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林产化学与工业 ›› 2011, Vol. 31 ›› Issue (4): 87-92.

• 研究报告 • 上一篇    下一篇

响应曲面法优化微波辅助提取胭脂虫红色素工艺

郭元亨, 马李一, 郑华, 张弘, 甘瑾, 李凯, 涂行浩   

  1. 中国林业科学研究院 资源昆虫研究所;国家林业局 资源昆虫培育与 利用重点实验室, 云南 昆明 650224
  • 收稿日期:2010-11-18 修回日期:1900-01-01 出版日期:2011-08-30 发布日期:2011-08-30
  • 通讯作者: 张 弘(1963-),男,研究员,主要从事林业生物资源化学与利用研究;E-mail:kmzhhong@163.com。

Optimizing Conditions for Microwave-assisted Extraction of Cochineal Dye Using Response Surface Method

GUO Yuan-heng, MA Li-yi, ZHENG Hua, ZHANG Hong, GAN Jin, LI Kai, TU Xing-hao   

  1. Research Institute of Resource Insects, Chinese Academy of Forestry;Key Laboratory of Cultivation and Utilization of Resource Insects, State Forestry Administration, Kunming 650224, China
  • Received:2010-11-18 Revised:1900-01-01 Online:2011-08-30 Published:2011-08-30

摘要: 利用微波辅助水提取胭脂虫红色素,通过单因素试验考察了提取条件对色素得率的影响。结果表明,5 g 胭脂虫干样,微波处理前常温浸泡 40 min,液料比7∶1(g∶mL),微波功率 700 W,辐射 60 s 后 75 ℃ 水浴浸提 40 min 适宜于色素提取。在单因素试验的基础上,进行3因素3水平响应面分析试验,得到最优条件为 5 g 胭脂虫干样,液料比值7.5,微波功率 714 W,辐射时间 73 s,色素得率可达 7.30 %。最优条件下,合适的提取次数为12次,色素总得率达到 29.95 %,提取率为 95.13 %。

关键词: 胭脂虫红色素, 微波, 提取, 响应面优化

Abstract: Cochineal (Dactylopius coccus Costa) dye was extracted by microwave-assisted extraction with water. Effects of single-factor test on the yield of cochineal dye was carried out. The results show that for 5 g cochineal, the suitable conditions for extraction of dye are cochineal sample soaked in water for 40min before microwave treatment, ratio of liquid to material 7∶1(g∶mL), microwave power 700 W, microwave treatment time 60 s, and extract with water-bath at 75 ℃ for 40 min after microwave treatment. According to the single-factor test, the microwave-assisted extraction process parameters for extraction of cochineal dye were optimized using response surface method. The results show that the optimum parameters should be as follows: cochineal 5 g, ratio of liquid to material 7.5, microwave power 714 W, microwave treatment time 73 s. The extraction yield of conchineal dye is up to 7.30%. Under this conditon the reasonable extracting times is 12, the total yield of conchineal dye is 29.95 % and extraction yield is 95.13 %.

Key words: cochineal dye, microwave, extraction, response surface method

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