欢迎访问《林产化学与工业》，
林产化学与工业

林产化学与工业 ›› 2008, Vol. 28 ›› Issue (3): 61-65.

• 论文 • 上一篇    下一篇

核磁共振光谱和X射线光电子能谱对纤维低聚糖的分析

彭红1,2, 林鹿1, 刘玉环2   

  1. 1. 华南理工大学制浆造纸工程国家重点实验室, 广东, 广州, 510640;2. 南昌大学, 教育部生物质转化工程技术研究中心, 江西, 南昌, 330047
  • 收稿日期:2007-08-23 修回日期:1900-01-01 出版日期:2008-06-30 发布日期:2008-06-30

Characterization of Cellooligosaccharides Using NMR Spectroscopy and X-ray Photoelectron Spectra

PENG Hong1,2, LIN Lu1, LIU Yu-huan2   

  1. 1. State Key Laboratory of Pulp & Paper Engineering, South China University of Technology, Guangzhou 510640, China;2. The Engineering Center for Biomass Refining, MOE, Nanchang University, Nanchang 330047, China
  • Received:2007-08-23 Revised:1900-01-01 Online:2008-06-30 Published:2008-06-30

PDF (PC)

572

摘要： 1H-1H COSY和13C-1H HSQC谱对通过Bio-Gel P-2柱(2.5 cm×12 cm)分离制备的纤维三糖、纤维四糖和纤维五糖进行分析.结果表明,在水溶液中纤维低聚糖还原末端C1位α构型与β构型之比(αβ)随聚合度增大而减小,纤维三糖主要是α构型(αβ,0.2),而纤维四糖和纤维五糖主要是β构型(αβ,分别是0.34和0.25).X射线光电子能谱(XPS)分析表明纤维低聚糖的C 1s主要有2种电子结合能,分别为284.8(C 1s1,C-O)和286.1 eV(C 1s2,O-C-O),C 1s1与C 1s2之比(C 1s1/C 1s2)随聚合度增大而增大.

关键词: 纤维低聚糖, 分离, 核磁共振光谱, X射线光电子能谱

Abstract: 1H-1H COSY and 13C-1H HSQC spectra were used to assign cellotriose, cellotetraose and cellopentaose preparatively separated on Bio-Gel P-2 column (2.5cm×125cm). The results illustrate that the configuration ratios of α-form to β-form (α/β) at C1 of the reducing end decrease with the increase of degree of polymerization (DP) for cellooligosaccharides in aqueous solution. The configuration for cellotriose is mainly the α-form (α/β, 0.52), while for cellotetraose and cellopentaose are mainly the β-form (α/β, 0.34 and 0.25 respectively). X-ray photoelectron spectra (XPS) analysis shows that the binding energy of C 1s was mainly assigned to 284.8 and 286.1 eV corresponding to C 1s1 (C—O) and C 1s2 (O—C—O), respectively. The ratios of C 1s1/C 1s2 increase with the increase of DP for cellooligosaccharides.

Key words: cellooligosaccharides, fractionation, NMR spectroscopy, X-ray photoelectron spectra

中图分类号: 

版权所有 © 中国林业科学研究院林产化学工业研究所　苏ICP备08107184号-3