花椒叶-金露梅叶复合饮料的制备及其抗氧化活性研究

doi:10.3969/j.issn.0253-2417.2022.02.016

Abstract

Abstract:

The Zanthoxylum bungeanum leaves extracts(ZBE) and Potentilla fruticosa leaves extracts(PFE) were prepared by ultrasonic assisted extraction and macroporous adsorptive resins, and the optimal mixing ratio was determined by isoradiation analysis. The results showed that the mixture of ZBE and PFE(mass ratio 3:1) exhibited the strongest synergistic antioxidant interaction. The formulation of ZBE and PFE compound beverage was as follows: the mixture of ZBE and PFE(3:1) 0.10%, xylitol 0.015%, aspartame 8.00%, citric acid 0.10%, CMC-Na 0.30%, xanthan gum 0.02%, and β-cyclodextrin 0.40%, 0.05% potassium sorbate. After boiling sterilization for 30 min, the total colonies numbers, mold and yeast numbers, coliform numbers, and foodborne pathogenic numbers of beverage were all meet the national standards, the total phenol content(TPC) was 0.014 1 μmol/g, the total flavonoid content(TFC) was 0.019 8 μmol/g, the content of the six main components catechin, ferulic acid, hypericin, rutin, quercitrin and afzelin were(28.76±0.20), (13.72±0.16), (65.32±0.55), (19.85±0.27), (62.76±0.36), (16.78±0.22) mg/g, the ABTS⁺· free radical scavenging capability was (2.09±0.011) μmol/g, the FRAP trivalent iron reduction capacity was (1.21±0.003) μmol/g, the mass concentration of the samples required to removing 50% DPPH·(IC₅₀) was (436.48±3.16) mg/L, it exhibited strong antioxidant effects. Furthermore, after chemical stability test and the prediction and verification tests of shelf life, the shelf life at (25±2) ℃ was 6 months.

Key words: Zanthoxylum bungeanum leaves, Potentilla fruticosaleaves, compound beverage, synergistic antioxidant interaction

CLC Number:

TQ35

Ruofei ZHENG, Zijia LIU, Nuernisa KASIMU, Dongmei WANG. Preparation and Antioxidant Effect of Zanthoxylum bungeanum Leaves and Potentilla fruticosa Leaves Extracts Compound Beverage[J]. Chemistry and Industry of Forest Products, 2022, 42(2): 115-124.

Figures/Tables 9

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References 34

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m_ZBE: m_PFE	IC₅₀/(mg·L^-1)		γ
m_ZBE: m_PFE	理论值 expected	测定值 observed	γ
1:0		7.13±0.35
0:1		1.42±0.24
2:1	3.01±0.74	2.30±0.13	0.73
3:1	3.52±0.82	2.39±0.17	0.68
4:1	3.91±0.99	2.68±0.78	0.69
5:1	4.23±0.45	2.92±0.24	0.69

m_ZBE: m_PFE	TPC/(μmol·g^-1)	TFC/(μmol·g^-1)	CAT/(mg·g^-1)	FER/(mg·g^-1)	HYD/(mg·g^-1)	RUT/(mg·g^-1)	QUE/(mg·g^-1)	AFZ/(mg·g^-1)
1:0	1.9231±0.0087	2.2596±0.0359	—	—	124.39±3.93	38.57±1.31	125.74±5.52	33.76±0.97
0:1	1.6135±0.0095	2.3309±0.0201	110.97±2.44	36.09±1.77	—	—	—	—
2:1	1.7805±0.0087	2.3070±0.0109	42.53±2.63	17.24±0.49	73.42±2.00	22.46±1.31	71.12±1.85	15.38±0.44
3:1	1.8432±0.0144	2.2881±0.0145	36.23±1.91	15.34±0.34	75.21±1.30	25.43±0.51	75.77±2.25	21.52±0.49
4:1	1.8489±0.0066	2.2691±0.0055	24.87±1.44	8.75±0.21	86.75±2.15	31.56±0.75	85.29±2.17	25.33±0.95
5:1	1.8946±0.0087	2.2596±0.0095	17.41±0.62	6.75±0.07	95.32±2.13	33.89±1.47	94.27±2.24	28.47±1.61

添加量/% addition	TPC/(μmol·g^-1)	TFC/(μmol·g^-1)	R_ABTS⁺·/(μmol·g^-1)	R_FRAP/(μmol·g^-1)	IC_{50, DPPH·}/(mg·L^-1)
0.05	0.0102	0.0153	1.74±0.009	0.85±0.010	3.54±0.05
0.10	0.0157	0.0215	2.37±0.011	1.37±0.008	3.22±0.07
0.15	0.0187	0.0258	2.91±0.012	1.76±0.007	3.15±0.08
0.20	0.0223	0.0302	3.36±0.015	2.11±0.008	3.28±0.08

试验号 No.	A	B	C	沉降率/% sedimentation rate	试验号 No.	A	B	C	得分 score
试验号 No.	羧甲基纤维素钠/% CMC-Na	黄原胶/% xanthan gum	β-环状糊精/% β-cyclodextrin	沉降率/% sedimentation rate	试验号 No.	阿斯巴甜/% aspartame	木糖醇/% xylitol	柠檬酸/% citric acid	得分 score
1	0.20	0.02	0.40	1.08	1	6.00	0.005	0.05	75.07
2	0.20	0.03	0.50	2.76	2	6.00	0.010	0.10	78.62
3	0.20	0.04	0.60	2.58	3	6.00	0.015	0.15	78.19
4	0.25	0.02	0.60	2.16	4	7.00	0.005	0.15	77.92
5	0.25	0.03	0.40	2.01	5	7.00	0.010	0.05	74.86
6	0.25	0.04	0.50	2.52	6	7.00	0.015	0.10	76.98
7	0.30	0.02	0.50	0.92	7	8.00	0.005	0.10	78.69
8	0.30	0.03	0.60	1.80	8	8.00	0.010	0.15	77.65
9	0.30	0.04	0.40	2.62	9	8.00	0.015	0.05	80.43
k₁	2.14	1.39	1.90		k₁	77.29	77.23	76.79
k₂	2.23	2.19	2.07		k₂	76.59	77.04	78.10
k₃	1.78	2.57	2.18		k₃	78.92	78.53	77.92
R	0.45	1.18	0.28		R	2.33	1.49	1.31

灭菌工艺 treatment		菌落总数/(CFU·mL^-1) aerobic plate count	霉菌和酵母菌/(CFU·mL^-1) mould & yeast	大肠菌群/(MPN·mL^-1) coliform	食源性致病菌 foodborne pathogen
GB 16740		≤100	≤20	≤0.1	阴性negative(ND)
未灭菌non-sterilized		4200	600	28	阴性negative(ND)
微波灭菌 microwave sterilization	30 s	2900	500	16	阴性negative(ND)
	60 s	1500	300	15	阴性negative(ND)
	90 s	600	200	14	阴性negative(ND)
煮沸灭菌 boiling sterilization	15 min	20	60	阴性negative(ND)	阴性negative(ND)
	20 min	＜10	30	阴性negative(ND)	阴性negative(ND)
	25 min	＜10	10	阴性negative(ND)	阴性negative(ND)
	30 min	ND	ND	阴性negative(ND)	阴性negative(ND)
高压蒸汽灭菌 high pressure steam sterilization	121 ℃，15 min	＜10	ND	阴性negative(ND)	阴性negative(ND)

Preparation and Antioxidant Effect of Zanthoxylum bungeanum Leaves and Potentilla fruticosa Leaves Extracts Compound Beverage

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化学稳定性试验 chemical stability test	天数/d day	TPC/(μmol·g^-1)	TFC/(μmol·g^-1)	R_ABTS⁺·/(μmol·g^-1)	R_FRAP/(μmol·g^-1)	CAT/(mg·g^-1)	FER/(mg·g^-1)	HYP/(mg·g^-1)	RUT/(mg·g^-1)	QUE/(mg·g^-1)	AFZ/(mg·g^-1)
高温试验 high temperature assays	0	0.0141	0.0198	2.09±0.01	1.21± 0.03	28.76±0.20	13.72± 0.16	65.32±0.55	19.85± 0.27	62.76±0.36	16.78± 0.22
	5	0.0137	0.0194	2.04±0.02	1.19± 0.02	27.96±0.51	13.52± 0.44	63.89±0.55	19.12± 0.62	61.52±0.39	16.22± 0.33
	10	0.0134	0.019	2.00±0.02	1.18± 0.03	27.48±0.42	13.35± 0.52	62.24±0.35	19.02± 0.47	60.78±0.26	16.08± 0.35
强光试验 strong illu mination assays	5	0.0138	0.0196	2.05±0.04	1.19± 0.05	28.52±0.54	13.48± 0.27	64.3±0.37	19.58± 0.25	62.05±0.61	16.34± 0.24
强光试验 strong illu mination assays	10	0.0136	0.019	2.02±0.04	1.17± 0.05	27.89±0.43	13.22± 0.52	62.31±0.36	19.23± 0.24	61.57±0.24	16.16± 0.12
加速试验 accelerated tests	30	0.014	0.0196	2.07±0.07	1.20± 0.04	28.40±0.57	13.64± 0.52	63.69±0.44	19.83± 0.34	62.18±0.31	16.68± 0.33
	60	0.0138	0.0194	2.03±0.02	1.18± 0.02	28.38±0.53	13.50± 0.27	63.76±0.34	19.47± 0.25	62.16±0.24	16.39± 0.32
	90	0.0137	0.0194	2.02±0.05	1.17± 0.06	27.19±0.45	13.43± 0.47	62.86±0.34	19.31± 0.31	61.07±0.41	16.35± 0.30
	180	0.0135	0.019	1.99±0.04	1.16± 0.02	27.38±0.33	13.15± 0.37	62.90±0.34	19.02± 0.47	60.86±0.24	16.11± 0.29

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灭菌工艺 treatment	R_ABTS⁺·/(μmol·g^-1)	R_FRAP/(μmol·g^-1)	IC_{50, DPPH·}/(mg·L^-1)	TPC/(μmol·g^-1)	TFC/(μmol·g^-1)	CAT/(mg·g^-1)	FER/(mg·g^-1)	HYP/(mg·g^-1)	RUT/(mg·g^-1)	QUE/(mg·g^-1)	AFZ/(mg·g^-1)
未灭菌 non-sterilization	2.17±0.032	1.26± 0.006	343.94±1.23	0.0148	0.0203	30.03± 0.78	14.12±0.47	68.42± 0.56	20.31±0.39	63.21± 0.52	17.21±0.34
高压蒸汽灭菌 high pressure steam sterilization	2.06±0.028	1.2± 0.008	477.88±2.24	0.0138	0.019	28.21± 0.66	13.21±0.37	67.22± 0.54	19.57±0.52	61.21± 0.46	16.73±0.35
煮沸灭菌 boiling sterilization	2.09±0.031	1.21± 0.005	436.48±1.35	0.0141	0.0198	28.76± 0.71	13.72±0.51	65.32± 0.64	19.85±0.48	62.76± 0.42	16.78±0.28