硫酸对过氧化氢-乙酸预处理巨龙竹酶解和发酵的影响

doi:10.3969/j.issn.0253-2417.2023.05.015

摘要/Abstract

摘要：

以巨龙竹(DS)为研究对象，对其进行过氧化氢-乙酸(PA)预处理，探讨预处理中硫酸添加量对竹材化学组成、酶解效率和乙醇产率的影响。研究结果表明：随着预处理过程中硫酸用量的增加，竹材中的木质素质量分数不断降低，而纤维素和半纤维素的质量分数有所增加。在预处理中，添加硫酸作为反应助剂，能够使酶解效率提升，当硫酸体积分数为0.5%时，72 h的葡萄糖和木糖得率分别为84.77%和85.05%，与未添加硫酸的过氧化氢-乙酸预处理的巨龙竹相比，分别增加了42.40%和33.72%；其水解液经酵母发酵后的乙醇质量浓度为13.52 g/L，比未添加硫酸的过氧化氢-乙酸预处理水解液增加了73.33%。在巨龙竹过氧化氢-乙酸预处理过程中以硫酸作为助剂，能够降低底物的疏水性和表面木质素覆盖率，增加结晶度和比表面积，添加0.5%硫酸，纤维素酶可及度从未添加硫酸时的282.15 mg/g增加到500.12 mg/g，进而增加了纤维素酶与纤维素之间的生产性吸附，因此使巨龙竹的酶解效率和发酵产率得到提升。

关键词: 过氧化氢-乙酸预处理, 巨龙竹, 硫酸, 酶水解, 发酵

Abstract:

Taking Dendrocalamus sinicus(DS) as the research object, hydrogen peroxide-acetic acid(PA) pretreatment was carried out to investigate the effects of sulfuric acid addition on chemical compositions, enzymatic hydrolysis efficiency and ethanol yield. The results showed that the lignin content of bamboo decreased with the increase of sulfuric acid during PA pretreatment, while the relative contents of cellulose and hemicellulose increased. In addition, adding sulfuric acid as reaction promoter in PA pretreatment could improve the enzymatic hydrolysis efficiency. When the volume fraction of sulfuric acid was 0.5%, the 72 h yields of glucose and xylose were 84.77% and 85.05%, respectively, which were increased by 42.40% and 33.72% compared to that of hydrogen peroxide-acetic acid pretreatment without sulfuric acid. The hydrolysate was fermented by Saccharomyces cerevisiae and the mass concentration of ethanol was 13.52 g/L, which was 73.33% higher than that of the hydrolysate pretreated by hydrogen peroxide-acetic acid without sulfuric acid. During the PA pretreatment of D. sinicus, sulfuric acid was used as an additive, which could reduce the hydrophobicity of substrate and the surface coverage of lignin while the crystallinity and specific surface area were increased. Adding 0.5% sulfuric acid, the accessibility of cellulose increased from 282.15 mg/g (without sulfuric acid) to 500.12 mg/g, as well as further increased the productive adsorption between cellulase and cellulose, resulting in an improvement of the enzymatic hydrolysis efficiency and fermentation yield of D. sinicus.

Key words: hydrogen peroxide-acetic acid pretreatment, D. sinicus, sulfuric acid, enzymatic hydrolysis, fermentation

中图分类号:

TQ351.01

刘高源, 史正军, 赵平, 杨海艳, 杨静. 硫酸对过氧化氢-乙酸预处理巨龙竹酶解和发酵的影响[J]. 林产化学与工业, 2023, 43(5): 109-116.

Gaoyuan LIU, Zhengjun SHI, Ping ZHAO, Haiyan YANG, Jing YANG. Effects of Sulfuric Acid on Enzymatic Hydrolysis and Fermentation of Hydrogen Peroxide-acetic Acid Pretreated Dendrocalamus sinicus[J]. Chemistry and Industry of Forest Products, 2023, 43(5): 109-116.

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样品 sample	硫酸用量 H₂SO₄ dosage	固体回收率 solid recovery	w_Glu	w_Xyl	w_Lig	木质素脱除率 lignin removal rate
DS		100±0.23	50.53±0.92	19.18±0.22	27.84±0.11	—
DS/PA-0	0	87.82±1.00	56.68±1.33	18.37±0.13	23.36±0.73	28.68±0.23
DS/PA-0.1	0.10	86.56±0.97	59.86±1.13	19.46±1.56	19.09±2.01	38.20±1.00
DS/PA-0.25	0.25	81.54±1.56	63.46±0.97	20.57±1.66	13.12±1.85	56.15±1.56
DS/PA-0.5	0.50	73.08±0.93	68.14±0.56	22.10±1.75	8.73±1.63	80.32±0.93
DS/PA-0.75	0.75	70.85±1.60	70.15±0.72	23.08±0.62	4.60±0.73	86.53±1.60

样品 sample	疏水度/(L·g^-1) hydrophobicity	酶可及度/(mg·g^-1) enzyme accessibility
DS	0.871±0.093	153.12±12.33
DS/PA-0	0.192±0.043	282.15±27.43
DS/PA-0.1	0.128±0.013	450.62±33.14
DS/PA-0.25	0.124±0.014	476.35±31.02
DS/PA-0.5	0.123±0.009	500.12±40.31
DS/PA-0.75	0.168±0.017	497.31±39.75

样品 sample	氧碳比 ratio of oxygen to carbon	表面覆盖率surface coverage		C—C/C—H	C—O	C=O	O—C=O
样品 sample	氧碳比 ratio of oxygen to carbon	S_L	S_C	C—C/C—H	C—O	C=O	O—C=O
DS	52	62	38	46.57	36.66	7.61	9.16
DS/PA-0	69	28	72	31.99	43.37	14.83	9.81
DS/PA-0.5	78	10	90	28.04	51.78	12.58	7.60

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