β-葡萄糖苷酶的制备及在纤维素辅助水解上的应用研究

林产化学与工业 ›› 2009, Vol. 29 ›› Issue (1): 13-17.

β-葡萄糖苷酶的制备及在纤维素辅助水解上的应用研究

瞿丽莉, 朱均均, 刘敏, 勇强, 余世袁

南京林业大学林木遗传与生物技术省部共建教育部重点实验室, 江苏南京 210037

收稿日期:2008-02-03 修回日期:1900-01-01 出版日期:2009-02-28 发布日期:2009-02-28
通讯作者: 勇强,教授,博士生导师,主要从事生物质资源生物降解与转化和制浆造纸生物技术的研究。

Study on Preparation of β-Glucosidase and Its Application in Enzymatic Hydrolysis of Cellulose

QU Li-li, ZHU Jun-jun, LIU Min, YONG Qiang, YU Shi-yuan

Key Laboratory of Forest Genetics & Biotechnology, Ministry of Education, Nanjing Forestry University, Nanjing 210037, China

Received:2008-02-03 Revised:1900-01-01 Online:2009-02-28 Published:2009-02-28

摘要/Abstract

摘要： 研究了固体发酵法制备β-葡萄糖苷酶及其在纤维素水解上的应用。黑曲霉NL02以玉米芯和麸皮为碳源固体发酵制备β-葡萄糖苷酶,培养5d,酶活力达到225.43IU/g(以干曲计)。粗β-葡萄糖苷酶酶液经硫酸铵沉淀、离子交换层析、凝胶过滤层析纯化,获得单一β-葡萄糖苷酶组分,酶活回收率和比活力分别为69.34%和133.88IU/mg。底物质量浓度为100g/L的稀硫酸预处理玉米秸秆,经酶用量为20FPIU/g(以纤维素计)的里氏木霉纤维素酶和4IU/g(以纤维素计)的β-葡萄糖苷酶水解48h,水解糖液中纤维二糖和葡萄糖质量浓度分别为1.12和42.68g/L,纤维素水解得率和可发酵性糖的比例分别为62.85%和97.44%。

关键词: 黑曲霉, β-葡萄糖苷酶, 纤维素水解, 纤维二糖

Abstract: Preparation of β-glucosidase by solid-state fermentation and its application in enzymatic hydrolysis of cellulose were investigated. The maximal β-glucosidase activity was 225.43IU/g dry base (based on koji) when Aspergillus niger NL02 was employed using corn cob and wheat bran as carbon sources for 5 days cultivation. β-Glucosidase was purified to homogeneity by ammonium sulfate precipitation, anion-exchange chromatography and gel filtration chromatography. The recovery of enzyme acti-vity and specific activity were 69.34% and 133.88IU/mg, respectively. Corn stalk pretreated by dilute sulfuric acid was hydrolyzed by the dosages of 20FPIU/g (based on cellulose) Trichoderma reesei cellulase and 4IU/g(based on cellulose) β-glucosidase at the substrate concentration of 100g/L for 48h, the concentration of cellobiose and glucose in hydrolysate were 1.12 and 42.68g/L, respectively, the yield of enzymatic hydrolysis and the ratio of fermentable sugar were 62.85% and 97.44%, respectively.

Key words: Aspergillus niger, β-glucosidase, cellulose hydrolysis, cellobiose

中图分类号:

TQ351
Q556.2

瞿丽莉;朱均均;刘敏;勇强;余世袁. β-葡萄糖苷酶的制备及在纤维素辅助水解上的应用研究[J]. 林产化学与工业, 2009, 29(1): 13-17.

QU Li-li;ZHU Jun-jun;LIU Min;YONG Qiang;YU Shi-yuan. Study on Preparation of β-Glucosidase and Its Application in Enzymatic Hydrolysis of Cellulose[J]. , 2009, 29(1): 13-17.

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