大血藤原花色素抗氧化活性及酶抑制活性研究

doi:10.3969/j.issn.0253-2417.2017.03.010

Abstract

Abstract: Proanthocyanidins were extracted from Sargentodoxa cuneata (Oliv) Rehd. et Wils., and the crude extract was purified and isolated. The content of proanthocyanidins was determined by an improved vanillin-HCl colorimetric method. Meanwhile, the antioxidant activity and enzyme inhibitory activity of each sample were measured. The results showed that the contents of proanthocyanidins in the crude extract(CE), petroleum ether fraction(PE), ethyl acetate insoluble fraction(EIF), ethyl acetate fraction(EF), n-butanol fraction(BF) and water residue(WR) were (36.03±0.40)%, (1.19±0.11)%, (57.99±0.22)%, (32.40±0.07)%, (25.03±0.08)% and (41.32±0.37)%, respectively. Moreover, the proanthocyanidin content of the sample purified by Sephadex LH-20 was (98.35±0.72)%, which was 2.73 times of that of the crude extract. The DPPH free radical scavenging activity of the crude extract(IC₅₀=178.10±2.67 mg/L) was 1.12 times of that of L-ascorbic acid(IC₅₀=78.89±1.67 mg/L); the α-glucosidase inhibitory activity of purified sample was 120.71 times of that of acarbose and the corresponding acetylcholine inhibitory activity was 1.14 times of that of tacrine. It showed that there were abundant proanthocyanidins in S. cuneata and the proanthocyanidins had an obvious antioxidant activity and enzyme inhibitory activity in vitro.

Key words: proanthocyanidins, DPPH free radical scavenging activity, glycosidase inhibitory activity, cholinesterase inhibitory activity

CLC Number:

TQ35

LI Yan, LI Xin, LIU Jingling, DENG Kaihang, LIANG Zongsuo. Antioxidant Activity and Enzyme Inhibitory Activity of Proanthocyanidins from Sargentodoxa cuneata(Oliv) Rehd.et Wils.[J]. Chemistry and Industry of Forest Products, 2017, 37(3): 73-81.

References

[1] 国家药典委员会.中华人民共和国药典:一部[M].2015年版.北京:中国医药科技出版社,2015:194. Chinese Pharmacopoeia Commission. Pharmacopoeia of China:1 st section[M].Beijing:China Medical Science and Technology Press, 2015:194.
[2] WANG Q H, KUANG H X, SU Y, et al. Naturally derived anti-inflammatory compounds from Chinese medicinal plants[J]. Journal of Ethnopharmacology, 2013, 146(1):9-39.
[3] KRISHNAIAH D, SARBATLY R, NITHYANANDAM R. A review of the antioxidant potential of medicinal plant species[J]. Food and Bioproducts Processing, 2011, 89(3):217-233.
[4] ZENG X B, WANG H, GONG Z Q, et al. Antimicrobial and cytotoxic phenolics and phenolic glycosides from Sargentodoxa cuneata[J]. Fitoterapia, 2015, 101:153-161.
[5] SHAYGANNI E, BAHMANI M, ASGARY S, et al. Inflammaging and cardiovascular disease:Management by medicinal plants[J]. Phytomedicine, 2016, 23(1):1119-1126.
[6] 陈丽珍,周英,黄俊飞,等.大血藤对破骨细胞活性及成骨细胞增殖分化作用的研究[J].中国中药杂志,2015,40(22):4463-4468. CHEN L Z, ZHOU Y, HUANG J F,et al. Research on effect of Sargentodoxae caulis on activity of osteoclasts and proliferation differentiation of osteoblasts.[J].China Journal of Chinese Materia Medica, 2015, 40(22):4463-4468.
[7] 孙达旺.植物单宁化学[M].北京:中国林业出版社,1992. SUN D W. Plant Tannins Chemical[M].Beijing:China Forestry Publishing House,1992.
[8] SCHAFER A, HOGGER P. Oligomeric procyanidins of French maritime pine bark extract (Pycnogenol^®) effectively inhibit α-glucosidase[J].Diabetes Research and Clinical Practice, 2007, 77(1):41-46.
[9] LAVELLI V, SRI HARSHA P S C, FERRANTI P, et al. Grape skin phenolics as inhibitors of mammalian α-glucosidase and α-amylase-effect of food matrix and processing on efficacy[J]. Food & Function, 2016, 7(3):1655-1663.
[10] FU C L, YANG X N, LAI S J, et al. Structure, antioxidant and α-amylase inhibitory activities of longan pericarp proanthocyanidins[J]. Journal of Functional Foods, 2015, 14:23-32.
[11] WANG H Y, LIU T T, SONG L X, et al. Profiles and α-amylase inhibition activity of proanthocyanidins in unripe Manilkara zapota (chiku)[J]. Journal of Agricultural and Food Chemistry, 2012, 60(12):3098-3104.
[12] GONÇALVES R, MATEUS N, DE FREITAS V. Inhibition of α-amylase activity by condensed tannins[J]. Food Chemistry, 2011, 125(2):665-672.
[13] CHOI J S, BHAKTA H K, FUJⅡ H, et al. Inhibitory evaluation of oligonol on α-glucosidase, protein tyrosine phosphatase 1B, cholinesterase, and β-secretase 1 related to diabetes and Alzheimer's disease[J]. Archives of pharmacal research, 2016, 39(3):409-420.
[14] WANG Y J, THOMAS P, ZHONG J H, et al. Consumption of grape seed extract prevents amyloid-β deposition and attenuates inflammation in brain of an Alzheimer's disease mouse[J]. Neurotoxicity Research, 2009, 15(1):3-14.
[15] WANG J, FERRUZZI M G, HO L, et al. Brain-targeted proanthocyanidin metabolites for Alzheimer's disease treatment[J]. Journal of Neuroscience, 2012, 32(15):5144-5150.
[16] FERRUZZI M G, LOBO J K, JANLE E M, et al. Bioavailability of gallic acid and catechins from grape seed polyphenol extract is improved by repeated dosing in rats:Implications for treatment in Alzheimer's disease[J]. Journal of Alzheimers Disease, 2009, 18(1):113-124.
[17] 袁贤达.大血藤化学成分研究[D].北京:中国中医科学院中药研究所硕士学位论文,2013. YUAN X D. Study on the chemical ingredients in Sargentodoxa cuneata[D].Beijing:Master Degree Thesis of Chinese Materia Medica China Academy of Chinese Medical Science, 2013.
[18] 陈智仙,高文远,刘岱琳,等.大血藤的化学成分研究[J].中草药,2010,41(6):867-870. CHEN Z X, GAO W Y, LIU D L,et al. Study on the chemical ingredients in Sargentodoxa cuneata[J].Chinese Traditional and Herbal Drugs,2010,41(6):867-870.
[19] 田瑛,张慧娟,屠爱萍,等.中药大血藤的酚性化合物[J].药学学报,2005,40(7):628-631. TIAN Y, ZHANG H J, TU A P, et al.Phenolics from traditional Chinese medicine Sargentodoxa cuneata[J].Acta Pharmaceutica Sinica, 2005,40(7):628-631.
[20] 毛水春.中药大血藤Sargentodoxa cuneata抗癌活性成分的分离与鉴定:化合物的结构研究[D].青岛:中国海洋大学博士学位论文,2003. MAO S C. Isolation and identification of the anticancer active constituents of Sargentodoxa cuneata,A Chinese herb[D].Qingdao:Doctoral Dissertation of Ocean University of China,2003.
[21] 李钧敏,金则新,陈彤,等.红藤饮片提取物抑菌活性与次生代谢产物含量的相关性[J].浙江大学报:医学版,2006,35(3):273-280. LI J M, JIN Z X, CHEN T, et al. Correlation of anti-bacterial activity with secondary metabolites content in Sargentodoxa Cuneata tables[J].Journal of Zhe Jiang University:Medical Sciences,2006,35(3):273-280.
[22] CHAI W M, SHI Y, FENG H L, et al. Structure characterization and anti-tyrosinase mechanism of polymeric proanthocyanidins fractionated from kiwifruit pericarp[J]. Journal of Agricultural and Food Chemistry, 2014, 62(27):6382-6389.
[23] SUN B, RICARDO-DA-SILVA J M, SPRANGER I. Critical factors of vanillin assay for catechins and proanthocyanidins[J] Journal of Agricultural and Food Chemistry, 1998, 46(10):4267-4274.
[24] SHARMA O P, BHAT T K. DPPH antioxidant assay revisited[J]. Food Chemistry, 2009, 113(4):1202-1205.
[25] BENZIE I F F, STRAIN J J. The ferric reducing ability of plasma (FRAP) as a measure of ‘antioxidant power’:The FRAP assay[J]. Analytical Biochemistry, 1996, 239(1):70-76.
[26] YAMAGUCHI M, WAKASUGI J, SAKAKIMA J. Competitive and product inhibition-based α-amylase activity analysis method[J]. Clinical Biochemistry, 2008, 41(4/5):325-330.
[27] DONG H Q, LI M, ZHU F, et al. Inhibitory potential of trilobatin from lithocarpus polystachyus rehd against α-glucosidase and α-amylase linked to type 2 diabetes[J]. Food Chemistry, 2012, 130(2):261-266.
[28] ELLMAN G L, COURTNEY K D, JR ANDRES V, et al. A new and rapid colorimetric determination of acetylcholinesterase activity[J]. Biochemical Pharmacology, 1961, 7(2):88-95.
[29] 程悦,符影,王志宇,等.鸡血藤提取物中缩合鞣质的含量测定及其抗肿瘤活性初步研究[J].中山大学学报:自然科学版, 2011,50(2):75-80. CHENG Y, FU Y, WANG Z Y, et al. Determination on the contents of condensed tannins in Spatholobus suberectus Dunn. extracts and primary study on their anti-tumor activities[J]. Acta Scientiarum Naturalium Universitatis Sunyatseni, 2011,50(2):75-80.

Antioxidant Activity and Enzyme Inhibitory Activity of Proanthocyanidins from Sargentodoxa cuneata(Oliv) Rehd.et Wils.

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	ZHOU Meng, LIU Gongjun, HE Lingxiao, WANG Fei. Preparation and Antioxidant Activity of Proanthocyanidins Dimers from Acacia mearnsii De Willd [J]. Chemistry and Industry of Forest Products, 2017, 37(2): 135-140.
[2]	JIANG Gui-quan, FANG Gui-zhen, SHI Zhen-xin, ZHANG Zhuo-rui, PANG Jiu-yin. Cellulose Enzymatic-assisted Extraction of Proanthocyanidins from the Larch Bark after Degreasing by Supercritical CO₂ [J]. Chemistry and Industry of Forest Products, 2014, 34(2): 97-102.
[3]	ZHAO Zhi-jie;XIONG Jia;WANG Fei. Purification, Degradation and Scavenging Activities on DPPH Free Radical of Proanthocyanidins from Black Wattle Bark [J]. , 2013, 33(4): 83-88.
[4]	JIANG Yong-xin;ZHU Hong-tao;WANG Jun-min;ZHANG Ying-jun;ZHANG Jia-yan;HUA Yan;ZHAO Ping. LC-ESI-MS Analysis of Fragmentive Products of Pine Bark Proanthocyanidins [J]. , 2013, 33(4): 117-120.
[5]	LI Tian-tian;WANG Fei. Investigation on Ultrasonic-assisted Extraction of Proanthocyanidins from Black Wattle Bark [J]. , 2012, 32(5): 56-62.
[6]	XU Man;WANG Yong-mei;ZHANG Liang-liang;WU Dong-mei;CHEN Jia-hong. Comparison of the Antioxidant Activity of the Bark Extracts of Myrica esculenta,Phyllanthus emblica and Larix gmelinii [J]. , 2011, 31(6): 41-45.
[7]	WU Dong-mei;CHEN Jia-hong;WANG Yong-mei;XU Man;WU Zai-song. Study on Ultrasound-assisted Extraction of Proanthocyanidins from Myrica esculenta Bark [J]. , 2009, 29(S1): 105-109.
[8]	XU Man;CHEN Jia-hong;WANG Yong-mei;WU Dong-mei;WU Zai-song. Preliminary Report of Extraction and Analysis of Tannins from Camellia oleifera Fruit Shell [J]. , 2009, 29(S1): 187-191.
[9]	WANG Yong-mei;CHEN Jia-hong;WU Dong-mei;XU Man;WU Zai-song. Study on Microwave-assisted Extraction of Proanthocyanidins from Myrica esculenta Bark [J]. , 2009, 29(S1): 195-198.
[10]	JIA Jia;YANG Lei;ZU Yuan-gang. Homogenated Extraction of Oligomeric Proanthocyanidins from Larch Bark and Its Optimization by Response Surface Methodology [J]. , 2009, 29(3): 78-84.
[11]	WANG Yong-mei;CHEN Jia-hong;WU Dong-mei;XU Man;WU Zai-song. Primary Study on Antioxidative and Radical Scavenging Activities of Extracts from Phyllanthus emblica L. Bark [J]. , 2008, 28(1): 11-15.
[12]	CHEN Jia-hong;WANG Yong-mei;WU Dong-mei;WU Zai-song. Preliminary Study on Antioxidative and Radical-scavenging Activities of Extracts from Myrica esculenta Buch. -Ham. Bark [J]. , 2007, 27(S1): 1-7.
[13]	LIU Xia-rui;WANG Fei. Investigation on Biological Activities of Proanthocyanidins from Black Wattle Bark [J]. , 2007, 27(3): 43-48.
[14]	LUO Xin;YAO Kai;JIA Dong-ying;HE Qiang. Proanthocyanidins: Absorption, Metabolism and Bioavailability [J]. , 2006, 26(1): 109-115.
[15]	YANG Lei;SU Wen-qiang;WANG Zhen-yang;YANG Dong-mei. STUDY ON DETERMINATION METHOD OF OLIGOMERIC PROANTHOCYANIDINS CONTENT IN EXTRACTS OF LARCH BARK [J]. , 2004, 24(S1): 111-114.