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林产化学与工业

林产化学与工业 ›› 2007, Vol. 27 ›› Issue (S1): 105-108.

• 研究报告 • 上一篇    下一篇

竹醋液中酚类化合物的Folin-Ciocalteu法测定

钱华, 王衍彬, 许炯, 柏明娥   

  1. 浙江省林业科学研究院, 浙江 杭州 310023
  • 收稿日期:2007-06-20 修回日期:1900-01-01 出版日期:2007-10-20 发布日期:2007-10-20

Determination of the Content of Phenolic Compounds in Bamboo Vinegar by Folin-Ciocalteu's Method

QIAN Hua, WANG Yan-bing, XU Jiong, BO Ming-e   

  1. Zhejiang Forestry Academy, Hangzhou 310023, China
  • Received:2007-06-20 Revised:1900-01-01 Online:2007-10-20 Published:2007-10-20

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摘要： 以没食子酸丙酯为标准品研究了Folin-Ciocalteu法测定竹醋液中酚类化合物含量的适宜条件.结果表明,当Folin-Ciocalteu试剂与质量分数10%Na2CO3体积比1:1,反应温度35℃,反应时间120 min时,酚类化合物质量浓度在5～30 g/L范围内与吸光值呈良好的线性关系.测定方法的平均回收率为101.55%,相对标准偏差(RSD)为0.30%,有较好的准确度和精密度.

关键词: 竹醋液, 酚类, Folin-Ciocalteu's法

Abstract: Using propyl gallate as standard material, the optimum conditions for determining the content of phenolic compounds in bamboo vinegar by Folin-Ciocalteu's method were investigated. The results showed that the reaction conditions were:ratio of Folin reagent to 10% Na2CO3 1:1(volume ratio ),reaction temperature 35℃, reaction time 120 min. There was a good linearity in range of 5-30 g/L.The average recovery rate was 101.55% and the relative standard deviation (RSD) was 0.30%. This method had a good accuracy and precision to determine the content of phenolic compounds.

Key words: bamboo vinegar, phenolic compounds, Folin-Ciocalteu's method

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