , ROW K H . Hybrid molecularly imprinted polymers modified by deep eutectic solvents and ionic liquids with three templates for the rapid simultaneous purification of rutin, scoparone and quercetin from Herba artemisiae Scopariae[J]. Journal of Separation Science, 2016, 39 (23): 4465- 4473. doi: 10.1002/jssc.201600892
51	CHEN F L , ZHANG Q , SHIMIN K M , et al. Optimization of ionic liquid-based homogenate extraction of orientin and vitexin from the flowers of Trollius chinensis and its application on a pilot scale[J]. Separation and Purification Technology, 2017, 175, 147- 157. doi: 10.1016/j.seppur.2016.10.062
52	LIU Z Z , GU H Y , YANG L . A novel approach for the simultaneous extraction of dihydroquercetin and arabinogalactan from Larix gmelinii by homogenate-ultrasound-synergistic technique using the ionic liquid[J]. Journal of Molecular Liquids, 2018, 261, 41- 49. doi: 10.1016/j.molliq.2018.04.011
53	ZHANG W , FENG X T , ALULA Y , et al. Bionic multi-tentacled ionic liquid-modified silica gel for adsorption and separation of polyphenols from green tea (Camellia sinensis) leaves[J]. Food Chemistry, 2017, 230, 637- 648. doi: 10.1016/j.foodchem.2017.03.054
54	FENG X T , ZHANG W , ZHANG T H , et al. Systematic investigation for extraction and separation of polyphenols in tea leaves by magnetic ionic liquids[J]. Journal of the Science of Food and Agriculture, 2018, 98 (12): 4550- 4560. doi: 10.1002/jsfa.8983
55	LI F J , YUAN Y , LI H , et al. Infrared-assisted extraction of salidroside from the root of Rhodiola crenulata with a novel ionic liquid that dissolves cellulose[J]. RSC Advances, 2015, 5 (59): 47326- 47333. doi: 10.1039/C5RA07969A
56	LI G Z , WANG W , WANG Q , et al. Deep eutectic solvents modified molecular imprinted polymers for optimized purification of chlorogenic acid from honeysuckle[J]. Journal of Chromatographic Science, 2016, 54 (2): 271- 279.
57	SHEIKHIAN L , BIN S . Simultaneous extraction and HPLC deter mination of 3-indole butyric acid and 3-indole acetic acid in pea plant by using ionic liquid-modified silica as sorbent[J]. Journal of Chromatography B, 2016, 1009, 34- 43. doi: 10.1016/j.jchromb.2015.11.047
58	LI G Z , WANG X Q , ROW K H , et al. Magnetic solid-phase extraction with Fe₃O₄/molecularly imprinted polymers modified by deep eutectic solvents and ionic liquids for the rapid purification of alkaloid isomers (theobro mine and theophylline) from green tea[J]. Molecules, 2017, 22 (7): 1061- 1075. doi: 10.3390/molecules22071061
59	FARIA D , EMANUELLE L P , GOMES M V , et al. Extraction and recovery processes for cynaropicrin from Cynara cardunculus L.using aqueous solutions of surface-active ionic liquids[J]. Biophysical Reviews, 2018, 10 (3): 915- 925. doi: 10.1007/s12551-017-0387-y
60	ZHANG H , ROW K H . Application of ionic liquid modified silica for solid-phase extraction of polysaccharides from Laminaria japonica[J]. Journal of Carbohydrate Chemistry, 2014, 33 (5): 225- 237. doi: 10.1080/07328303.2014.913059
61	XU J J , LI Q , CAO J , et al. Extraction and enrichment of natural pigments from solid samples using ionic liquids and chitosan nanoparticles[J]. Journal of Chromatography A, 2016, 1463, 32- 41. doi: 10.1016/j.chroma.2016.08.012

[1]	程逸超, 杨金龙, 朱均均, 徐勇, 勇强, 余世袁. 疏水性离子液体[C₄mim][PF₆]对木质纤维预水解液的脱毒作用[J]. 林产化学与工业, 2017, 37(5): 45-52.
[2]	曹菲, 赵鑫, 胡英成, 陈培培, 李慧芳. 基于离子液体的椰壳纤维纳米纤维素的制备与表征[J]. 林产化学与工业, 2017, 37(5): 139-145.
[3]	王兰英, 庄军平, 周彦斌, 武书彬. 毛竹纤维在1-丁基-3-甲基咪唑氯盐/乙醇中的溶解特性研究[J]. 林产化学与工业, 2017, 37(4): 110-114.
[4]	吴鸿楼, 谌凡更, 王静. 桉木屑在离子液体[C₄H₈SO₃Hmim]HSO₄催化下液化行为研究[J]. 林产化学与工业, 2017, 37(2): 42-48.
[5]	陈莉晶, 左宋林, 张秋红, 陈继锡, 王永芳. 二次水蒸气活化制备离子液体超级电容器活性炭的研究[J]. 林产化学与工业, 2017, 37(1): 87-93.
[6]	王亚男, 刘仕伟, 李露, 于世涛. 木质素在三氮唑基离子液体中溶解性能的研究[J]. 林产化学与工业, 2016, 36(5): 45-52.
[7]	崔晶晶, 李云华, 陈秉辉. 离子液体[Hmim][CF₃SO₃]在木质素及其模型化合物生物降解中的应用[J]. 林产化学与工业, 2016, 36(4): 1-6.
[8]	李凤凤, 陈嘉川, 庞志强, 杨桂花, 张志礼. 离子液体预处理对纸浆酶漂效果的研究[J]. 林产化学与工业, 2015, 35(4): 79-84.
[9]	李利芬, 胡英成. 离子液体在木质素研究中的应用[J]. 林产化学与工业, 2015, 35(2): 163-170.
[10]	钟黎黎, 高源, 张力平. cellulose-g-PMMA的合成及其胶束作为药物载体的研究[J]. 林产化学与工业, 2015, 35(1): 23-29.
[11]	刘仕伟, 周红霞, 李露, 于世涛, 解从霞, 刘福胜. 微波辅助离子液体溶解木质素的研究[J]. 林产化学与工业, 2014, 34(5): 41-46.
[12]	刘先红, 李露, 于世涛, 刘仕伟, 刘福胜, 解从霞. 离子液体促进酶原位降解天然植物纤维的研究[J]. 林产化学与工业, 2014, 34(4): 25-29.
[13]	裴立军, 蔡照胜, 商士斌, 宋湛谦. 碱性离子液体条件下烷基化壳聚糖衍生物的制备及其抑菌性能研究[J]. 林产化学与工业, 2014, 34(3): 44-50.
[14]	郑怀玉, 卢泽湘, 范立维, 黄彪, 廖益强, 胡阳. [Psmim]HSO₄离子液体催化液化木材及其产物表征[J]. 林产化学与工业, 2014, 34(2): 91-96.
[15]	杨杰;张金生;李丽华;赵文涛;蔚永清. 响应面法优化微波辅助提取丝瓜籽油的研究[J]. 林产化学与工业, 2013, 33(4): 128-132.