欢迎访问《林产化学与工业》，
林产化学与工业

林产化学与工业 ›› 2007, Vol. 27 ›› Issue (3): 107-110.

• 研究报告 • 上一篇    下一篇

微波辅助萃取-固相微萃取联用技术分析桑叶挥发性成分

李杰红, 陈代武   

  1. 邵阳医学高等专科学校 药学系, 湖南 邵阳 422000
  • 收稿日期:2006-11-29 修回日期:1900-01-01 出版日期:2007-06-30 发布日期:2007-06-30

Analysis of Volatile Chemical Composition in Mulberry Leaves Using Microwave Assisted Extraction Associated with Solid Phase Microextraction

LI Jie-hong, CHEN Dai-wu   

  1. Department of Pharmacy, Shaoyang Medical College, Shaoyang 422000, China
  • Received:2006-11-29 Revised:1900-01-01 Online:2007-06-30 Published:2007-06-30

PDF (PC)

872

摘要： 用微波辅助萃取-固相微萃取联用技术,结合气相色谱-质谱法对桑叶挥发性成分进行了鉴定,并用面积归一化法测定其相对含量.共鉴定出化合物45种,包括酮、醇、酸、烷烃、烯烃、甾醇、醛、酯及杂环化合物等,其中酮类化合物占总色谱馏出峰面积的6.56%;醇类化合物占33.32%;酸类占13.71%;烃类占23.44%.主要化合物有:3,7,11,15-四甲基-2-十六醇(24.05%)、1-乙酰基--异丙基-二环[3.1.0]己烷(16.27%)、5-(2-异丁烯基)-,6,6-三甲基-3-环己烯酸(8.35%)、3,7,11,15-四甲基-2-十六碳烯-1-醇(5.67%).该技术可以简便、快捷、准确地进行桑叶挥发性成分的分析鉴定.

关键词: 微波辅助萃取-固相微萃取, 桑叶, 挥发性成分

Abstract: The volatile component in mulberry(Morus albs Linn.) leaves was obtained by microwave assisted extraction(MAE) associated with solid-phase microextraction(SPME), then separated and identified by gas chromatography-mass spectrometer(GC-MS).Forty-five compounds were identified. The main constituents of the extract from mulberry leaves were found to be ketones (6.56%),alcohols (33.32%),acids (13.71%), alkanes and alkenes(23.44%), sterols,aldehydes,esters and heterocyclic compounds. The main compounds were: 3,7,11,15-tetramethyl-2-hexadecanol (24.05%), 1-acetyl-4-isopropyl-bicyclo[3.1.0]hexane(16.27%),5-(2-isobutylenyl)-4,6,6-trimethyl-3-cyclohexenyl acid (8.35%),3,7,11,15-tetramethyl-2-hexadecen-1-o1(5.67%). MAE-SPME-GC-MS is convenient, rapid and reliable for analysis of volatile constituents in mulberry leaves.

Key words: microwave assisted extraction and solid-phase microextraction(MAE-SPME), mulberry leaves, volatile components

中图分类号: 

版权所有 © 中国林业科学研究院林产化学工业研究所　苏ICP备08107184号-3