高浓度木质纤维素原料酶解过程分析

doi:10.3969/j.issn.0253-2417.2022.04.009

Abstract

Abstract:

Focusing on alkali-treated bagasse, the effects of solid-liquid mixing methods, addition of three kinds of deep eutectic solvents(DES), feed method of fed-batch feeding, and the addition of xylanase on the enzymatic hydrolysis efficiency at high solid loading were investigated. The results showed that magnetic stirring could accelerate enzymatic hydrolysis speed, but had little effect on the efficiency of enzymatic hydrolysis. Negligible effect on enzymatic hydrolysis was observed by addition of low-concentration DES. A fed-batch with initial substrate loading of 150 g/L was fed at 12 and 36 h, respectively, which resulted in a total solid loading of 350 g/L for enzymatic hydrolysis. After enzymatic hydrolysis for 96 h, the total sugar concentration reached 162.7 g/L with the conversion rates of approximately 51% of both cellulose and hemicellulose. On the basis of fed-batch feeding, 1 000 IU/g hemicellulase was added, and the enzyme cocktail was used for enzymatic hydrolysis for 96 h. The total sugar concentration reached 218.9 g/L, and the conversion rate of cellulose and hemicellulose were 72% and 63%, respectively, which were 40% and 22% higher than those without the addition of hemicellulase.

Key words: lignocellulose, high solid loading, enzymatic hydrolysis, fed-batch, hemicellulase

CLC Number:

TQ35
TK6

Xiaoyan CHEN, Rongqing ZHANG, Yating JIAN, Qiang YU, Zhongming WANG, Zhenhong YUAN. Analysis of Enzymatic Hydrolysis Process of High-solid Lignocellulose Materials[J]. Chemistry and Industry of Forest Products, 2022, 42(4): 61-67.

Figures/Tables 5

Table 1

Fig.1

Fig.2

Table 2

Fig.3

References 27

1	DI R S , HU C S , SAVILLE B A , et al. Large-scale, high-solids enzymatic hydrolysis of steam-exploded poplar[J]. Biofuels, Bioproducts and Biorefining, 2011, 5 (6): 609- 620. doi: 10.1002/bbb.323
2	HODGE D B , KARIM M N , SCHELL D J , et al. Model-based fed-batch for high-solids enzymatic cellulose hydrolysis[J]. Applied Biochemistry and Biotechnology, 2009, 152 (1): 88- 107. doi: 10.1007/s12010-008-8217-0
3	WANG W , KANG L , WEI H , et al. Study on the decreased sugar yield in enzymatic hydrolysis of cellulosic substrate at high solid loading[J]. Applied Biochemistry and Biotechnology, 2011, 164 (7): 1139- 1149. doi: 10.1007/s12010-011-9200-8
4	KADIĆ A , PALMQVIST B , LIDÉN G . Effects of agitation on particle-size distribution and enzymatic hydrolysis of pretreated spruce and giant reed[J]. Biotechnology for Biofuels, 2014, 7:77- 87. doi: 10.1186/1754-6834-7-77
5	KADIC' A , LIDÉN G . Does sugar inhibition explain mixing effects in enzymatic hydrolysis of lignocellulose?[J]. Journal of Chemical Technology & Biotechnology, 2017, 92 (4): 868- 873.
6	刘云云, 张宇, 冯胜, 等. 甜高粱渣分批补料酶水解生产高浓度糖[J]. 太阳能学报, 2015, 36 (3): 671- 676. doi: 10.3969/j.issn.0254-0096.2015.03.025
	LIU Y Y , ZHANG Y , FENG S , et al. High concentration sugar production from sweet sorghum bagasse using fed-batch hydrolysis[J]. Acta Energiae Solaris Sinica, 2015, 36 (3): 671- 676. doi: 10.3969/j.issn.0254-0096.2015.03.025
7	DA SILVA A S , ESPINHEIRA R P , TEIXEIRA R S S , et al. Constraints and advances in high-solids enzymatic hydrolysis of lignocellulosic biomass: A critical review[J]. Biotechnology for Biofuels, 2020, 13:58- 86. doi: 10.1186/s13068-020-01697-w
8	LIU Z H , CHEN H Z . Periodic peristalsis releasing constrained water in high solids enzymatic hydrolysis of steam exploded corn stover[J]. Bioresource Technology, 2016, 205:142- 152. doi: 10.1016/j.biortech.2016.01.037
9	XU C , ZHANG J , ZHANG Y , et al. Enhancement of high-solids enzymatic hydrolysis efficiency of alkali pretreated sugarcane bagasse at low cellulase dosage by fed-batch strategy based on optimized accessory enzymes and additives[J]. Bioresource Technology, 2019, 292:121993- 122000. doi: 10.1016/j.biortech.2019.121993
10	LAU M W , GUNAWAN C , BALAN V , et al. Comparing the fermentation performance of Escherichia coli KO11, Saccharomyces cerevisiae 424A(LNH-ST) and Zymomonas mobilis AX101 for cellulosic ethanol production[J]. Biotechnology for Biofuels, 2010, 3 (1): 11- 21. doi: 10.1186/1754-6834-3-11
11	XUE Y , JAMEEL H , PHILLIPS R , et al. Split addition of enzymes in enzymatic hydrolysis at high solids concentration to increase sugar concentration for bioethanol production[J]. Journal of Industrial and Engineering Chemistry, 2012, 18 (2): 707- 714. doi: 10.1016/j.jiec.2011.11.132
12	ADNEY B, BAKER J. Measurement of cellulase activities: Laboratory Analytical Procedure(LAP): NREL/TP-510-42628[G]. Golden: National Renewable Energy Laboratory, 1996-08-12.
13	WANG W , WANG X , ZHANG Y , et al. Effect of sodium hydroxide pretreatment on physicochemical changes and enzymatic hydrolysis of herbaceous and woody lignocelluloses[J]. Industrial Crops and Products, 2020, 145:112145- 112153. doi: 10.1016/j.indcrop.2020.112145
14	SLUITER A, HAMES B, RUIZ R, et al. Determination of structural carbohydrates and lignin in biomass: Laboratory Analytical Procedure(LAP): NREL/TP-510-42618[G]. Golden: National Renewable Energy Laboratory, 2012-08-03.
15	ABBOTT A P , CAPPER G , DAVIES D L , et al. Novel solvent properties of choline chloride/urea mixtures[J]. Chem Commun(Camb), 2003, (1): 70- 71.
16	KUMAR A K , PARIKH B S , PRAVAKAR M . Natural deep eutectic solvent mediated pretreatment of rice straw: Bioanalytical characterization of lignin extract and enzymatic hydrolysis of pretreated biomass residue[J]. Environmental Science and Pollution Research, 2016, 23 (10): 9265- 9275. doi: 10.1007/s11356-015-4780-4
17	GUNNY A A N , ARBAIN D , NASHEF E M , et al. Applicability evaluation of deep eutectic solvents-cellulase system for lignocellulose hydrolysis[J]. Bioresource Technology, 2015, 181:297- 302. doi: 10.1016/j.biortech.2015.01.057
18	NI Y , BI Z , SU H , et al. Deep eutectic solvent(DES) as both solvent and catalyst for oxidation of furfural to maleic acid and fumaric acid[J]. Green Chemistry, 2019, 21 (5): 1075- 1079. doi: 10.1039/C8GC04022B
19	黄娟. 纤维素生物转化螺带型搅拌反应器工程研究[D]. 上海: 华东理工大学, 2013.
	HUANG J. Lignocellulose bioconversion: Helical ribbon stirred reactor engineering research[D]. Shanghai: East China University of Science and Technology, 2013.
20	PALMQVIST B , WIMAN M , LIDÉN G . Effect of mixing on enzymatic hydrolysis of steam-pretreated spruce: A quantitative analysis of conversion and power consumption[J]. Biotechnology for Biofuels, 2011, 4 (1): 10- 18. doi: 10.1186/1754-6834-4-10
21	TENGBORG C , GALBE M , ZACCHI G . Influence of enzyme loading and physical parameters on the enzymatic hydrolysis of steam-pretreated softwood[J]. Biotechnology Progress, 2001, 17 (1): 110- 117. doi: 10.1021/bp000145+
22	YANG M , LI W , LIU B , et al. High-concentration sugars production from corn stover based on combined pretreatments and fed-batch process[J]. Bioresource Technology, 2010, 101 (13): 4884- 4888. doi: 10.1016/j.biortech.2009.12.013
23	ZHANG Y , LIU Y , XU J , et al. High solid and low enzyme loading based saccharification of agricultural biomass[J]. BioResources, 2012, 7 (1): 345- 353.
24	GAO Y , XU J , YUAN Z , et al. Optimization of fed-batch enzymatic hydrolysis from alkali-pretreated sugarcane bagasse for high-concentration sugar production[J]. Bioresource Technology, 2014, 167:41- 45. doi: 10.1016/j.biortech.2014.05.034
25	WANG W , ZHUANG X , YUAN Z , et al. High consistency enzymatic saccharification of sweet sorghum bagasse pretreated with liquid hot water[J]. Bioresource Technology, 2012, 108:252- 257. doi: 10.1016/j.biortech.2011.12.092
26	ZHAO X , DONG L , CHEN L , et al. Batch and multi-step fed-batch enzymatic saccharification of Formiline-pretreated sugarcane bagasse at high solid loadings for high sugar and ethanol titers[J]. Bioresource Technology, 2013, 135:350- 356. doi: 10.1016/j.biortech.2012.09.074
27	KUMAR R , WYMAN C E . Strong cellulase inhibition by Mannan polysaccharides in cellulose conversion to sugars[J]. Biotechnology and Bioengineering, 2014, 111 (7): 1341- 1353. doi: 10.1002/bit.25218

溶剂solvent	添加量/% addition	糖质量浓度mass concn. of carbohydrate/(g·L^-1)				酶解率enzymolysis rate/%
溶剂solvent	添加量/% addition	纤维二糖cellobiose	葡萄糖glucose	木糖xylose	阿拉伯糖arabinose	纤维素cellulose	半纤维素xylan
对照control	0	13.0	43.8	17.9	1.6	50.4	39.1
ChCl/EG	1	12.2	42.0	16.0	3.7	48.1	39.5
ChCl/G	1	12.5	42.4	16.7	1.5	48.8	36.6
ChCl/U	1	12.6	40.3	15.6	1.3	47.0	33.9
ChCl/EG	5	15.5	36.7	16.3	1.3	46.0	35.1
ChCl/G	5	11.2	35.8	12.6	1.5	41.7	28.3
ChCl/U	5	11.6	31.5	11.3	0.9	38.3	24.6

底物 substrate	初始质量浓度/(g·L^-1) initial concn.	补料方式/(g·L^-1) feeding methods			加酶量/(FPU·g^-1) enzyme dose	酶解时间/h enzymatic time	质量浓度/(g·L^-1) mass concn.		纤维素酶解率/% cellulose enzymolysis rate	文献 ref.
底物 substrate	初始质量浓度/(g·L^-1) initial concn.	1st	2nd	3th	加酶量/(FPU·g^-1) enzyme dose	酶解时间/h enzymatic time	葡萄糖 glucose	木糖 xylose	纤维素酶解率/% cellulose enzymolysis rate	文献 ref.
蔗渣 sugarcane bagasse	150	100 (12 h)	100 (36 h)		20	96	96.5	46.0	51.5	本研究 this study
蔗渣 sugarcane bagasse	150	80 (11 h)	70 (20 h)	60 (28 h)	8.5	96	134.9	60.3	59.8	[6]
蔗渣 sugarcane bagasse	120	70 (6 h)	70 (12 h)	70 (24 h)	10	120	129.5	56.0	59.9	[24]
玉米秆 corn stalk	120	60 (12 h)	60 (36 h)	60 (60 h)	20	144	175.0	20.0	60.0	[22]
麦秆 wheat straw	90	80 (8 h)	70 (24 h)	60 (48 h)	9.6	144	81.9	20.3	34.8	[23]
蔗渣 sugarcane bagasse	150	50 (24 h)	100 (48 h)		10	120	88.9	9.8	59.0	[25]
蔗渣 sugarcane bagasse	70	60 (12 h)	60 (36 h)		10	144	150.0		80.0	[25]

[1]	Guangliu XU, Zhichen ZHAO, Rui ZHANG, Han ZHANG, Junjun ZHU. Fractionation of Bamboo Residues by Acid Hydrotrope [J]. Chemistry and Industry of Forest Products, 2022, 42(4): 16-24.
[2]	Xuchen JIN, Zhouyang XIANG. The Effects of Molecule Weight on the Crystallization Ability of Xylan [J]. Chemistry and Industry of Forest Products, 2022, 42(4): 40-46.
[3]	MA Tengfei, ZHAN Yawei, LIU Yue, LI Zhiqiang. Formaldehyde/Dioxane Pretreatment on Bamboo for Producing Monosaccharide and Lactic Acid [J]. Chemistry and Industry of Forest Products, 2022, 42(4): 101-106.
[4]	Jinyuan CHENG, Yunni ZHAN, Chen HUANG, Yongjun DENG, Guigan FANG. AlCl₃Incorporation Facilitated the Deep Eutectic Solvent Pretreatment [J]. Chemistry and Industry of Forest Products, 2022, 42(2): 56-62.
[5]	Shujie WANG, Xianfeng HOU, Yingshi HUANG, Xiaofeng CHEN, Jin SUN, Zhenzhong GAO. Effect of Alkaline Ionic Liquid TBAH Pretreatment on Structure and Enzymatic Properties of Eucalyptus [J]. Chemistry and Industry of Forest Products, 2022, 42(1): 57-63.
[6]	Xinglong HOU, Xiaopeng JIAN, Wei XU, Qi GUO, Junli LIU. Pyrolysis Behavior Analysis of Enzymolysis Lignin Based on TG-FTIR-MS Technology [J]. Chemistry and Industry of Forest Products, 2021, 41(6): 36-42.
[7]	Peiyao WEN, Jiaxin YOU, Yong XU, Junhua ZHANG. Co-production of Xylooligosaccharides and Bacillus subtilis from Poplar [J]. Chemistry and Industry of Forest Products, 2021, 41(5): 1-7.
[8]	Xin FANG, Peiyao WEN, Yong XU, Junhua ZHANG. Production of Xylooligosaccharides and Monosaccharides from Poplar by Two-step Sodium Chlorite and Sodium Hydroxide Pretreatment [J]. Chemistry and Industry of Forest Products, 2021, 41(5): 23-30.
[9]	Yunni ZHAN, Chen HUANG, Xin HAO, Zimeng WANG, Yongjun DENG, Guigan FANG. Preparation of Xylooligosaccharides and Monosaccharides from Bamboo Chips by Liquid Hot Water Pretreatment [J]. Chemistry and Industry of Forest Products, 2021, 41(1): 77-84.
[10]	Xuan ZHOU, Can JIN, Guifeng LIU, Shuping HUO, Guomin WU, Zhenwu KONG. Progress on Lignocellulose-based Magnetic Adsorbents for Removal of Heavy Metal Ions [J]. Chemistry and Industry of Forest Products, 2021, 41(1): 123-132.
[11]	Jingcong XIE,Yueshu GAO,Jianchun JIANG,Hao XU,Jian ZHAO,Min WEI. Induced Expression of Recombinant β-1, 4-Xylanase and Its Directional Preparation of Xylo-oligosaccharide [J]. Chemistry and Industry of Forest Products, 2020, 40(5): 99-106.
[12]	Yan JIN,Haiyan YANG,Zhengjun SHI,Dawei WANG,Gaofeng XU,Jing YANG. Effect of Alkali-hydroxymethylation Pretreatment on Sugarcane Bagasse Enzymatic Hydrolysis and Fermentation Efficiency [J]. Chemistry and Industry of Forest Products, 2020, 40(4): 86-92.
[13]	Shunli LI,Yu LIU,Fei HUANG,Kai LIU,Honglei CHEN. Synchronous Synthesis of Carbon Nanodots Formed on Pre-hydrolysis of Lignocellulose [J]. Chemistry and Industry of Forest Products, 2020, 40(3): 61-68.
[14]	Xin LI,Qiang YONG. Production of Fumaric Acid from Lignocellulose by Rhizopus oryzae [J]. Chemistry and Industry of Forest Products, 2020, 40(1): 1-7.
[15]	Qiulu CHU,Xueyan CHEN,Kai SONG,Jing WANG,Xiaodong ZHANG,Aiping SHI. Effects of Two-stage Pretreatment on Enzymatic Hydrolysis of Poplar and Adsorption Performance of Lignin [J]. Chemistry and Industry of Forest Products, 2019, 39(6): 68-74.

Analysis of Enzymatic Hydrolysis Process of High-solid Lignocellulose Materials

RichHTML

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

Figures/Tables 5

References 27

Related Articles 15

Recommended Articles

Metrics

Comments