黑曲霉全细胞生物催化制备没食子酸丙酯的研究

林产化学与工业 ›› 2012, Vol. 32 ›› Issue (6): 52-56.

黑曲霉全细胞生物催化制备没食子酸丙酯的研究

裴建军^1,2, 姚凌菱¹, 李迅^1,2, 赵林果^1,2

1. 南京林业大学化学工程学院, 江苏南京 210037;2. 江苏省生物质绿色燃料与化学品重点实验室, 江苏南京 210037

收稿日期:2012-03-19 修回日期:1900-01-01 出版日期:2013-12-30 发布日期:2013-12-30
通讯作者: 赵林果,博士生导师,主要从事生物质能源及生物催化等领域的研究。

Preparation of Propyl Gallate Catalyzed by the Whole Cells of Aspergillus niger

PEI Jian-jun^1,2, YAO Ling-ling¹, LI Xun^1,2, ZHAO Lin-guo^1,2

1. College of Chemical Engineering,Nanjing Forestry University, Nanjing 210037, China;2. Jiangsu Key Lab of Biomass Based Green Fuels and Chemicals, Naning 210037, China

Received:2012-03-19 Revised:1900-01-01 Online:2013-12-30 Published:2013-12-30

摘要/Abstract

摘要： 利用黑曲霉(Aspergillus niger)细胞作为全细胞生物催化剂,研究了催化没食子酸生成没食子酸丙酯的条件,探讨了有机溶剂、细胞预处理、底物浓度、反应时间和水分含量等因素在酶催化没食子酸丙酯合成中的影响,结果表明,苯是最佳溶剂,而菌丝体的含水量在80%时得率最高。在此基础上,选择菌丝量、没食子酸浓度、正丙醇体积分数和反应时间进行了正交试验,在200r/min、40℃的转化条件下得到的较佳催化组合为:25mL锥形瓶中加入10mL苯、0.5g菌丝、7mmol(0.0127g)没食子酸、7.3%(0.73mL)正丙醇组成的有机催化体系中,反应18h,没食子酸丙酯得率达到36.4%。该过程无需要纯化或者固定化酶,实现了没食子酸丙酯低成本、高得率的催化。

关键词: 没食子酸丙酯, 全细胞催化剂, 有机催化, 黑曲霉

Abstract: In the present study, Aspergillus niger cells were used as a whole-cell biocatalyst for propyl gallate production. The effects of various organic solvents, pre-treatment of the cells, substrate concentration, reaction time, and the water content in the mixture on the enzymatic reactions were investigated. Results showed that benzene was the most suitable solvent and the moisture content of mycelium was 80%. On that basis, the orthogonal experiment was performed. The maximum molar conversion (36.4%) was achieved with 0.5g hyphaes, 7.3% 1-propanol and 7mmol/L gallic acid at stirring speeds of 200r/min and reaction temperature of 40℃ in 25mL conical flask. The process has several advantages, such as mild reaction conditions, less environmental pollution, less accessory substance, and so on.

Key words: propyl gallate, whole-cell biocatalyst, organic catalyst, Aspergillus niger

中图分类号:

TQ35
Q939.9

裴建军;姚凌菱;李迅;赵林果;. 黑曲霉全细胞生物催化制备没食子酸丙酯的研究[J]. 林产化学与工业, 2012, 32(6): 52-56.

PEI Jian-jun;YAO Ling-ling;LI Xun;ZHAO Lin-guo;. Preparation of Propyl Gallate Catalyzed by the Whole Cells of Aspergillus niger[J]. , 2012, 32(6): 52-56.

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