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林产化学与工业

林产化学与工业 ›› 2011, Vol. 31 ›› Issue (4): 75-78.

• 研究报告 • 上一篇    下一篇

紫外分光光度法测定白千层枝和叶总三萜酸含量

范超君, 鲍长余, 毕和平, 陈湛娟   

  1. 海南师范大学 省部共建热带药用植物化学教育部重点实验室; 化学与化工学院, 海南 海口 571127
  • 收稿日期:2011-04-06 修回日期:1900-01-01 出版日期:2011-08-30 发布日期:2011-08-30
  • 通讯作者: 毕和平,教授,主要从事天然药物化学研究工作;E-mail:bhphx@hainnu.edu.cn。

Ultra-violet Spectrophotometric Determination of Triterpenoidic Acid in the Stems and Leaves of Melaleuca leucadendra

FAN Chao-jun, BAO Chang-yu, BI He-ping, CHEN Zhan-juan   

  1. Key Laboratory of Tropical Medicinal Plant Chemistry of Ministry of Education;College of Chemistry and Chemical Engineering, Hainan Normal University, Haikou 571127, China
  • Received:2011-04-06 Revised:1900-01-01 Online:2011-08-30 Published:2011-08-30

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摘要： 以 5 %香草醛-冰乙酸和高氯酸为显色剂,测定白千层枝和叶中三萜酸的总含量。熊果酸对照品在0.20~1.60 mg/L 范围内呈良好线性关系,其回归方程为A=0.154 8C+0.028 6,r=0.999 3。结果表明白千层枝中三萜酸的总质量分数约为 1.096 %,叶中三萜酸的总质量分数约为 1.017 %。

关键词: 白千层, 三萜酸, 紫外分光光度法

Abstract: The total content of triterpenoidic acid in the stems and leaves of Melaleuca leucadendra Linn.was determined using 5 % vanillin-acetic acid and perchloric acid as color system. Ursolic acid had a better linear relationship in the range of 0.20-1.60 mg/L and its regression equation was A=0.154 8C+0.028 6(r=0.999 3).The results showed that the total mass fractions of triterpenoidic acid in the stems and leaves of M. leucadendra were 1.096 % and 1.017 % respectively.

Key words: Melaleuca leucadendra Linn., tritepenoidic acid, ultra-violet spectrophotometric method

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